The X-chromosome has valuable characteristics for population genetic studies. In order to investigate the genetics of the human Mediterranean populations further, we developed a 25 X-chromosome SNP-multiplex typing system. The system was based on PCR multiplex amplification and subsequent multiplex single base extension with the SNaPshot reaction, capillary electrophoresis and multicolor fluorescence detection.We investigated 11 Mediterranean populations with the 25 X-chromosome SNPs. A high overall homogeneity was found among the Mediterranean populations except for Moroccans, who differed genetically from the rest of the populations in the Mediterranean area. This result supports the hypothesis of a low incidence of the south-north genetic interchange at the western shores of the Mediterranean basin. A low genetic distance was found between populations in the Middle East and the western part of the Mediterranean area most likely reflecting the strong effect of the Neolithic wave.A certain level of background linkage disequilibrium among the 25 SNPs on the X-chromosome in Ibiza and Cosenza was observed, possibly as a consequence of their demographic history. 相似文献
We constructed a simple STR pentaplex of new loci recommended as next generation markers for the European Standard Set (ESS) comprising normal-amplicon STRs: D12S391 and D1S1656, plus mini-amplicon STRs: D2S441, D10S1248 and D22S1045. Validation of the pentaplex included evaluation of its ability to amplify DNA from a variety of degraded forensic casework samples. Although the ESS-pentaplex was designed in the first instance to generate allele frequency data to supplement existing databases of established STRs, the multiplex proved to be a valuable tool for the analysis of challenging DNA when certain markers of Identifiler or MiniFiler occasionally failed. 相似文献
Researchers have not investigated mental health outcomes among couples who are reciprocally violent towards each other. The
present study investigated differences in partner violence (psychological, physical, and sexual) and mental health symptoms
(depression, anxiety, hostility, and somatic) between two types of reciprocally violent couples: situational couple violence
(SCV) and mutual control violence (MVC). SCV couples use violence to address stressful family conflicts, while MVC couples
use violence as a tool to control each other. Participants (N = 609) completed surveys that contained several instruments that measured past violence, coercive behaviors, physical injuries,
and mental health symptoms. Results revealed that MVC reported significantly higher levels of violent perpetration and worse
physical and mental health than SCV. These findings have implications for understanding the role of coercion in partner violence
and mental health, which can be used for the development of appropriate mental health services for couples who are mutually
violent towards each other. 相似文献
Abstract: Effectively addressing marijuana trade is aided by understanding marijuana geographic sources. We analyzed the 87Sr/86Sr of marijuana samples grown in 79 counties across the United States to determine if a primary geologic signal is retained in marijuana, which could therefore be useful for geographic sourcing. The marijuana results were compared with modeled bedrock 87Sr/86Sr values based on 87Rb decay rates and a generalized geologic map of the U.S.A. A significant correlation was observed between marijuana 87Sr/86Sr and modeled bedrock 87Sr/86Sr. Although values clustered near the 1:1 relationship, there was a predominance of positive anomalies, perhaps attributable to carbonate bedrock. A small number of negative anomalies were also observed, which were generally associated with granitic bedrocks. These results suggest that strontium isotopes in marijuana record the geographic origins of marijuana, and that refinement of the base strontium map (or strontium isoscape) and improved understanding of other strontium sources would be productive. 相似文献
This paper analyses the allocation of R&D investments within rare diseases and identifies the characteristics of rare diseases that appear to lead R&D resources. Rare diseases affect less than 1 in 2000 citizens. With over 7000 recognised rare diseases and 350 million people affected worldwide, rare diseases are not so rare when considered collectively. Rare diseases are generally underserved by drug development because pharmaceutical industries consider R&D investments in rare diseases too costly and risky in comparison with the low expected returns due to the small population involved. We use data on rare diseases research from Orphanet along with academic publications per rare disease from bibliographic databases. We test the existence of inequalities in R&D investments within rare diseases and identify the disease characteristics that appear to lead R&D investments using dominance tools and bilateral tests. We show that rare diseases in children and with a smaller prevalence, such as ultra-rare diseases, are underserved by R&D. R&D investments appear to be concentrated in more profitable research areas with potentially larger sample size and adult population.
In criminal activities, soil can be transferred from a crime scene to items linked with a perpetrator; for example, shoes, cars or tools. Several parameters will influence the quantity of soil transferred in a given scenario. The knowledge of the most influential factors can help the expert to assess the evidence using a logical approach at the activity level or to predict the amount of soil that can be expected in a given scenario. The influence of five chosen parameters, namely the shoe profile, shoe size, walker's weight, soil type and soil humidity were assessed using Design of Experiment (DOE) in order to understand their influence on soil quantity transferred on shoes.The Faced Central Composite Design (FCCD) using a quadratic model was found to be highly significant, thus they could be adequately used to model and to interpret the amount of soil recovered from one shoe.These designs demonstrate that the characteristics of the donor (soil type and soil humidity), as well as a combination of these two factors have a very significant impact on the soil transfer. The characteristics of the receptor (shoe profile, shoe size and walker's weight) also have an impact on the transfer but to a lesser extent.Globally, this research provides valuable information for the forensic scientist both in investigative mode: evaluation of the soil quantity possibly transferred on shoes, and in the evaluative steps: is the quantity of soil found on the suspect shoes in accordance to the proposition/scenario given by the prosecution and the defence? 相似文献
The international fight against money laundering illustrates changes in global governance as a result of the increasingly cross-border nature of crime and the need it creates for all involved to cooperate. The economic priorities and security concerns that surround it contributed to the strong evolution of global governance in this area and the status of anti-money laundering as a shared problem. The creation of the Financial Action Task Force (FATF), its expansion and cementation throughout the years, is a good example of the many forces working together to responding to the demands of emerging criminal threats and trends. It offers a good illustration of how relationships in global governance have influenced FATF’s priorities and action and ultimately the way in which illicit financial flows are tackled. This analysis offers an overview of FATF’s network across time taking into account the role of states, international organisations, and the private sector in the decision-making processes. It argues that Great Powers – a small, but aligned, group of states of global economic relevance – are responsible for FATF’s direction and the international efforts against illicit financial flows. It suggests, however, that unlike what could be expected, their power is declining following the rise of private sector influence through resourceful, organised and transnational actions e.g. on information sharing. 相似文献