A new individualization marker of semen: deoxyribonuclease I (DNase I) polymorphism.

We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen.     

Children in poverty: A committee prepares for an informed debate

By gathering and disseminating information, members of the Committee on Ways and Means, and committee staff under their supervision, have played an important role in informing the debate on poverty issues during the 1980s. In the face of President Reagan's 1981 budget program, which reduced spending in the major antipoverty programs, committee members targeted substantial resources to an ongoing, albeit ad hoc, education process. The process has not only provided evidence of the adverse impact of the 1981 reductions on the poor, but has also made new contributions to the understanding of poverty. Although many other factors have contributed to outcomes in poverty policy during the 1980s, it is clear that this education process played an important role in the successes achieved thus far; partial reversal of the 1981 reductions has been achieved, tax changes have been implemented which help low-income families, and a welfare reform bill has been enacted.     

Perceptions of repressed memories: A reappraisal

College students read a trial summary of a sexual abuse case. The victim in the case either claimed that (a) her memory for the abuse had been repressed for 20 years and only recently recovered during therapy, or (b) she consciously remembered the abuse for 20 years but never discussed it until recently in therapy. Participants were significantly more likely to convict the defendant when the testimony was described as nonrepressed (67%) versus repressed (58%). This effect was not modified by the age of the victim at the time the alleged abuse occurred (either 3, 8, or 13 years of age), although the younger and older victims were significantly less believable than the 8-year-old victim. Compared to female participants, male participants were significantly less likely to convict the defendant and rated the victim as significantly less believable. These findings are discussed in the context of recent research on juror reactions to repressed memory testimony.     

Gc subtyping in blood stains by isoelectric focusing in immobilized pH gradients

The applicability of isoelectric focusing in immobilized pH gradients for the Gc-subtyping in the forensic examination of bloodstains was tested. It is shown that due to the excellent separation of the Gc 1S and 1F bands subtyping of bloodstains can be done with high reliability.     

Causes of death of patients in an institution for the developmentally disabled

The causes of death of 53 severely to profoundly developmentally disabled patients who died in an intermediate care facility were reviewed. Respiratory disease, predominantly pneumonia and aspiration, accounted for 72% of deaths. Seven patients died of nonrespiratory causes, and in 8 patients, no cause of death could be determined, even after a complete autopsy or investigation. The median age at death was 20 years. The weights of these patients' organs at autopsy were lower than those for normal individuals of the same age. The lifespan of these severely impaired individuals continues to be significantly shortened, even with improved methods of care.     

Sudden death in a neonate as a result of herpes simplex infection

This paper describes a case of a neonate with disseminated herpes simplex born to a 14-year-old asymptomatic mother. The infant's physical examination was normal at birth, and subsequent abnormalities were so subtle that infection was not recognized during life. Postmortem cultures of liver and spleen grew herpes simplex virus, and immunofluorescent direct antibody typing revealed Type 2. A cervical culture of the mother obtained after the infant's death was negative.