Age Estimation Based on DNA Methylation Using Blood Samples From Deceased Individuals

Age estimation using DNA methylation levels has been widely investigated in recent years because of its potential application in forensic genetics. The main aim of this study was to develop an age predictor model (APM) for blood samples of deceased individuals based in five age-correlated genes. Fifty-one samples were analyzed through the bisulfite polymerase chain reaction (PCR) sequencing method for DNA methylation evaluation in genes ELOVL2, FHL2, EDARADD, PDE4C, and C1orf132. Linear regression was used to analyze relationships between methylation levels and age. The model using the highest age-correlated CpG from each locus revealed a correlation coefficient of 0.888, explaining 76.3% of age variation, with a mean absolute deviation from the chronological age (MAD) of 6.08 years. The model was validated in an independent test set of 19 samples producing a MAD of 8.84 years. The developed APM seems to be informative and could have potential application in forensic analysis.     

Transplantation of organs and tissues and some reflections on the "solidarity" of the human cadaver in Portugal

The article presents the legal situation of transplantation medicine in Portugal and presents some interesting statistical data. The author defends the opting-out system and presents an argument in favour of such solution, taking into account the mandatory nature of forensic autopsies.     

Tests of one Brazilian facial reconstruction method using three soft tissue depth sets and familiar assessors

Facial reconstruction is a method that seeks to recreate a person's facial appearance from his/her skull. This technique can be the last resource used in a forensic investigation, when identification techniques such as DNA analysis, dental records, fingerprints and radiographic comparison cannot be used to identify a body or skeletal remains. To perform facial reconstruction, the data of facial soft tissue thickness are necessary. Scientific literature has described differences in the thickness of facial soft tissue between ethnic groups. There are different databases of soft tissue thickness published in the scientific literature. There are no literature records of facial reconstruction works carried out with data of soft tissues obtained from samples of Brazilian subjects. There are also no reports of digital forensic facial reconstruction performed in Brazil. There are two databases of soft tissue thickness published for the Brazilian population: one obtained from measurements performed in fresh cadavers (fresh cadavers' pattern), and another from measurements using magnetic resonance imaging (Magnetic Resonance pattern). This study aims to perform three different characterized digital forensic facial reconstructions (with hair, eyelashes and eyebrows) of a Brazilian subject (based on an international pattern and two Brazilian patterns for soft facial tissue thickness), and evaluate the digital forensic facial reconstructions comparing them to photos of the individual and other nine subjects. The DICOM data of the Computed Tomography (CT) donated by a volunteer were converted into stereolitography (STL) files and used for the creation of the digital facial reconstructions. Once the three reconstructions were performed, they were compared to photographs of the subject who had the face reconstructed and nine other subjects. Thirty examiners participated in this recognition process. The target subject was recognized by 26.67% of the examiners in the reconstruction performed with the Brazilian Magnetic Resonance Pattern, 23.33% in the reconstruction performed with the Brazilian Fresh Cadavers Pattern and 20.00% in the reconstruction performed with the International Pattern, in which the target-subject was the most recognized subject in the first two patterns. The rate of correct recognitions of the target subject indicate that the digital forensic facial reconstruction, conducted with parameters used in this study, may be a useful tool.     

Political Institutions,Policymaking, and Policy Stability in Latin America

In some Latin American nations, policy change occurs frequently, while in others it is stable, less prone to shifts with the prevailing political climate or shocks. The conditions under which institutional rules and the powers of key actors influence the capacity for governance vary, and this variation is seldom addressed in the literature. This project examines the effects of the interactions between key policymakers (the executive and the legislature) in Latin America on policy stability across different institutional frameworks. Countries with simultaneously strong executives and weak legislatures are shown to have unstable policy environments, as are countries with a history of unified government and, to a lesser extent, candidate‐centered electoral systems.     

Diel Activity and Effect of Carcass Decomposition on the Attractiveness to the Forensically Important Species Oxelytrum Discicolle (Coleoptera: Silphidae)†

Oxelytrum discicolle is a carrion beetle commonly found in the Neotropical region, mainly associated with cadavers and carcasses. Information on behavior related to the likelihood of cadaver colonization is scarce. We performed two field experiments in peri‐urban forest in Brazil in order to strengthen our knowledge on O. discicolle behavior. In the first experiment, we assessed the preference for piglet carcasses at different stages of decomposition offered simultaneously in two seasons, dry and rainy, while in the second experiment we investigated the diel activity of adults. A total of 493 adults were collected in the experiments. A significantly higher abundance was detected in the rainy season. There was a marked preference for carcasses at advanced stages. Adults exhibited a typical nocturnal behavior, the number trapped at night was 17 times greater than those collected during the day. Oxelytrum discicolle is nocturnal species that prefers carcasses in advanced stage of decomposition.     

Standardisation of nomenclature for dog mtDNA D-loop: a prerequisite for launching a Canis familiaris database

Domestic dogs are increasingly involved, often as protagonists, in the forensic scene. Acknowledging this fact and benefiting from the accumulated experience on human mitochondrial DNA (mtDNA) analyses, we propose a standard for Canis familiaris mtDNA sequences as a prerequisite for the launching of the corresponding database.     

Leave them kids alone! National exams as a political tool

Public Choice - In this paper, we show how results in state administered university entrance standardized exams in Portugal are sensitive to the electoral cycle. Using individual data for all...     

Parliamentary Questions as an Instrument for Geographic Representation: The Hard Case of Portugal

This article asks whether and why, in a system lacking electoral incentives to cultivate personal votes, MPs might choose to signal to geographic constituents. It explores this question by analysing the number of written parliamentary questions submitted to the Portuguese parliament on two issues – unemployment and crime – between 2009 and 2015, and asking if MPs are more inclined to table questions on specific issues when their districts suffer particularly from related problems. The article finds evidence that constituency‐level problem pressure does matter for the signalling activities of MPs, although policy specialization remains the main driver of their issue emphasis. This finding contributes new knowledge to the ongoing debate on the factors accounting for the representative relationship between MPs and constituents, by drawing attention to the importance of district‐level problem pressure as one of the drivers of issue sponsorship in parliament.     

Perceptions and personal experiences of unwanted attention among Portuguese male students

The present study investigated male perceptions and personal experiences of ‘unwanted attention’ (UA), as well as possible associations between perceptions and personal experiences of UA. Ninety-one male college students, from five Portuguese universities, were asked to indicate which of a continuum of 47 behaviours represented UA. Although UA, stalking and harassment are rarely addressed in Portugal, male college students shared a clear understanding of what behaviours constituted UA, with the identification of four main categories of UA behaviours: ‘aggressive’, ‘threatening’, ‘classic’ and ‘dysfunctional attachment’. Almost all participants (96%) reported personal experiences of at least one UA behaviour. There was a minimal relationship between perceptions and personal experiences of the individual behaviours. The findings highlight the widespread risk of male victimisation and the need to legitimise male complaints.