Earmarking as a Strategy Against Budgetary Constraints: San Francisco's Children's Amendment

The passage and implementation of the San Francisco Children's Amendment, or Proposition J, in 1991 represents an important expansion of the increasingly common strategy of budgetary earmarking by human service organizations. The core of the initiative was a city charter amendment that earmarked a portion of existing property taxes and established a baseline budget for children's services. Of these provisions, the baseline budget has proven most important in preserving children's services and preventing any substitution effects in a time of increasing fiscal stress for the city/ county of San Francisco. This case also illustrates the role of a strong political appeal in passing technical budgetary initiatives over the objections of elected officials and others. While there was concern about the reduced flexibility created by earmarking a city's budget during a period of retrenchment, this concern was overlooked because of the overwhelming political support for the children's cause. However, conflict between groups was created by the measure as groups fought to be included in the provisions of the amendment and over whether each group had obtained its fair share of funding.     

Population genetic data for 18 STR loci in Costa Rica

Allele frequencies for 18 STR autosomal loci (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, TH01, TPOX, CSF1PO, Penta D, Penta E, D19S433, D2S1338 and SE33) were obtained from a sample of 191-500 unrelated individuals from Costa Rica, Central America.     

Male amelogenin dropouts: phylogenetic context, origins and implications

Several commercial PCR multiplex kits incorporate the amelogenin locus for the purpose of human gender identification. Consequently, erroneous results in the electropherogram profile of this locus can carry important forensic implications. In this study, dropout of the amelogenin Y allele was detected in 5 out of 77 phenotypically normal Kathmandu males using the AmpFlSTR Identifiler kit. A battery of male-specific markers including SNPs, STRs, STSs, and a minisatellite were amplified for the five amelogenin null samples in order to delineate the breakpoints of the deletions as well as assess the overall integrity of the Y-chromosome. This study represents the first to examine the haplogroup affiliation of the AMGY deletions. The analyses performed suggest a single origin for the five deletions as indicated by their allocation to a specific Y-haplogroup (J2b2-M241), related Y-STR haplotypes and identical regional localization of breakpoints. The age estimated from the microsatellite variation for the amelogenin deletions (if they are associated by descent) is approximately 6.5+/-3.3 ky, younger than the previously reported related age of the M241 haplogroup representatives (13-14 ky). Our data in combination with previous publications suggest a concentration of afflicted individuals in the Indian subcontinent, possibly as a result of common ancestry. The elevated incidence of the amelogenin dropout in these populations accentuates the need to utilize other loci for gender determination in order to obtain an accurate set of inclusion criteria in forensic casework.