Optimization of the separation of organic explosives by capillary electrophoresis with artificial neural networks

The separation of 12 explosives by capillary electrophoresis was optimized with the aid of artificial neural networks (ANNs). The selectivity of the separation was manipulated by varying the concentration of sodium dodecyl sulfate (SDS) and the pH of the electrolyte, while maintaining the buffer concentration at 10 mM borate. The concentration of SDS and the electrolyte pH were used as input variables and the mobility of the explosives were used as output variables for the ANN. In total, eight experiments were performed based on a factorial design to train a variety of artificial neural network architectures. A further three experiments were required to train ANN architectures to adequately model the experimental space. A product resolution response surface was constructed based on the predicted mobilities of the best performing ANN. This response surface pointed to two optima; pH 9.0-9.1 and 60-65 mM SDS, and pH 8.4-8.6 and 50-60 mM SDS. Separation of all 12 explosives was achieved at the second optimum. The separation was further improved by changing the capillary to an extended cell detection window and reducing the diameter of the capillary from 75 microm to 50 microm. This provided a more efficient separation without compromising detection sensitivity.     

Protesting a perceived injustice: Sex discrimination and the restoration of equity

In this paper we propose a theoretical model that examines the relationship between resources and significant others in people's attempts to restore equity. Viewing the restoration of equity as a complex process, we integrate resource mobilization and equity arguments in order to better understand how people come to dispute and settle fair terms of exchange in allocative relationships. With individual-level data, we examine sex discrimination complaints and show that protest reactions against a perceived injustice are influenced by the level of resources a person controls. We find that the more resources a person controls, the better able she or he is in managing the protest and negotiation for equity. Further, resources also protect complainants from reprisals by the source of the inequity. These findings provide insight into the process by which people attempt to restore equity, and they suggest that the examination of real-life inequitable relations enhances our understanding of reactions to inequity.     

Behavioral and Verbal Responses of Unabused Externalizing Children to Anatomically Detailed Doll Interviews

To see if unabused children with externalizing behavior disorders display more sexual behaviors and verbalizations than other children in their interactions with anatomically detailed dolls, sixteen 3- to 6-year-olds were compared with 44 nonexternalizing controls. A five-phase structured, detailed interview, and comprehensive coding of videotapes by external raters, was completed on 17 behaviors and verbalizations. As would be expected, externalizing children exhibited more overall activity than non-externalizing children. Also, externalizing children exhibited more behavioral sexual aggression during the body inventory phase, but no other differences in sexual behaviors or verbalizations occurred. Results suggest that most normative data on AD dolls generalize to externalizing children.     

Alternative direct-to-amplification cell lysis techniques for forensically relevant non-sperm cells

While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Recently, an alternative, direct-to-amplification sperm lysis method (using 1 M NaOH) was identified. However, a direct cell lysis method for non-sperm cells has not been identified yet. Thus, the primary objective of this work was to find an alternative method that is quick, inexpensive, and does not require multiple purification steps for the lysis of non-sperm cells in sexual assault samples. In this study, vaginal swab samples were lysed with the control method, prepGEM™, as well as six alternative reagents: alkaline buffer with 25–200 mM NaOH, high-salt stain extraction buffer, modified radioimmunoprecipitation assay (RIPA) buffer, mammalian protein extraction reagent (M-PER™), digitonin buffer, and urea/thiourea buffer. Quantification using Quantifiler® Trio of vaginal and semen lysates revealed that the alkaline (25 mM NaOH) and M-PER™ methods were efficient for the lysis of vaginal epithelial cells without substantial sperm cell lysis. Following quantification, analysis of STR profiles from vaginal lysates revealed that the M-PER™ method showed promising results across all metrics examined, including the percentage of detected STR alleles, mean peak heights, peak height ratio, and interlocus balance. Thus, this method was recommended as an alternative to the traditional differential lysis method for non-sperm cells given its ability to produce amplification-ready lysates without any DNA purification step.     

