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41.
Rasmussen EM Sørensen E Eriksen B Larsen HJ Morling N 《Forensic science international》2002,129(3):209-213
We describe a method to obtain reliable mitochondrial DNA (mtDNA) sequences downstream of the homopolymeric stretches with length heteroplasmy in the sequencing direction. The method is based on the use of junction primers that bind to a part of the homopolymeric stretch and the first 2-4 bases downstream of the homopolymeric region. This junction primer method gave clear and unambiguous results using samples from 21 individuals with length heteroplasmy in the hypervariable regions HV1, HV2 or both. The method is of special value for forensic casework, because sequencing of both strands of an mtDNA region is preferable in order to reduce ambiguities in sequence determination. 相似文献
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43.
Competition policy often asks whether a “fair share” of the benefits from cost savings obtained through mergers or agreements
is passed on to the consumers. We assess the factorsthat determine cost pass-on in some partial-equilibrium oligopoly models,
and show that, although the strength of the pass-on varies from one situation to another, there are some rules of thumb that
give a first approximation of the pass-on rate. We also show that, contrary to common belief and to what is written about
the subject in the European Commission's guidelines on the application of Article 81(3), in most circumstances cost pass-on
does not depend on the price elasticity of demand nor on the market share of the cost saver, and that with competition the
pass-on of firm-specific cost savings is weaker than without.
JEL Classification: C72 (non-cooperative games), D43 (oligopoly), L40 (antitrust policy) 相似文献
44.
Fluorescent SSCP of overlapping fragments (FSSCP-OF): a highly sensitive method for the screening of mitochondrial DNA variation. 总被引:2,自引:0,他引:2
A Salas E M Rasmussen M V Lareu N Morling A Carracedo 《Forensic science international》2001,124(2-3):97-103
The mtDNA analysis (mtDNA) is increasingly being demanded for forensic purposes due to the fact that many times the use of standard nuclear marker fails to analyze degraded samples (such as bones) and specially for the analysis of hair shafts (a common sample in the crime scene). However, analysis of mtDNA sequencing implies a great lab effort when a high number of samples must be analyzed.The present work introduces a novel and reliable method for the screening of mtDNA variation in the first and second hypervariables (HV1 and HV2) regions which we have denominated fluorescent single strand conformation polymorphism (SSCP) of overlapping fragments (FSSCP-OF). FSSCP-OF is based on the basic theory of SSCP analysis and combines two complementary strategies: the use of PCR amplified overlapping fragments and fluorescent detection technology. The overlap region contains a high percentage (50%) of the d-loop mtDNA variation and for this reason, the probability to detect a polymorphic position by SSCP analysis is clearly increased in comparison to conventional SSCP methods due to the fact that the same polymorphic position is usually placed in a different "relative" position in the two overlapped fragments. The use of multicolor fluorescent technology allows also the multiplex amplification of overlapping fragment and its subsequent analysis in an automatic sequencer. We have analyzed 50 samples of unrelated individuals through the FSSCP-OF technique and we have found that using this methodology the probability to distinguish two samples with different sequences is close to 100%. FSSCP-OF has other important advantages with respect to previous screening methods, such as the automation and standardization of the protocols, which is of special interest for the forensic routine. 相似文献
45.
46.
Quantitative Analysis of the Morphological Changes of the Pubic Symphyseal Face and the Auricular Surface and Implications for Age at Death Estimation 下载免费PDF全文
Chiara Villa Ph.D. Jo Buckberry Ph.D. Cristina Cattaneo M.D. Ph.D. Bruno Frohlich Ph.D. Niels Lynnerup M.D. Ph.D. D. M. Sc. 《Journal of forensic sciences》2015,60(3):556-565
Age estimation methods are often based on the age‐related morphological changes of the auricular surface and the pubic bone. In this study, a mathematical approach to quantify these changes has been tested analyzing the curvature variation on 3D models from CT and laser scans. The sample consisted of the 24 Suchey–Brooks (SB) pubic bone casts, 19 auricular surfaces from the Buckberry and Chamberlain (BC) “recording kit” and 98 pelvic bones from the Terry Collection (Smithsonian Institution). Strong and moderate correlations between phases and curvature were found in SB casts (ρ 0.60–0.93) and BC “recording kit” (ρ 0.47–0.75), moderate and weak correlations in the Terry Collection bones (pubic bones: ρ 0.29–0.51, auricular surfaces: ρ 0.33–0.50) but associated with large individual variability and overlap of curvature values between adjacent decades. The new procedure, requiring no expert judgment from the operator, achieved similar correlations that can be found in the classic methods. 相似文献
47.
