Numerical density of delta-opioid receptor expressing neurons in the frontal cortex of drug-related fatalities

In animal experiment and in cell culture, chronic morphine treatment has been followed by a reduction as well as an increase of the delta-opioid receptor (OR) number. The present postmortem morphometric study of morphine-related fatalities of drug addicts (n=12, 22-35 years old, with blood unconjugated morphine levels from 27.1 to 407 ng/ml, m.v. 176.9 ng/ml) versus a non-addicted control group (n=13, 10-44 years old) is intended to examine whether chronic opiate exposure also affects the numerical density of deltaOR expressing neurons in the human neocortex (area 10 according to Brodmann (Vergleichende Lokalisationslehre der Grosshirnrinde (1909) Johann Ambrosius Barth, Leipzig)). For the immunohistochemical procedure, vibratome sections (100 microm) were incubated with a monoclonal antibody against the deltaOR diluted 1:100, and immunoreactive sites were visualized using an immunoperoxidase protocol. The numerical densities of OR expressing and Nissl-stained neurons were assessed morphometrically (camera lucida drawings). In both collectives, the anti deltaOR immunoreactivity was predominantly localized in pyramidal neurons of layers (L) II/III and V as well as in round and ovoid neurons of L VI. In the drug-related fatalities, the density of neurons expressing deltaOR protein amounted for 2515+/-240/mm(3), in the control group for 2616+/-204/mm(3), thus displaying no statistically significant difference. These findings go along with the binding behavior of opioid ligands in postmortem brains of heroin addicts revealing similar receptor densities and affinities in the control subjects and addicts.     

Shi’i division over the Iraqi state: decentralization and the Islamic Supreme Council of Iraq

ABSTRACT

This article traces the evolving political platform of one of Iraq’s oldest and most powerful Shi’i political parties, the Islamic Supreme Council of Iraq (ISCI). Drawing on an analysis of 15 years of primary materials produced by ISCI, it focuses principally on their promotion of decentralization as a path towards peace and stability in Iraq. However, the article also traces the origins of a deep schism that emerged within ISCI between the movement’s old guard who were beholden to the Iranian regime and their model of vilāyat-i faqīh, and the youth-led Iraqi nationalist faction who wanted to see the instalment of a civil government without religious oversight. The article demonstrates that this division is indicative of a theological debate between Shi’i religious scholars over differing interpretations of the role of Shi’ism in politics. The article concludes by arguing that understanding the extent to which such esoteric religious debates manifest themselves politically is crucial to interpreting divisions within Shi’ism not just in Iraq, but across the broader Middle East.     

Technology transfer at land-grant universities

Improvement in the ability of universities in the United States to transfer technology to the private sector is seen as a factor in strengthening American competitiveness. To better understand the university's role in this process, a survey of formal university programs in technology transfer was performed through personal interviews at four midwestern land-grant universities. There are six basic types of programs in place: technical assistance, affiliate, licensing, business development, incubators and research parks, and information networks. For purposes of classifying these programs, we used the model developed by Janis et al. (1) It was found that, of the six modes of technology transfer, two could be considered active, three semi-active and one passive.     

Cardiac glycosides and metabolites--problems of recovery in tissue extracts. Separation of visible substance spots in the nanogram range (author's transl)]

The recovery measurements in rat tissues performed via i.p. injected radioactive digoxin derivates (3H-digoxin, 125J-digoxin derivative) showed that approximately 50% of the total glycoside content will be extracted. Thus, an addition of digoxin standards to drug-free tissues may lead to false negative determinations. By comparison of the radioactivity before and after extraction the following results were obtained: Recovery from tissues 3H-digoxin 50% 125J-digoxin 40% from serum 3H-digoxin 60% added to drug free tissue homogenates 3H-digoxin 85% After i.p. application of 15 mg/kg of beta-methyldigoxin to BD9 (Berlin)-rats the resulting tissue concentrations were extracted by Amberlite XAD-2. beta-Methyldigoxin and its metabolites digoxin and digoxinbisdigitoxide could be separated and distinguished from artifacts by fluorescence detection on HPTLC-plates with a detection limit of 60 ng/spot. Concentration determined by radioimmunoassay are in satisfactory agreement with HPTLC results.     

The effect of a Charter of Rights on the policy-making role of Canadian courts

Abstract This paper explores the thesis that the main impact of the new constitutional Charter of Rights on the Canadian system of government is to increase the role of the judiciary in deciding how fundamental social and political values should be affected by public policy. The first part of the paper. by reviewing the significant role which trial and appeal courts already play in shaping public policy, aims at overcoming a tendency to exaggerate the change in our system of government resulting from the Charter. The second part identifies the factors which will influence the judicial response to the Charter and the policy areas in relation to which judicial interpretation will be most significant. The analysis shows that the Charter's major impacts will likely include much greater judicial supervision of police activity and centralization of policy-making under the leadership of the Supreme Court on questions of social justice and minority-language rights. It concludes that, while the Charter will increase Canadians' understanding of judicial policy-making, it represents a retreat from reliance on the political process to resolve divisive social issues. Sommaire. Le présent article examine la thèse selon laquelle le principal effet de la nouvelle Charte des droits constitutionnels sur le système de gouvemement canadien sera d'accroître le rôle du pouvoir judiciaire dans la détermination de la façon dont les valeurs sociales et politiques fondamentales devraient être affectées par les politiques publiques. La première partie de l'article passe en revue Ie rôle important que jouent déjà les tribunaux et les cours d'appel, ainsi que l'influence que ces institutions exercent sur la politique publique, ceci afin de neutraliser une tendance à exagérer les changements qu'apporterait la charte à notre système de gouvernement. La seconde partie identifie les facteurs qui pourront influencer la réponse judiciairs à la charte ainsi que les domaines politiques où l'interprétation judiciaire de cette charte aura le plus d'importance. L'analyse démontre que l'adoption de la charte entraînera des conséquences importantes, qui comprendront probablement une surveillance judiciaire des activités de la police beaucoup plus développée et une centralisation, sous la direction de la Cour suprême, de l'élaboration de politiques en matière de justice sociale et de droits linguistiques des minorités. L'auteur conclut que bien que la charte permettra aux Canadiens de mieux saisir le rôle de la Cour dans l'élaboration des politiques, elle représente un recul du recours au processus politique en ce qui concerne la résolution des questions sociales contentieuses.