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This report describes the validation of a two phase cell recovery technique for the elution of two common cell types, epithelia and spermatozoa, from frequently examined items submitted as part of sexual assault casework. Furthermore, separation of cell types prior to microscopic examination of cell pellets improves the scientist's confidence in observing and scoring spermatozoa that may be present. During the validation, Orchid Cellmark's Sperm Elution© method consistently recovered a greater number of spermatozoa from simulated sexual assault items and swabs taken following consensual sexual intercourse compared to a water extraction technique. On average the Sperm Elution method recovered over twice the number of spermatozoa compared to the water method. The ability to separate the cell types present allows a rapid microscope slide search for spermatozoa and faster DNA extraction protocol in comparison to Cellmark's previous preferential method. 相似文献
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Difficulties can arise when screening dark casework items for blood, a poor contrast between blood and the background can mean stains are not always evident. Typical indirect searching methods can be time consuming and may result in potentially important bloodstains being missed. Luminol, fluorescein, hydrogen peroxide, ultraviolet light and infrared photography were tested in an effort to find a rapid and efficient blood search tool for direct application to dark surfaces. Methods were compared in their sensitivity, specificity, ability to work on various surface types and their effect on DNA extraction and typing. Along with experimental results, the ease of use, costs and the health and safety considerations were also compared. Hydrogen peroxide was determined to be the most effective method. However, where blood was likely to be dilute, luminol was proposed due its greater sensitivity. 相似文献
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Jamie S. Davidson 《The Pacific Review》2013,26(1):75-99
Abstract This article explores to what extent to local pro-reform actors matter in Indonesia through the prism of anti-corruption campaigns in the country's regions. I argue that the rash of anti-corruption campaigns and related trials involving legislative members, especially from mid-2004 onward, can be attributed neither to the resources lavished on anti-corruption organizations based in Jakarta, nor to the popularity of President Yudhoyono's anti-corruption rhetoric. Instead, it can be traced to a particular anti-corruption campaign that began in earnest in 2002 in Padang, West Sumatra. Using a multi-dimensional approach, a small group of activists relentlessly pursued their newly elected provincial legislators to be accountable to their democratic mandates and as important, to respect the rule of law pursuant to new national anti-corruption legislation. The guilty verdicts of May 2004 galvanized similar groups across the country to investigate their respective legislative bodies. This exemplary case of societal accountability also demonstrated the leverage activists can gain over local politicians when they forge coalitions with other elite actors, especially those in Jakarta. I further explore two anti-corruption cases in the province of West Kalimantan to place post-Padang developments in their proper perspective. If hopes were raised that regional anti-corruption movements–based on the Padang model–might accomplish more than sensational trials but help consolidate democracy at the regional level by holding elected officials accountable, these two examples show how fleeting these expectations might be. The trials that took place but which produced no convictions resulted from the fallout of local political tussles, and not from local civil society organizations galvanized by the ideals of transparency and good governance. 相似文献
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Nicholas Charron 《发展研究杂志》2013,49(9):1472-1493
The analyses in this study demonstrate a more nuanced understanding of a previously understood phenomenon – that openness has a negative relationship with corruption. It is argued that this relationship is substantially influenced by the domestic context, a relationship that has been underdeveloped by previous empirical studies. Focusing on social and political integration, I find that the effect of openness on corruption is conditioned by domestic institutions. The empirical evidence suggests that while political and social openness have a significant impact in combating corruption given a free press, the impact of such international forces are negligible in cases where press freedoms are low. 相似文献
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Nicholas Farrelly 《当代亚洲杂志》2013,43(3):520-522
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Nicholas Ross Smith 《European Security》2013,22(4):525-540
This paper evaluates the competitiveness of the European Union (EU) and Russia's regime preferences in their foreign policies towards Ukraine in the scope of the on-going Ukraine crisis. It is argued that the underpinning geopolitical environment Ukraine currently resides in, wedged between two much larger powers (the EU and Russia), renders it a vulnerable target state for regime promotion from both sides. Indeed, since the 2004 Orange revolution in Ukraine, both the EU and Russia have had discernible regime promotion strategies in their foreign policies. The EU's regime promotion has focussed on facilitating democracy in Ukraine, along with more material interests (trade and strategic aims) while Russia has reacted with increasingly zero-sum policies which pursue its preference for having a loyal and Russian-facing regime in Ukraine. Ultimately, the increasing competitiveness of the EU and Russia has been a key factor in the onset of the Ukraine crisis, which offers important insight into the relationship between large powers and the smaller third states which lie in their overlapping spheres of influence. 相似文献
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