Application of solid-phase microextraction to the profiling of an illicit drug: manufacturing impurities in illicit 4-methoxyamphetamine

This article describes the application of solid-phase microextraction (SPME) to the recovery of manufacturing by-products and impurities from an illicit drug seizure. The preparation chosen for examination using this technique contained 4-methoxyamphetamine, an hallucinogenic amphetamine that has been encountered frequently in South Australia. Compounds found in the PMA preparation included 4-methoxyphenol, 4-methoxybenzaldehyde, 4-methoxyphenyl-2-propanone, 4-methoxyphenyl-2-propanol, 4-methoxyphenyl-propene, and (tentatively) 4-methyl-5-(4'-methoxyphenyl) pyrimidine. The presence of these compounds suggests that the active drug was prepared from 4-methoxybenzaldehyde via 4-methoxyphenyl-2-propanone using a Leuckardt reductive amination. In this instance, SPME was found to be a simple, rapid, and non-destructive recovery technique that gave results complementary to those provided by conventional liquid-liquid extraction. There is an indication that SPME might find application in profiling of illicit drugs.     

A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework.

A validation study was conducted to demonstrate that deoxyribonucleic acid (DNA) could be successfully extracted from human nail material and analyzed using short tandem repeat (STR) profiling and/or mitochondrial DNA (mtDNA) sequencing. This study involved the development of a DNA extraction protocol that includes a cleaning procedure designed to remove external contaminants (e.g., biological, chemical). This protocol was used to test human nail material that had been soaked in whole blood from a second donor and coated with gold-palladium to simulate scanning electron microscopic analysis. The results showed no indication of a mixture and were consistent with that of the nail donor. Fresh human nail material usually yielded both STR profiles and mtDNA sequence information; however, aged human nail material (approximately eight years old) yielded only mtDNA sequence information. Upon completion of the validation study, the extraction protocol was used for the analysis of a torn fingernail fragment recovered from the scene of a violent homicide in 1983. A partial STR profile and mtDNA sequence information indicated that the fingernail fragment was excluded as originating from the suspect and was, in fact, consistent with originating from one of the victims.     

Chorionic villus sampling prior to pregnancy termination, a tool for forensic paternity testing.

Chorionic villus sampling (CVS), prior to pregnancy termination (pre-termination CVS), is suggested as a tool for forensic paternity testing. Unlike the abortion material, which consists of ruptured tissues of fetal and maternal origin, extra-embryonic membranes obtained through CVS can provide an uncontaminated source of fetal tissue for genotyping. We discuss the possibility of confined placental mosaicism (CPM) and its implications on the polymerase chain reaction (PCR) based analyses of short tandem repeats (STRs) and the D1S80 loci.     

Wischnewski ulcers and acute pancreatitis in two hospitalized patients with cirrhosis, portal vein thrombosis, and hypothermia.

Accidental hypothermia has been described in the forensic literature but reports of occurrence in hospitalized patients are rare. Associated anatomic lesions include acute hemorrhagic pancreatitis and characteristic acute gastric ulcers termed Wischnewski ulcers. We report here two patients with cirrhosis and ascites; one also had hepatocellular carcinoma. Portal vein thrombosis, acute hemorrhagic pancreatitis and Wischnewski ulcers were present in both. The clinical records documented hypothermia that progressed over several days. Temperature nadirs of 31.0 degrees C (87.8 degrees F) and 32.2 degrees C (90.0 degrees F) were recorded in each patient, respectively, one day before death, although each transiently reached temperatures that did not register on standard monitoring devices. This is the first report that chronicles antemortem body temperatures in hypothermic patients with Wischnewski ulcers and pancreatitis at autopsy. Also, the association of these findings with portal vein thrombosis and cirrhosis has not been previously described. We discuss this constellation of findings with regard to possible mechanistic interrelations.     

Death fantasies of father-absent and father-present late adolescents

Parental perceptions of father-absent and father-present late adolescents were studied by having them create stories to selected pictures from the Thematic Apperception Test and the Michigan Pictures Test. Ratings on the manifest thematic content of the stories revealed that father-present females, but not males, introduced themes of death and loss involving the parents with significantly greater frequency than did late adolescents who had actually lost their fathers. One of the tasks of adolescence, as described in psychoanalytic writings, is decathecting internalized parental images. It is possible that father-present females produced significantly more death and loss themes because they were actively coping with their fantasies of parental loss. The significantly fewer fantasies of parental loss given by father-absent females is consistent with this psychoanalytic model which suggests that the loss of a parent at an earlier developmental period complicates the decathexis process in adolescence. Reasons for the failure to find differences between father-absent and father-present males are discussed, and suggestions for future research are provided.This research is based upon the doctoral dissertation of the first author.Received his Ph.D. in clinical psychology from the University of Texas at Austin; internship at the Children's Hospital Medical Center, Boston, Massachusetts. Current research interests include death and dying and adolescent personality development.Received his Ph.D. from the University of Minnesota. Current research interests include imaginary companions and fantasy in young children and adolescent psychosexual development.     

Employees' provident fund organization: Empowering members by digital transformation

Employees' Provident Fund Organization (EPFO) was established by the Government of India with the purpose of ensuring financial and social security for industrial workers and their dependents. With more than 150 million accounts of its members, EPFO is the world's largest social security organization. Operational processes of EPFO were riddled with over emphasis on rules and regulations but were weak on transparency, accountability, effectiveness, and efficiency. Consequently, the primary purpose of social security of industrial employees was compromised. Business process reengineering combined with adoption of Information and Communication Technology (ICT) and social media brought a sea change to the functioning of EPFO. This paper documents the transformation of EPFO from a bureaucratic, opaque organization to a customer-centric, stakeholder-friendly, transparent, and accountable organization.     

Does Distance Matter Morally to the Duty to Rescue

Law and Philosophy -