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841.
This article describes the application of solid-phase microextraction (SPME) to the recovery of manufacturing by-products and impurities from an illicit drug seizure. The preparation chosen for examination using this technique contained 4-methoxyamphetamine, an hallucinogenic amphetamine that has been encountered frequently in South Australia. Compounds found in the PMA preparation included 4-methoxyphenol, 4-methoxybenzaldehyde, 4-methoxyphenyl-2-propanone, 4-methoxyphenyl-2-propanol, 4-methoxyphenyl-propene, and (tentatively) 4-methyl-5-(4'-methoxyphenyl) pyrimidine. The presence of these compounds suggests that the active drug was prepared from 4-methoxybenzaldehyde via 4-methoxyphenyl-2-propanone using a Leuckardt reductive amination. In this instance, SPME was found to be a simple, rapid, and non-destructive recovery technique that gave results complementary to those provided by conventional liquid-liquid extraction. There is an indication that SPME might find application in profiling of illicit drugs. 相似文献
842.
A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework. 总被引:5,自引:0,他引:5
T D Anderson J P Ross R K Roby D A Lee M M Holland 《Journal of forensic sciences》1999,44(5):1053-1056
A validation study was conducted to demonstrate that deoxyribonucleic acid (DNA) could be successfully extracted from human nail material and analyzed using short tandem repeat (STR) profiling and/or mitochondrial DNA (mtDNA) sequencing. This study involved the development of a DNA extraction protocol that includes a cleaning procedure designed to remove external contaminants (e.g., biological, chemical). This protocol was used to test human nail material that had been soaked in whole blood from a second donor and coated with gold-palladium to simulate scanning electron microscopic analysis. The results showed no indication of a mixture and were consistent with that of the nail donor. Fresh human nail material usually yielded both STR profiles and mtDNA sequence information; however, aged human nail material (approximately eight years old) yielded only mtDNA sequence information. Upon completion of the validation study, the extraction protocol was used for the analysis of a torn fingernail fragment recovered from the scene of a violent homicide in 1983. A partial STR profile and mtDNA sequence information indicated that the fingernail fragment was excluded as originating from the suspect and was, in fact, consistent with originating from one of the victims. 相似文献
843.
Chorionic villus sampling prior to pregnancy termination, a tool for forensic paternity testing. 总被引:1,自引:0,他引:1
A Reshef P Brauner M Shpitzen N Gallili A Marbach U Motro E Shmueli V Meiner 《Journal of forensic sciences》1999,44(5):1065-1068
Chorionic villus sampling (CVS), prior to pregnancy termination (pre-termination CVS), is suggested as a tool for forensic paternity testing. Unlike the abortion material, which consists of ruptured tissues of fetal and maternal origin, extra-embryonic membranes obtained through CVS can provide an uncontaminated source of fetal tissue for genotyping. We discuss the possibility of confined placental mosaicism (CPM) and its implications on the polymerase chain reaction (PCR) based analyses of short tandem repeats (STRs) and the D1S80 loci. 相似文献
844.
Accidental hypothermia has been described in the forensic literature but reports of occurrence in hospitalized patients are rare. Associated anatomic lesions include acute hemorrhagic pancreatitis and characteristic acute gastric ulcers termed Wischnewski ulcers. We report here two patients with cirrhosis and ascites; one also had hepatocellular carcinoma. Portal vein thrombosis, acute hemorrhagic pancreatitis and Wischnewski ulcers were present in both. The clinical records documented hypothermia that progressed over several days. Temperature nadirs of 31.0 degrees C (87.8 degrees F) and 32.2 degrees C (90.0 degrees F) were recorded in each patient, respectively, one day before death, although each transiently reached temperatures that did not register on standard monitoring devices. This is the first report that chronicles antemortem body temperatures in hypothermic patients with Wischnewski ulcers and pancreatitis at autopsy. Also, the association of these findings with portal vein thrombosis and cirrhosis has not been previously described. We discuss this constellation of findings with regard to possible mechanistic interrelations. 相似文献
845.
R V Babakhanian G V Ivanova T A Kostyrko A E Safra? O D Iagmurov 《Sudebno-meditsinskaia ekspertiza》1999,42(3):6-9
Histological analysis of the viscera in experimental poisoning with psilocybin-containing mushrooms showed nonspecific changes in all examined organs, presenting as expressed hemocirculatory disorders and intracellular dystrophy. Quantitative histochemical analysis showed appreciable shifts in the activities of enzymes involved in the cytoplasmic and mitochondrial redox processes and of specific enzymes involved in nerve tissue metabolism. This may reflect some features in the direct effects of narcotic alkaloids contained in psilocybin-producing mushrooms. 相似文献
846.
V D Iablochkin 《Sudebno-meditsinskaia ekspertiza》1999,42(4):30-31
Acetylene traces were detected by gas chromatography in the cadaveric right crural muscle of a 30-year-old man dead from an explosion of an acetylene reservoir at a plant. Acetylene was identified using the absolute calibration method on 3 standard gas chromatographic columns, reaction gas chromatography, and acetylene "deduction" by silver sulfate on silicagel. 相似文献
847.
The index of lethal poisoning with ethyl alcohol and its surrogates in Russia remains one of the highest in the world. It is explained by high alcohol consumption in Russia (about 15 liters of pure alcohol per capita annually), consumption of mainly strong beverages (about 80%), extensive sales of adulterated strong alcoholic beverages (more than 25%), virtually no preventive work in the population, and other causes. The index of lethal poisoning with alcohol and its surrogates in the country and its regions is one of the most important parameters in evaluation of the socioeconomic well-being of the population. 相似文献
848.
Macro- and trace element composition of hairs on the head, chest, in the armpits, and on the pubis in the population of Asia, Africa, and Latin America adapted and not to the climate, geographic and ecological conditions of the Russian Federation (Moscow) is studied by spectrography (DPS-13 spectrograph). Element composition of residents of 16 countries is analyzed by variation statistics methods using PC: Lebanon, Syria, Yemen, Bahrain, India, Sierra Leone, C?te d'Ivoire, Rwanda, Kenya, Sudan, Mexico, Ecuador, Bolivia, Brasilia, Columbia, and Chile. Hairs from residents of East Europe (Russians, Byelorussians, Ukrainians) living in Moscow for 6-8 years (adapted) were the reference for comparison. A total of 371 specimens of hairs were examined. Hairs from adapted and not adapted residents of the above-listed countries and continents differ significantly from each other and from the hairs of Russians, Byelorussians, and Ukrainians living in Moscow. 相似文献
849.
850.