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831.
探寻FOS蛋白在心肌早期缺血再灌流损伤中变化规律,为心性猝死的诊断提供新方法。利用SD大鼠建立心肌缺血再灌流损伤模型,设立正常、缺血对照组与缺血再灌组。心脏标本经HE染色及免疫级化观察。结果发现,在冷冻切片上缺血20min再灌流30min,再灌流区心肌细胞核呈阳性着色。但在石蜡切片上,缺血30min再灌流30min后,再灌流区才有心肌细胞核(37.76%±9.66%)呈弱阳性着色,再灌流60min后核呈棕褐色阳性染色,120min后开始减弱(35.36%±3.16%)。正常和单纯缺血组心肌细胞核未见有阳性反应。HE染色无明显病理改变。结果提示,SABC-FOS免疫组织化学方法最早可揭示心肌缺血20min再灌流30min的损伤,FOS蛋白在再灌流后60-120min之间可能有一个高峰表达。此法对显示实验性心肌早期缺血再灌流损伤有重要的价值。有望用于心性猝死的诊断。 相似文献
832.
For postmortem diagnosis of viral myocarditis, 12 specimens of heart in autopsy were studied immunohistochemically with anti-fibronectin(FN) antibody. Among 5 cases with definite or suspected myocarditis, intensive FN-positive could be found in all monocytes, macrophages and some neutrophils and there were 3 cases with FN-positive cardiomyocytes. While in 3 violent death cases with leucocytes infiltration in the interstitial tissue of myocardia, no FN-positive cardiomocytes were observed, which was the same as that of 4 case without cardiac pathological changes. Moreover, there were rarely FN-positive leucocytes in the 7 cases without viral myocarditis. The results suggest that the immunohistochemical observation of FN in myocardia might be of value for detecting slight degeneration of cardiomyocytes and determining the inflammatory leucocyte infiltration in myocarditis. 相似文献
833.
A solid-phase extraction method of morphine from urine and blood has introduced. The effect of 5 SPE columns, 3 eluents and pH on morphine recovery has been investigated systematically. Derivative GC was used as a method of detection. The result showed that the column and the eluent of such as GDX-301, GDX-403 and C18 chloroform:isopropanol (9:1) had good behaviors to extraction of morphine. When GDX-301 was used as a sorbent, the recovery of morphine from urine was above 90% at pH 9, then went down with the increase of pH. While the recovery from blood was growing with the increase of pH, which reached above 90% in strong alkaline. The extraction method is simple, inexpensive, efficient and reproducible, which provides an effective and practical method to extract morphine and similar illicit drugs from biological fluids. 相似文献
834.
835.
通过两例肾上腺结核猝死的案例观察发现:肾上腺和肾上腺以外器官的结核病变均表现为不典型的慢性陈旧性的病理学特征。提示在法医学鉴定中,对患过结核病和“不明死因”猝死尸体剖验时肾上腺的组织病理学检验尤为重要 相似文献
836.
837.
Dwight Y. King 《公共行政管理与发展》1988,8(3):249-260
Decentralization of a substantial portion of development activity under Indonesia's New Order has been attempted in the context of centralizing civil service reforms. This analysis, based on field observation in two areas distant from the ‘bureaucratic centre’ of Java, South Sulawesi and East Nusa Tenggara in early 1986, shows some ways in which the structure of the civil service and recent policies governing it have handicapped regional governments in their attempts to develop an apparatus capable of managing decentralization. For example, the structure often leads local officials to prefer rank over technical qualifications in appointments to managerial positions. The analysis also shows how they cope and attempt to maintain their legal prerogatives vis-à-vis the central government. In conclusion policy recommendations are offered that would help to achieve a better balance between the New Order's concerns for centralization and decentralization. 相似文献
838.
The purpose of this study is to estimate the age of cadavers by histomorphometry of the femur. Seventy-two Japanese males ranged from 43 days to 92 years old and 26 females ranged from 2 to 88 years old were used. The thickness of sections was adjusted at 50 to 70 microns by grinding with sand paper. The sections were not decalcificated. They were stained with Villanueva's bone staining powder and with thionin dye. Microradiographs of the sections were obtained by the soft X-ray apparatus. The area, maximum and minimum diameter, and perimeter of the perfect osteon and Haversian canal were measured. In addition, the type II osteon number, osteon fragment number, and area of triangle were also determined. All these parameters were examined by an image analyzer. The parameters of the osteon showed high correlation coefficient with age (magnitude of r > 0.77), while those of the Haversian canal were low (magnitude of r < 0.11). All parameters were subjected to multiple regression analysis for producing a multiple regression equation of age estimation. For the stepwise selecting method, the perimeter of osteon, maximum length of the Haversian canal and osteon fragment number were selected for the equation. Their multiple r2 and standard error of estimation were 0.8874 and 6.39, respectively. For the forward selection method, in addition to the above items, three parameters, the maximum length of Haversian canal, triangle area, osteon fragment number were selected. Their multiple r2 and standard error of estimation were 0.9484 and 4.884, respectively. Bone staining was useful to clarify the demarcation between osteon and fragment, leading to an increase in the accuracy of age estimation. However, the entire range from birth to 90 years was difficult to cover for precise age estimation. 相似文献
839.
840.
医疗纠纷的法医临床学鉴定 总被引:2,自引:2,他引:0
目的:剖析医疗纠纷产生的原因;介绍法医介入鉴定的法律依据,法医学鉴定的特点,赔偿的方法,存在的问题等;在最终减少案件发生的前提下,妥善地处理此类诉讼案件。方法:对46例医疗纠纷案件进行回顾性研究,将其按案件的性质、涉及的器官、引起的后果等归类。分析。结果:80%的案例其医疗行为与最终的不良后果之间存在着因果关系,原因包括手术医师对手术区域局部解剖结构分辨不清等技术性因素以及医护人员粗心大意、责任心不强等责任性因素;20%无因果关系。结论:临床医师应与其他行业一样为其所实施的医疗行为承担一定的法律责任。医疗纠纷作为一种特殊的民事侵权行为,在进行民事诉讼赔偿时,法医有必要对其中的因果关系进行评定,以保护双方当事人的合法权益。 相似文献