The use of the LightCycler for the detection of Y chromosome SNPs

A novel methodology based on PCR monitoring on-line with fluorescent formats using the LightCycler for Y chromosome SNP typing is proposed. The main advantages of the system are the time necessary for the analysis (which is around 20 min), the robustness and the accuracy of the method and especially its sensitivity, which permits the detection of the male component in male-female mixtures up to 1:300 for some of the SNPs.Singleplexes of four different SNPs (M9, sY81, SRY-1532 and SRY-2627) as well as two duplexes (M9 and sY81 on the one hand and SRY-1532 and SRY-2627 on the other) were efficiently implemented. A simultaneous amplification and analysis of the four SNPs is also possible. It seems difficult with the current methodology to implement more than a quadruplex.     

Occupational safety and health in Poland in the 1990s: A regulatory system adapting to societal transformation

The societal transformation underway in Poland createda fundamental challenge to the occupational health and safety system, as the ideological and administrative principles on which it was founded vanished along with the communist-dominated regime. This paper examines the regulatory reform in Poland during the 1990s: its structural elements, implementation record and future prospects. Drawing on five case studies of privatized firms, a mailed questionnaire, and policy and institutional analysis, we find that Poland had considerable success in developing an effective regulatory system for managing occupational health hazards in privatized sector while also achieving considerable socioeconomic progress. The fundamental legitimacy of the regulators and regulatory process, the availability of information about firms and regulatory intents, and the capacity for case-specific decision making, are among the key explanatory factors. The case-specific implementation in Poland is consistent with models advocated by several authors in relation to other industrialized European economies (termed variously as negotiated compliance, tit-for-tat, cooperation-deterrence), despite a uniquely Polish context related to the continuing legacy of the communist era. The study also shows how in Poland a good fit between regulatory institutions and policies on the one hand, and their social context on the other hand, contributes to the effectiveness of the regulatory system.     

Calculation of percent shrinkage in human fetal diaphyseal lengths from fresh bone to carbonized and calcined bone using Petersohn and K?hler's data.

Calculation of age from fetal and newborn remains may be problematic, and when these remains are altered by maceration, decomposition or burning, age may be more difficult to discern. When soft tissue indicators are transformed, then two techniques exist for accurate age determination; dental development, which may prove difficult given the degree of tissue alteration; and appearance, size and fusion of ossification centers, including diaphyseal length, which may yield inaccurate ages if shrinkage is not accounted for. This study is undertaken to facilitate age calculation by systematically re-evaluating diaphyseal shrinkage and determine shrinkage rates from wet to carbonized states and wet to calcined states using Petersohn and K?hler's data, originally published in German and then published in Fazekas and Kósa (1978:362-369). Average shrinkage, standard deviation, minimum and maximum values are calculated for each diaphysis and then for all diaphyses between 4-10 lunar months (LM) and for newborns. Associated values for carbonized diaphyses are: 4 LM--32.50% +/- 12.12%; 5 LM--14.04% +/- 4.44%; 6 LM--6.78% +/- 1.06%; 7 LM--4.18% +/- 0.31%; 8 LM--3.47% +/- 0.42%; 9 LM--3.05% +/- 0.18%; 10 LM--2.46% +/- 0.67%; and in newborns 2.16% +/- 0.29%. Similar values for calcined diaphyses are: 4 LM--40.11% +/- 17.51%; 5 LM--18.29% +/- 4.42%; 6 LM--9.84% +/- 1.27%; 7 LM--9.82% +/- 0.51%; 8 LM--9.42% +/- 0.72%; 9 LM--9.45% +/- 0.33%; 10 LM--8.94% +/- 0.37%; and in newborns 8.96% +/- 0.49%. These findings suggest that percent shrinkage due to carbonization and calcination is greatest in the earliest age groups, decreasing substantially with advancing age. The rates of shrinkage, however, vary by the burning process utilized and age group studied. These general findings are similar to those of Petersohn and K?hler, yet specific values for percent shrinkage vary greatly from values cited in this analysis. These data provide a means to assess the degree of shrinkage that occurs for each diaphysis for each given age group.     

The relationship of compliance with anxiety,self-esteem,paranoid thinking and anger

Abstract

The aim of the study was to assess the relationship of compliance with anxiety, self-esteem, paranoid thinking, and anger. The Gudjonsson Compliance Scale (GCS), the State-Trait Anxiety Inventory (STAI), the Rosenberg Self-Esteem Scale, the Paranoia/Suspiciousness Questionnaire (PSQ), and the Novaco Anger Scale (NAS) were administered to 167 University students Compliance correlated positively with state and trait anxiety, low self-esteem, paranoia and suspiciousness, and negatively with acting out feelings of anger (the Behavioural Domain of the NAS). Multiple regression of the test scores showed that paranoid thinking was the single best predictor of compliance. The implications are discussed. It is important to replicate the study on special populations, such as offenders and psychiatric patients, who have problems with low self-esteem, paranoid thinking, and anger.     

Literatur

Ohne Zusammenfassung     

Differences in Physical Fitness and Body Composition Between Active and Sedentary Adolescents: A Systematic Review and Meta-Analysis

Journal of Youth and Adolescence - Previous research analyzing the differences in physical fitness and body composition between active and sedentary adolescents aged 12–16 has not provided...     

First Generation in Chilean Higher Education: Tension between Access and Inclusion in a Segmented University System

We analysed the Chilean university student selection processes, comparing ‘First Generation’ and ‘Continuist’ students, using the Chilean Higher Education population databases (2000–2015). Findings confirm that 60 percent of participants in the selection process are First Generation students. The data registers an increasing self-exclusion phenomenon. Of the students who did not take the selection test after enrolling, 80 percent are First Generation and 18 are enrolled in a selective university, compared to 44 in the Continuist conglomerate. These differences may be explained by cultural capital in the Bourdieu sense, in conjunction with a diversified, massified and marketalised higher education system.     

Women Sustaining Community: The Politics of Agro-Ecology in Quilombo Tourism in Southern Brazil

This article analyses how a gendered perspective on community-based tourism (CBT) contributes to the realisation of identity and sovereignty of quilombos. It examines the potential sustainability of CBT coupled with agro-ecological practices within the context of gendered identity politics. Drawing from in-depth ethnographic and archival research conducted between February and July 2015 at Campinho da Independência (Rio de Janeiro, Brazil), the article discusses how female leaders integrate sustainable agro-ecological practices and CBT with environmental education in what they call ‘ethno-ecological tourism’. This approach to CBT strengthens a proudly defiant version of Afro-Brazilian ethnic identity vis-à-vis the state.