Ru?ica Tokali? Ivan Buljan Niels Mejlgaard Mar Carrió Alexander Lang Gema Revuelta Ana Maru?i? HEIRRI Consortium 《法庭科学研究(英文)》2021,6(4):320-330
Responsible research and innovation, or RRI, is a concept that aims to bring together society and science for a better future. There are six key elements of RRI: public engagement, gender equality, science education, open access, ethics and governance. Higher Education Institutions and Responsible Research and Innovation (HEIRRI) project aimed to bring the concept of RRI into the educational system. Using state-of-the-art review of good practices, HEIRRI team developed 10 training programmes on RRI for different higher education institution educational levels, including a summer school and a massive open online course (MOOC). We conducted pilot of the trainings and evaluated participants' experiences. Satisfaction with HEIRRI training programmes on responsible research and innovation was high, both for participants and for the trainers, and trainings raised awareness of RRI. Participants' feedback was used to identify areas that need improvement and provided for recommendations for final versions of the HEIRRI training programmes. In order to equip researchers with skills to recognize and apply RRI values, RRI should be included in their education. HEIRRI training is suitable for a range of different disciplines, including forensic science, and is free to use and adjust for specific contexts (available from: https://rri-tools.eu/heirri-training-programmes). 相似文献
48.
Carracedo A Beckmann A Bengs A Brinkmann B Caglia A Capelli C Gill P Gusmão L Hagelberg C Hohoff C Hoste B Kihlgren A Kloosterman A Myhre Dupuy B Morling N O'Donnell G Parson W Phillips C Pouwels M Scheithauer R Schmitter H Schneider PM Schumm J Skitsa I Stradmann-Bellinghausen B Stuart M Syndercombe Court D Vide C 《Forensic science international》2001,119(1):28-41
A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories.Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male).All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data.As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland.Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other. 相似文献
49.
Ever since the successful presidential campaign of Barack Obama in 2008, attention has been drawn to the political impact of social media. However, it remains to be seen whether the successful Obama campaign is the exception or the rule. Our research focuses specifically on the impact of social media on preference voting. First it seeks to establish whether candidates make use of social media during election campaigns and whether voters in turn follow politicians. Afterwards it examines to what extent social media make a difference and yield a preference vote bonus. Theoretically, two types of effects are outlined, namely a direct effect of the number of followers a candidate has and a statistical interaction effect whereby a higher number of followers only yields more votes when the candidate actively uses the social media. To carry out our analysis, we make use of a unique dataset that combines data on social media usage and data on the candidates themselves (such as position on the list, being wellknown, exposure to the old media, gender, ethnicity and incumbency). The dataset includes information on all 493 candidates of the 10 parties that received at least one seat in the Dutch 2010 election. It turns out that candidates are eager to use social media, but that relatively few people follow candidates. There is a significant interaction effect of social media usage and the number of followers, but that effect appears to be relatively small. 相似文献
50.
This article of top civil servants in Danish central and local government sheds new light on politicization. A survival analysis shows that since 1970, the risk that a top civil servant will be replaced has increased. There is no evidence of politicization in central government while city managers' risk of replacement increases both when a new mayor, representing another party and holding an absolute majority, and when the shift of mayor takes place within the same party. We interpret these results as evidence of the adaptability of the merit civil service and political executives' stronger insistence on their authority to make discretionary replacements. 相似文献