Death in the desert. The sabotage-derailment of "The City of San Francisco"

The completion of the transcontinental railroad in 1869 was a milestone in American history. Seventy years later in August 1939, the westbound, new Southern Pacific Railroad streamlined passenger train "City of San Francisco," was intentionally derailed at high speed in northern Nevada by saboteurs who had moved a rail out of alignment. This resulted in a spectacular wreck, claiming the lives of 24 passengers and crew. Despite the finding of physical and trace evidence; and lengthy, exhaustive investigations by railroad police, the FBI, and forensic scientists, the perpetrators were never brought to justice. Deaths resulting from vandalism and sabotage are among the least common of railroad-related deaths; however, acts of vandalism and violence including train derailment are increasingly frequent in this country. Law enforcement officers and forensic scientists must be able to place these incidents in their proper perspective and to successfully investigate and solve them by employing all means at their disposal.     

Homicidal death following blunt trauma in a vulnerable host, with secondary infections including local tetanus

An uncommon type of homicide resulted from complications of an ordinarily nonfatal injury after a 59-year-old obese, hypertensive, diabetic man was struck in the face with a two-by-four, sustaining a grossly contaminated laceration. It was cleaned and sutured primarily, and a tetanus booster was given. On the fourth hospital day there was evidence of anerobic wound cellulitis, including Clostridium tetani. The wound was surgically debrided, but 2 days later the patient developed local tetanus. Only then was it discovered that he had never been immunized against tetanus. He did not develop systemic tetanus, but 2 days later he died with bronchopneumonia and sepsis. The assailant was indicted for involuntary manslaughter, but after a contentious trial he pleaded "no contest" to a reduced charge. The decedent was a vulnerable host, his contaminated facial laceration initiating an unbroken course of events that led to his death.     

DNA Analysis of Natural Fiber Rope*

Abstract: When rope is found at a crime scene, the type of fiber is currently identified through its microscopic characteristics. However, these characteristics may not always unambiguously distinguish some types of rope from others. If rope samples contain cells from the plants of origin, then DNA analysis may prove to be a better way to identify the type of rope obtained from a crime scene. The objective of this project was to develop techniques of DNA analysis that can be used to differentiate between ropes made from Cannabis sativa L. (hemp), Agave sisalana Perrine (sisal), Musa textilis Née (abaca, “Manila hemp”), Linum usitatissimum L. (flax), and Corchorus olitorus L. (jute). The procedures included extracting the DNA from the rope, performing polymerase chain reaction (PCR) using the extracted DNA as a template, and analyzing the DNA products. A primer pair for PCR, chosen from within a chloroplast gene for the large subunit of ribulose bisphosphate carboxylase/oxygenase, was designed to be specific for plant DNA and complementary to the genes from all five plants. The resulting PCR fragments were approximately 771 base pairs long. The PCR fragments, distinguished through base sequence analysis or restriction enzyme analysis, could be used to identify the five different rope types. The procedure provides a useful addition to visual methods of comparing rope samples.