Development and usage of a NIST standard reference material for real time PCR quantitation of human DNA

National Institute of Standards and Technology SRM 2372 human DNA quantitation standard has been produced to support the need for a human-specific DNA quantitation standard in forensic casework and calibration of new quantitative polymerase chain reaction (qPCR) assays. The conventional DNA concentration has been assigned with one of the U.S. National Reference UV/Visible Spectrophotometers, assuming an absorbance of 1.0 at 260 nm equals 50 ng/μL of double stranded DNA. In addition, an interlaboratory study has been conducted, to verify that the SRM 2372 materials perform well in currently used DNA quantitation assays by the forensic DNA community. Each unit of SRM 2372 consists of three well-characterized DNA extracts. Component A is a single-source human male material derived from blood. Component B is a multiple-source human female material derived from blood. Component C was purchased as a purified unsheared genomic human DNA (Sigma-Aldrich Co., St. Louis, MO) obtained as a lyophilized human genomic extract and has both male and female donors. SRM 2372 is intended to enable the comparison of DNA concentration measurements across time and place. Manufacturers can use SRM 2372 to validate the values assigned to their own reference materials. Individual forensic laboratories can use SRM 2372 to validate DNA quantitation methods and to verify the assigned concentration of in-house or commercial DNA calibration standards.     

Zuständigkeit für Klage auf Aufteilung einer Nachlasssache

Die Klage auf Aufhebung der Miteigentumsgemeinschaft an einer einzelnen Nachlasssache kann Erbteilungsklage iSv § 77 Abs 2 JN sein.     

The contradictions of class and ideology among argentine laborers and employees

This article compares Argentine trade union laborers' and employees' notions of class and ideology. The research findings were based on one-to-one structured, but openended interviews with 110 rank and file workers of Greater Buenos Aires between 1985 and 1986. Rather than a study of union structure or labor leadership, it fleshes out workers' views beyond the factory walls and office windows. The research depicts the complex, seemingly contradictory, variety of social-democratic, liberal, and conservative values and attitudes carried by the Argentine working class. Peronism remains the ideological anchor of many workers who, nevertheless, consider themselves centrist, left or right. They are centrist in their support of a liberal-capitalist economy, left in their espousal of markedly better income distribution for workers, and right in the defense of their communities and neighborhoods. Workers' historical connections, cultural outlooks, and educational levels impact heavily upon their views of democracy and sense of class. Peter Ranis is a professor of political science at York College and the Graduate School and University Center, City University of New York. He has completedArgentine Workers: Peronism and Class Consciousness, to be published by the University of Pittsburgh Press in 1992. The research for this article was made possible by a Fulbright Senior Research Grant and several PSC/ CUNY awards between 1986 and 1990. My thanks also go to the Instituto Torcuato Di Tella of Buenos Aries and the Centro de Investigaciones Sociales Sabre el Estado y la Administracíon (CISEA) for respectively making their facilities available to me in July and August of 1987 and 1990.     

An investigation into the use of calculating the first derivative of absorbance spectra as a tool for forensic fibre analysis.

A range of fibre samples was measured using J&M MSP400 and J&M MSP800 microspectrophotometers across the visible and UV/visible wavelength ranges respectively. The first derivative of the absorbance spectra was then calculated and studied. When the absorbance spectra produced for some samples were broad and featureless, the first derivative spectra provided more points of comparison that facilitated discrimination. For many of the samples, calculating the first derivative did not result in any additional discrimination due to the high number of points of comparison present in the absorbance spectra. However, for the samples that exhibited a high level of intra-sample colour variation (e.g. through uneven dye uptake common in cotton and wool, etc.), which was evident in the absorbance spectra, the associated first derivative spectra highlighted this variation between the fibres and could potentially have resulted in false exclusions. The results show that whilst calculating first derivative can be a useful aid in the comparison of spectra, a high degree of caution is required when applying this method to fibres which exhibit a large intra-sample variation in colour.