尸僵再形成的实验性研究

目的观察尸僵再形成中肌节长度的变化。方法应用扫描电镜测定不同条件下取材的40只大鼠股四头肌肌节长度,判断再僵直的强度。结果死后未破坏的尸僵肌节的长度明显小于尸僵再形成时的长度。结论肌节长度与尸僵强度呈负相关,说明肌节长度可确定尸僵强度,从而为推断死亡时间提供依据。     

血管内皮细胞生长因子在法医学中的应用

血管内皮细胞生长因子(VEGF)是损伤愈合过程中启动血管形成的一个特异的有丝分裂原,也是一种有效的血管通透性诱导因子。本文结合有关文献,对当前有关的VEGF分子生物学特性,VEGF与损伤修复的关系及表达规律等方面的研究现状作一简要综述。     

纤维连接蛋白Ⅲ CS片段的剪接、表达以及与创伤修复的关系

纤维连接蛋白在创伤愈合中具有重要的作用 , 其基因在三个区域进行可变剪接 , 分别命名为 EⅢ A,EⅢ B和Ⅲ CS. 它们的功能仍有待于研究 . 本文就纤维连接蛋白的可变剪接片段Ⅲ cs的生物学活性及其在法医学中的应用展望做一粗浅的探讨 .     

脑干损伤后神经元及轴突改变的组织化学观察

采用针刺法造成大鼠脑干损伤,用尼氏体染色、嗜银染色和改良三色法观察脑干神经元及轴突在伤后不同时间的病理改变。结果发现,嗜银染色显示伤后 1～ 3h部分神经纤维不规则增粗、少数断裂, 6h断端膨大呈球形, 15h收缩球较为明显,至 24h收缩球明显且数量增多;改良三色法显示伤后 3～ 6h部分髓鞘与轴突之间的间隙增宽, 15h髓鞘明显弯曲、不完全地附着在轴突表面,甚至剥脱,持续到伤后 24h;尼氏体染色显示神经元核周尼氏体在伤后 24h减少。该结果提示,组织化学染色能观察到脑干损伤后的病理改变,并且有可能用于推断脑干损伤时间。     

混合斑中酸性磷酸酶及精子检出时限的研究

目的 研究探讨性生活后活体阴道内混合斑中酸性磷酸酶（ ACP）及精子的检出时限。方法 采用取材、检验三盲法对 600例阴道拭子进行 ACP及精子的常规检验。结果 ACP最长检出时限 255h,平均检出时限 52h。精子最长检出时限 132h,精子平均检出时限 29h。 ACP检出例中阳性程度在（＋＋）以上占 41.3％,精子检出例中阳性程度为每个视野 6～ 10个占 34.3％。 结论 应用混合斑中酸性磷酸酶及精子的检出时限 ,能为侦审性犯罪案件的法医学鉴定提供客观依据。     

电击伤组织的超微结构观察

目的 观察电流传导路径上器官、组织的超微结构变化。 方法 对 SD大鼠进行实验性低电压电击。结果 触电部位皮肤的上皮细胞胞浆内基质凝固、膜系统断碎;骨骼肌肌小节出现异常收缩带;心肌的变化也较明显,可见局灶性溶解坏死并出现异常收缩带;电流传导路径上外周血管、神经的改变以血管明显,血管内皮细胞胞浆中出现空泡,中膜平滑肌可见溶解坏死,神经纤维仅见部分髓鞘松解。 结论 电击伤组织的超微结构变化可作为诊断电击死组织学变化的补充,为解决无明显皮肤电损伤的电击死案件提供形态学指标。同时也探讨了电击伤超微结构变化的形成机制。     

DNA testing of Klinefelter's syndrome in a criminal case using XY chromosomal STR multiplex-PCR

We report genetic typing of Klinefelter's syndrome applied to casework in forensic DNA testing. In this case, by using extracted DNA from body samples (muscle and bones), we could identify two distinct X alleles in two out of three X-STR loci (HPRTB and ARA), in addition to Y alleles (DYS390, DYS393). The extra X was found to have originated from father, and the victim turned out to have 47XXY Klinefelter's syndrome. The victim was a 30-year-old male, born from relatively elderly parents as a second child. His father was a severe alcoholic and had been malnourished for more than 20 years at the moment of his birth. He exhibited slight mental retardation as a child, and belonged to a criminal group as an adult. The method presented here was useful to accurately diagnose sex chromosomal abnormality instead of conventional chromosomal analysis and Xg blood group typing. A subtype of this syndrome, 48 XXXY or mosaic, for example, could be identified if the intensity of the overlapped X bands were calculated.     

Regional distribution of methamphetamine in autopsied brain of chronic human methamphetamine users

We measured levels of methamphetamine and those of its metabolite amphetamine in 15 autopsied brain regions of 14 human methamphetamine users. Only slight regional differences were observed in drug concentrations among the brain areas. Although, some redistribution of the drugs probably occurred postmortem, these data suggest that methamphetamine might not be preferentially retained in dopamine-rich brain areas but is heterogenously distributed in brain of chronic human users of the drug. The possible pharmacological actions of methamphetamine in both dopamine-rich and poor brain areas of chronic drug users need to be considered.     

Alpha-phenylethylamine in illegally produced amphetamine.

The possibility of simultaneous synthesis of alpha-phenylethylamine and amphetamine from mixture of acetophenone and benzylmethylketone was studied. The structures of specific impurities were predicted and these compounds were synthesized and finally found in reaction mixtures, as well as in the final product. The data collected by gas chromatography, proton and carbon magnetic resonance, Fourier transform infrared spectrometry and mass spectrometry are presented.