Validation of Half‐Reaction Amplification Using Promega PowerPlex® 16*

Abstract: DNA amplification is a fundamental yet costly process used in DNA analysis. This study evaluated half‐reaction amplification (12.5, 12, and 13 uL) using the Promega Powerplex^® 16 Kit with the hope of reducing sample analysis costs by half. A sensitivity study was completed, along with the testing of various blood stain samples including those with low (<0.40 ng) and high DNA concentrations (>3.0 ng), peak height imbalances, and allelic drop‐out. Also, 467 samples submitted to the MUFSC laboratory for testing were analyzed. Results indicate that half‐reaction amplification produced higher quality profiles than full‐reactions. Average peak heights increased by 85%, peak height imbalances improved, and drop‐out was eliminated in 75.8% of samples. Only eight of 467 case samples required re‐amplification, a success rate of 94% was observed, and the repeat rate decreased significantly. Finally, a DNA input of 0.25–1.0 ng is ideal for half‐reaction amplification.     

Anticipated shaming and criminal offending

Criminological research suggests that informal sanctions like shaming may have a stronger influence on crime than do formal sanctions, but research has yet to examine whether anticipated shaming may mediate the relationship between crime and variables derived from dominant micro-level theories. The present paper argues that variables derived from learning, control, strain, and deterrence theories influence criminal offending via their effect on anticipated shaming. Using data collected from a sample of young adults, results from both tobit and path analyses suggest that the prospect of shaming among friends and family bears a stronger direct relation to criminal intent than do more commonly examined variables and that the effect of such variables on criminal intent is largely indirect, mediated by anticipated shaming. We therefore suggest that crime control efforts might benefit from incorporating a greater role for Braithwaite's conception of reintegrative shaming.     

Power Relations in Employment Disputes

This article reconceptualizes the operation of power relations in employment disputes. We draw on Foucault's theory of neo‐liberal governance to inform our analysis of empirical data exploring how low‐income workers make decisions about whether to engage with the Employment Tribunal system. Particular focus is placed on the ways the state governs employment disputes to achieve ideologically driven objectives. We conclude: first, that power relations in employment disputes operate across a range of institutions and individuals, and that the state's role is powerful and ongoing; secondly, that power relations operate to shape not just the objective context that workers find themselves in when experiencing an employment dispute but also workers' subjective moral codes about appropriate courses of action to take; and thirdly, that despite the powerful influence of the state, workers continue to hold non‐economic values that guide their perception of the appropriate basis for relations between employers and workers.     

15 years later: Post 9/11 support for increased security and criminalizing muslims

Islamophobia appears to be growing, becoming a mainstream ideology in the United States. Despite the current spike in anti-Islamic sentiment and fear of the Islamic “other,” these ideas are not new and can be traced back to the September 11, 2001, terrorist attacks. Using survey data collected only months after the attacks (N = 422), this study uses an integrated threat framework to examine predictors of public support for increased security in the United States. The results show that perceived personal threat and support for policies that criminalize Muslims predict support for security policies that compromise civil liberties. This is especially true among older individuals, conservatives, individuals less inclined towards individual freedom, and those living in the Northeast or in the South.     

Fatalities Due to Failure of Continuous Subcutaneous Insulin Infusion Devices: A Report of Six Cases

Diabetes mellitus type 1 and type 2 are diseases characterized by impaired regulation of blood glucose due to decreased insulin production and insulin resistance, respectively. Management of diabetes mellitus often requires injection of exogenous insulin. Continuous subcutaneous insulin infusion (CSII or insulin pump) is a diabetes treatment modality utilizing a device to aid in regulation of glycemic control. Malfunctions in device components can have rare fatal consequences. Described in this report are six fatalities due to one such malfunction, the failure of plastic cannulas of CSII devices to penetrate the skin and deliver insulin, resulting in fatal diabetic ketoacidosis (DKA). The cases derive from four different death investigation systems. For each case, scene and autopsy findings are presented, as well as selected toxicology and histology findings. These cases illustrate the importance of careful examination of CSII devices in death investigations and introduce a discussion on discrepant manner of death classifications.     

Forensic Inspection of Sensitive User Data and Artifacts from Smartwatch Wearable Devices

Wearable devices allow users the ability to leave mobile phones behind while remaining connected to the digital world; however, this creates challenges in the examination, acquisition, identification, and analysis of probative data. This preliminary research aims to provide an enhanced understanding of where sensitive user data and forensic artifacts are stored on smartwatch wearable devices, both through utilization as a connected and standalone device. It also provides a methodology for the forensically sound acquisition of data from a standalone smartwatch wearable device. The results identify significant amounts of data on the Samsung^? Gear S3 Frontier, greater than that stored on the companion mobile phone. An Apple Watch^® Series 3 manual examination method which produces native screenshots was identified; however, the companion mobile phone was found to store the greatest amount of data. As a result of this research, a data extraction tool for the Samsung^? Gear S3 Frontier was created.     

Evangelizing Congress: The Emergence of Evangelical Republicans and Party Polarization in Congress

The realignment of evangelical voters is well‐documented, but religion's impact within Congress is less clear. New data on home churches of members of Congress shows that the realignment of congressional evangelicals, combined with their growth and distinctly conservative legislative behavior, has significantly contributed to party polarization in Congress. Controlling for other factors, evangelicals are significantly more conservative than members of other religious traditions. This conservatism also has second‐order effects on the polarization of the House, where their more partisan proposals comprise a larger share of the roll‐call agenda when Republicans are in the majority. Moreover, evangelical Republicans in Congress differ significantly from evangelical Democrats in terms their geography, denominations, and experiences prior to Congress.     

Predicting suicidal risk in a cohort of depressed children and adolescents

Background: In children and adolescents with a depressive disorder, predicting who will also go on to exhibit suicide-related behaviors (SRBs), including suicide attempt or self-harm, is a key challenge facing clinicians. Aims: To investigate the relative contributions of depressive disorder severity, hopelessness, family dysfunction, and perceived social support to the risk of suicide-related behaviors. Methods: This was a cross-sectional study of a group of 10-16-year-olds with major depressive disorders and dysthymic disorder. Results: Child-rated depressive disorder symptom severity emerged as the greatest predictor of risk. Hopelessness and family dysfunction were also significant predictors of SRBs. In combination these variables were strong predictors, accounting for 66% of the variance. This is a cross-sectional study design, rather than longitudinal, therefore risk prediction over time was not possible. Conclusions: Understanding the child and adolescents depressive disorder symptom severity from their perspective, their level of hopelessness, as well as their family context is critical in understanding the risk of SRBs. These findings may help to provide direction for targeted interventions to address these clinical risk factors.