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811.
812.
P.M. Vallone M.C. Kline D.L. Duewer A.E. Decker J.W. Redman J.C. Travis M.V. Smith J.M. Butler 《Forensic Science International: Genetics Supplement Series》2008,1(1):80-82
National Institute of Standards and Technology SRM 2372 human DNA quantitation standard has been produced to support the need for a human-specific DNA quantitation standard in forensic casework and calibration of new quantitative polymerase chain reaction (qPCR) assays. The conventional DNA concentration has been assigned with one of the U.S. National Reference UV/Visible Spectrophotometers, assuming an absorbance of 1.0 at 260 nm equals 50 ng/μL of double stranded DNA. In addition, an interlaboratory study has been conducted, to verify that the SRM 2372 materials perform well in currently used DNA quantitation assays by the forensic DNA community. Each unit of SRM 2372 consists of three well-characterized DNA extracts. Component A is a single-source human male material derived from blood. Component B is a multiple-source human female material derived from blood. Component C was purchased as a purified unsheared genomic human DNA (Sigma-Aldrich Co., St. Louis, MO) obtained as a lyophilized human genomic extract and has both male and female donors. SRM 2372 is intended to enable the comparison of DNA concentration measurements across time and place. Manufacturers can use SRM 2372 to validate the values assigned to their own reference materials. Individual forensic laboratories can use SRM 2372 to validate DNA quantitation methods and to verify the assigned concentration of in-house or commercial DNA calibration standards. 相似文献
813.
814.
Indian Peafowl (Pavo cristatus) tail covert feathers were studied to investigate the difference between shed and plucked feathers in the context of wildlife offence cases involving the killing of the Indian national bird for the purpose of plucking feathers. Plucked feathers were distinguished from shed feathers by examining their roots under low magnification of a stereoscopic microscope. A chemical test to show the presence of blood on the roots of plucked feathers was used to corroborate the plucked origin of feathers. 相似文献
815.
Book Reviews in This Article:
America's Health Care Revolution. Who Lives? Who Dies? Who Pays? By Joseph A. Califano, Jr.
American Medicine: The Power Shift . By Eli Ginzberg
Health Services in the United States, 2d ed. By Florence A. Wilson and Duncan Neuhauser
The U.S. Health Care System. A Look to the 19905 . Edited by Eli Ginzberg.
Handbook of Health Care Risk Management . By Glenn T. Troyer and Steven L. Salman
Health Care Risk Management: Organization and Claims Administration . By Gary P. Kraus
Medicolegal Aspects of Critical Care . Edited by Katherine Benesch, Norman S. Abramson, Ake Grenvik, and Alan Meisel
Ethics and Critical Care Medicine . Edited by John C. MosLop and Loretta Kopelnian
The mentally disabled and the Law , 3d ed. By Samuel Jan Brakel, John Parry, and Barbaa A. 相似文献
America's Health Care Revolution. Who Lives? Who Dies? Who Pays? By Joseph A. Califano, Jr.
American Medicine: The Power Shift . By Eli Ginzberg
Health Services in the United States, 2d ed. By Florence A. Wilson and Duncan Neuhauser
The U.S. Health Care System. A Look to the 19905 . Edited by Eli Ginzberg.
Handbook of Health Care Risk Management . By Glenn T. Troyer and Steven L. Salman
Health Care Risk Management: Organization and Claims Administration . By Gary P. Kraus
Medicolegal Aspects of Critical Care . Edited by Katherine Benesch, Norman S. Abramson, Ake Grenvik, and Alan Meisel
Ethics and Critical Care Medicine . Edited by John C. MosLop and Loretta Kopelnian
The mentally disabled and the Law , 3d ed. By Samuel Jan Brakel, John Parry, and Barbaa A. 相似文献
816.
817.
A. Barbaro P. Cormaci A. Barbaro 《Forensic Science International: Genetics Supplement Series》2008,1(1):135-139
X-STRs have been proven to be useful in case of deficiency paternity testing and in effective mother-son kinship and father-daughter testing.In the present study, we investigated the distribution of 8 X-STRs loci DXS8378, HPRTB, DXS7423, DXS7132, DXS10134, DXS10074, DXS10101, DXS10135 in an Italian population sample, using the Mentype® Argus X-8 PCR Amplification Kit (Biotype).Samples for the study were obtained form 200 unrelated healthy individuals belonging to Calabria (South Italy) population since at least 3 generations. 相似文献
818.
819.
Maura Barbisin Ph.D. Rixun Fang Ph.D. Cristin E. O’Shea B.S. Lisa M. Calandro M.P.H. Manohar R. Furtado Ph.D. Jaiprakash G. Shewale Ph.D. 《Journal of forensic sciences》2009,54(2):305-319
Abstract: The Quantifiler® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (CT) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR® Amplification Kit to obtain interpretable short tandem repeat profiles. 相似文献
820.
A range of fibre samples was measured using J&M MSP400 and J&M MSP800 microspectrophotometers across the visible and UV/visible wavelength ranges respectively. The first derivative of the absorbance spectra was then calculated and studied. When the absorbance spectra produced for some samples were broad and featureless, the first derivative spectra provided more points of comparison that facilitated discrimination. For many of the samples, calculating the first derivative did not result in any additional discrimination due to the high number of points of comparison present in the absorbance spectra. However, for the samples that exhibited a high level of intra-sample colour variation (e.g. through uneven dye uptake common in cotton and wool, etc.), which was evident in the absorbance spectra, the associated first derivative spectra highlighted this variation between the fibres and could potentially have resulted in false exclusions. The results show that whilst calculating first derivative can be a useful aid in the comparison of spectra, a high degree of caution is required when applying this method to fibres which exhibit a large intra-sample variation in colour. 相似文献