A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples

The recent formation of a United Kingdom and Irish working group, the Body Fluids Forum (BFF), highlighted the need to investigate different working practices prior to any inter-laboratory comparison work and identification of best practice. Various dilutions of semen were seeded onto swabs and cloth samples for each BFF member laboratory to test using their standard techniques. The results showed that the detection of acid phosphatase on swabs is best achieved using direct testing rather than on an extract from the swab. Extraction methods for spermatozoa require a balance to be achieved between using a sufficient volume of water to ensure optimal release and minimal volume to ensure a concentrated extract. PSA tests were investigated and found to be more sensitive than Choline. DNA profiles were obtained from samples in which no spermatozoa had been detected during microscopic examination.     

Crimes committed by terrorist groups: Theory,research, and prevention

Statement of Purpose: A decline in state-sponsored terrorism has caused many terrorist organizations to resort to criminal activity as an alternative means of support. This study examines terrorists' involvement in a variety of crimes ranging from motor vehicle violations, immigration fraud, and manufacturing illegal firearms to counterfeiting, armed bank robbery, and smuggling weapons of mass destruction. Special attention is given to transnational organized crime. Crimes are analyzed through the routine activity perspective and social learning theory. These theories draw our attention to the opportunities to commit crime and the criminal skills necessary to turn opportunity into criminality. Through these lenses, the research appraises the “successes” and “failures” of terrorists' engagement in crime. Because “failures” can result from law enforcement efforts to (1) interrupt criminal skill development, and/or (2) remove criminal opportunities via technologies and transportation systems, the research represents a best practices approach to the study and control of terrorism. This project was supported by Grant No. 2003-DT-CX-0002 awarded by the National Institute of Justice, Office of Justice Programs, U.S. Department of Justice. Points of view in this document are those of the author and do not necessarily represent the official position or policies of the U.S. Department of Justice.     

Implementation of a semi-automated processing system for DNA profiling of forensic casework samples

The concept for a semi-automated processing system for DNA analysis of crime scene samples was developed at the Landeskriminalamt Baden-Württemberg (LKA BW) and comprises the extraction of genomic DNA from human cells by ChargeSwitch^® magnetic bead technology (CST), quantification of purified DNA by real-time PCR, amplification of short tandem repeats (STRs) by PCR and DNA fragment length analysis of STRs by capillary electrophoresis. Three liquid handling workstations from Tecan, a real-time PCR device and a 16-channel capillary electrophoresis (CE) system, both from Applied Biosystems (AB), are linked via laboratory data network. Transmission and management of sample and analysis data is enabled by a Laboratory Information and Management System (LIMS). Suitability for a wide range of stain types, early exclusion of DNA-free samples, barcode sample identification and prevention of cross-contaminations guarantee efficiency and high quality standards.     

Barack Obama Meets Celebrity Metaphor

Common knowledge suggests that elections are won or lost based on demographics, finances, and other structural elements. Whether candidates win or lose, however, is a matter of action. Symbolic identification, metaphor, and an unfolding narrative—and how they are managed and interpreted in the flow of events—determine who will emerge victorious from the democratic struggle for power. The McCain campaign’s effort to cast Obama as a celebrity, with the hollow trivialities and self-aggrandizement of Paris Hilton and Britney Spears, proves to be one of the most dangerous episodes for the Obama campaign. In response, the Democrats must adjust the staging of Obama’s Thursday night acceptance speech in Denver as a performance of purpose and gravitas, rather than glitz and adoration.     

Student Success and Failure in a Graduate Criminology/Criminal Justice Program

Despite the burdens that students and institutions assume when underwriting educational careers, very little is known about completion rates, risk factors, and successful strategies of criminology/criminal justice (CCJ) graduate programs. Consequently, the present study tracks 287 master’s and 70 doctoral students who entered the Florida State University CCJ program during the 1991–2000 academic years. Almost two-thirds of this cohort earned an advanced degree. Younger students with higher GRE scores were more likely to complete the master’s program. Successful doctoral degree recipients tended to have lower GRE scores, be non-White, and earn their master’s degrees in-house. In terms of time-to-degree, it took over two years to earn an M.A. and slightly more than six years to negotiate the Ph.D. Implications are explored for the program, the university, and the discipline.