Can agricultural intensification help attain Sustainable Development Goals? Evidence from Africa and Asia

Market-oriented agricultural intensification is a major development strategy, yet its alignment with sustainable development goals (SDGs) is unclear. We apply indicators for SDG 2 (eradicate hunger) regarding income, food production, food security and land tenure to recent intensifications in Rwanda and Laos to reveal their disaggregated impacts. We find while market-oriented intensification may generate poverty reduction, it also exacerbated marginalisation and poverty through various forms of land tenure insecurity. Ethnicity and gender were influential factors in Rwanda, and post-conflict resettlement policies in Laos. We discuss implications for development practice and selection of suitable indicators to reflect the ambition of the SDGs.     

Representative Bureaucracy: A Lever to Enhance Social Equity,Coproduction, and Democracy

Scholars and practitioners have long questioned whether the race, ethnicity, and gender of public bureaucrats matter to the efficacy and legitimacy of public services. Representative bureaucracy theory and research provide a growing body of empirical evidence that it does. This article examines some of the rich scholarly work that has been generated on representative bureaucracy and its implications for practice. A significant aspect of recent research focuses on the notion of symbolic representation, whereby the mere existence of a passively represented bureaucracy can itself improve outcomes by influencing the attitudes and behaviors of clients, regardless of bureaucratic actions or results. This article is intended to help both students and public managers understand the importance of representativeness in public organizations for a broad spectrum of practices and goals, from the coproduction of services to democratic rule.     

Past and Present: From Misunderstanding Sexuality to Misunderstanding Gender Identity in Australian Refugee Claims

This paper analyses the past and present treatment of people applying for asylum on the grounds of their sexual orientation in Australia. Despite having relatively progressive legislative measures for lesbian, gay, bisexual, transgender, and intersex (LGBTI) citizens, the treatment of refugees applying for refugee status based on their sexual orientation has historically been dogged by questionable and biased decision‐making. It is argued that early claims were initially plagued by a number of issues, notably the employment of discretion reasoning and errors in credibility assessment. The second section of this paper takes a deeper look at claims made in Australia between 2016 and mid‐2018 to assess whether there has been any improvement in decision‐making. It is argued that Australian decision‐making for sexual orientation‐based claims has demonstrably improved, encouraged by the introduction of new departmental guidelines for assessing such claims in 2016. However, it emerged in later claims that some decision‐makers continue to confuse sexuality and gender identity, demonstrating that issues remain for applicants in Australia. Ultimately, it is argued that in order to continue to improve decision‐making, the Australian Immigration Department and tribunals need to be more transparent about the development and implementation of decision‐making guidelines.     

How does diversity affect public organizational performance? A meta-analysis

Research in public administration examining the effects of diversity on public organizational performance has produced mixed results. However, the lack of a comprehensive theoretical framework has failed to provide an explanation for the mixed diversity effects. This study introduces a systematic analytical model, Categorization-Elaboration Model (CEM), to help identify the contextual constructs which can promote the positive effects of diversity on public organizational performance. A meta-analysis is conducted on 37 quantitative studies to test the empirical validity of a CEM constructed theoretical model with 253 effect sizes which will promote a better understanding of the circumstances or contexts that lead to the benefits of diversity within public sector organizations. The empirical results of meta-regression point to the appropriate range of contextual factors which can alleviate the potential negative effects of diversity and promote its overall positive effects.     

How Russia's Internet Research Agency Built its Disinformation Campaign

In this article we analyse features of the information influence operations run by the St. Petersburg based Internet Research Agency, targeted at Europe. Informed by publicly available ‘open source’ data, the analysis delineates three key tactics that underpinned their disinformation campaign: account buying; ‘follower fishing’; and narrative switching. Both individually and collectively these were designed to build the reach, impact and influence of the ideologically loaded messages that social media account operators authored and amplified. The particular value of the analysis is that whilst a lot of recent public and political attention has focussed upon Kremlin backed disinformation in respect of the 2016 United States presidential election, far less work has addressed their European activities.