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201.
This article focuses on the interaction between government policy and public attitudes in order to explain why the Howard government was reasonably pragmatic given that it maintained enough popular support to win four successive federal elections from 1996, and why such a relationship is crucial to evaluating policy trends in Australia's liberal democracy in terms of understanding both policy possibilities and limitations no matter how flawed or divisive certain policies may appear to be.  相似文献   
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Network governance can enhance democratic practice by furnishing new routes for actors to deliberate, make, and execute public policy. But it is hindered by a lack of political oversight, limited democratic competence of new organizational forms, and informality of operation. Little research has been conducted on the democratic performance of governance networks, and the methodology is poorly developed. Quality-of-democracy studies of national governmental and political systems offer a starting point. Their criteria-based method is useful in accessing the democratic "hardware" of formal entities, such as partnerships and hybrids, but it does not enable data to be gathered on democratic "software"—the informal day-to-day practices of actors in networks. Interpretive approaches offer a way forward. Narrative analysis, qualitative interviews using a criteria-based instrument, and Q-methodology provide routes into democratic software. They enable the researcher to move beyond the analysis of institutional nodes and to understand the democratic performance of the wider governance network.  相似文献   
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Abstract: The mooting late in 1976 of changes in the powers of the Commonwealth to retire its employees on grounds of redundancy sharpened interest in the legal dimension of security in public employment in Australia. If it were to become law, the Commonwealth Employees (Redeployment and Retirement) Bill 1976 would modestly consolidate the legislative powers of the Commonwealth to rationalize and retrench employees in line with changes in its operational requirements, but it would by no means resolve all the problems of legal uncertainty and insecurity that the present position allows.  相似文献   
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This paper examines the potential of infrared chemical (hyperspectral) imaging as a technique for the forensic analysis of automotive paint chips in particular, and multicomponent (e.g., layered) samples in general. Improved sample preparation procedures for the infrared analysis of paint chips are detailed, with the recommendation that where mounting resins are chemically incompatible with the sample, it is better to mount and section the sample in a soft wax from which the sections can be removed and pressed into a KBr disk for transmission analysis. Infrared chemical images of multilayered paint chips have been successfully obtained, with the chief advantage over conventional infrared analysis being that thousands of infrared spectra are collected in a few minutes across the whole sample, at a spatial resolution of around 5 microm. As with conventional infrared spectroscopy, chemical species can be identified from their spectra, but the wealth of information available can be also extracted in a number of different ways that make multicomponent spectral (and hence chemical) comparisons between two samples easy to visualize and understand. In one approach, the infrared chemical images of two paint chips being compared side-by-side can be viewed as a "movie," in which each frame is an intensity map of the two samples at a given wavenumber (frequency) value. In another approach, the spectra (pixels) in the image files are classified into chemically similar groups, resulting in a "cluster" image that makes it possible to simultaneously compare all of the layers in two paint chips. These methods are applicable to other multicomponent samples, and also to other chemical imaging techniques.  相似文献   
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The purpose of these studies was to evaluate the performance characteristics of the Cozart Microplate Enzyme Immunoassay (EIA) for the determination of methadone in oral fluid from patients in a drug misuse treatment program. Oral fluid specimens were collected using the Cozart RapiScan Collection system from 198 donors who were receiving treatment for their addiction and were monitored for drug misuse. Oral fluid specimens were also collected from forty volunteer donors who were not drug users. The specimens were analyzed in the laboratory by EIA and then analysed for methadone and its main metabolite EDDP by gas chromatography-mass spectrometry (GC-MS). A total of 103 samples were confirmed positive for methadone. The Cozart Microplate EIA for d-Methadone in oral fluid using a cutoff of 30 ng/mL in diluted oral fluid had a sensitivity of 91.3% +/- 2.8% and a specificity of 100% +/- 1.0% vs. GC-MS.  相似文献   
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A feasibility study of short tandem repeat (STR) genotyping of telogen phase hairs in particular, and hair shaft in general, is presented. A number of extraction procedures in common use were investigated and the quantities of nuclear DNA (nuDNA) delivered were quantified via a real-time PCR assay. The extracts were subjected to two variations on AmpFlSTR Profiler Plus PCR amplification strategies (extended cycles, two rounds of PCR) and the genotypes compared. Nuclear DNA was found to persist in human hair shafts, albeit at very low levels. Full Profiler Plus profiles consistent with the hair donor were obtained from 100 mg hair shaft samples (bleached and unbleached). These were, however, mixed profiles, indicating low copy number (LCN) contamination in the extracts. Single telogen hair clubs and single hair shafts delivered partial profiles with usually only one allele of heterozygous loci. Telogen phase hairs yielded the same amount of nuDNA (and no more) as hair shafts (either anagen or telogen). Whether hair shafts dissolved or not in lysis buffer had no effect on either the quantitated yield of DNA or on the chance of obtaining a correct genotype. These results provide evidence that genomic DNA resides on the exterior of the hair shaft and we use this information to suggest an optimal procedure for nuDNA extraction from keratinised hair samples: soaking hairs in simple digestion buffers containing Tris-HCl, a salt and a chelating agent without prior cleaning of the hair shafts. It is proposed that cleaning removes most of the recoverable DNA. This procedure was applied to obtain genotypes from 3 cm hair shafts which matched reference profiles from the donors at up to 9 out of 10 AmpFlSTR Profiler Plus STR loci. When the genotyping success was measured by counting the number of matches between the two dominant alleles at each locus for each extract with the reference DNA profile of the hair donor, the success was found to be highly dependent on the donor. The number of matching alleles varied between not less than 10 for one donor to no more than two for another donor. These results may well be linked to the environmental experience of the hairs from each donor prior to removal.  相似文献   
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