Identification of two fire victims by comparative nuclear DNA typing of skeletal remains and stored umbilical tissues

We describe here our collaborative efforts in identifying 2 fatalities of a fire disaster by using a variety of identification techniques. Postmortem findings in both cases were reinforced using Short Tandem Repeat (STR) DNA technology to establish with a high degree of certainty the identities of 2 child victims. STR markers used in the present study include HUMAMEL, HUMCSFIPO, HUMTHO1, HUMvWA, HUMFES/FPS, HUMF13A01, HUMFOLP23, D8S3O6, HUMFGA, and HUMTPOX. Unambiguous identification was made possible through matching DNA profiles generated from skeletal remains with those from umbilical tissues. These tissues were kept by their mothers in accordance with a Philippine tradition and were submitted for DNA analysis. Of the DNA profiles generated from exhumed bone samples of 21 child victims, comparison with the genetic profiles of children A and B obtained from umbilical tissues showed consistent DNA matches with remains 1756 and 1758, respectively.     

Rethinking Representation from a Communal Perspective

Most foundational theories of congressional representation were developed during an era of less polarized and less partisan politics. These theories viewed the incumbency advantage as buttressed by the fact that some constituents were willing to support legislators from the opposite party because of their “home styles.” But in an era of policy immoderation in Congress, this perspective leads to an assumption that citizens evaluate their members of Congress based on what those legislators do for them individually, rather than what they do for their districts more broadly. In this paper, we ask whether citizens take the interests of their fellow constituents into account when evaluating their members of Congress. Using both survey data and an experiment, we uncover support for the notion that citizens take a more communal view of representation as at least part of their evaluations of their representatives. This suggests individuals may have a more nuanced understanding of representation than purely self-interested approaches tend to assume.     

Population data for Y-chromosome haplotypes defined by 17 STRs (AmpFlSTR YFiler) in Central Portugal

The 17 Y-chromosome STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS456, DYS391, DYS392, DYS393 and DYS385 a/b, DYS458, DYS439, DYS635, GATA H4.1, DYS437, DYS438 and DYS448) were determined for 100 unrelated males, living in Central Portugal, using the AmpFlSTR YFiler PCR Amplification kit (Applied Biosystems).A total of 99 different haplotypes were found, with only two individuals sharing the same haplotype. The overall haplotype diversity (HD) was determined as 0.9998, a value similar to other Y Filer data sets.Y-STR polymorphisms in Central Portugal population, using YFiler, provide a powerful discrimination tool for routine forensic applications.     

Y-chromosome variation in Rio de Janeiro,Brazil, population sample

Allele and haplotype frequencies of seven Y-chromosome STR loci, DYS 19, DYS388, DYS389I, DYS389II, DYS390, DYS391 and DYS392, were determined from 109 unrelated males in Rio de Janeiro, Brazil, sample population.     

Republican liberty and border controls

This article examines the normative status of border controls from a neo-republican perspective, grounded in the value of freedom as non-domination. It makes use of Philip Pettit’s account of this kind of freedom and discusses Pettit’s own remarks on the status of border controls. Against Pettit, it argues that the domination generated by border controls is ineliminable given existing political institutions, because such controls cannot avoid subjecting non-citizens to coercion in ways that are not forced to track their interests. The article also argues for an alternative neo-republican account of border controls that does not deny their coerciveness but allows for certain border control policies in non-ideal circumstances.     

Deep learning for sex determination: Analyzing over 200,000 panoramic radiographs

The objective of this study is to assess the performance of an innovative AI-powered tool for sex determination using panoramic radiographs (PR) and to explore factors affecting the performance of the convolutional neural network (CNN). The study involved 207,946 panoramic dental X-rays and their corresponding reports from 15 clinical centers in São Paulo, Brazil. The PRs were acquired with four different devices, and 58% of the patients were female. Data preprocessing included anonymizing the exams, extracting pertinent information from the reports, such as sex, age, type of dentition, and number of missing teeth, and organizing the data into a PostgreSQL database. Two neural network architectures, a standard CNN and a ResNet, were utilized for sex classification, with both undergoing hyperparameter tuning and cross-validation to ensure optimal performance. The CNN model achieved 95.02% accuracy in sex estimation, with image resolution being a significant influencing factor. The ResNet model attained over 86% accuracy in subjects older than 6 years and over 96% in those over 16 years. The algorithm performed better on female images, and the area under the curve (AUC) exceeded 96% for most age groups, except the youngest. Accuracy values were also assessed for different dentition types (deciduous, mixed, and permanent) and missing teeth. This study demonstrates the effectiveness of an AI-driven tool for sex determination using PR and emphasizes the role of image resolution, age, and sex in determining the algorithm's performance.     

The impact of lipid degradation on fingerprint quality on fired firearm cartridges

The recovery of identifiable fingerprints from fired cartridge cases is challenging. Therefore, the characterization of chemical modifications and their effects on fingerprint integrity post-firing is essential. In this study, the primary fingerprint lipids, including myristic acid, pentadecanoic acid, palmitoleic acid, palmitic acid, stearic acid, squalene, and cholesterol in fired and unfired cartridges, were extracted with acetonitrile, followed by derivatization using N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (BSTFA/1%TMCS). Squalane was used as the internal standard, and all quantifications were performed using gas chromatography coupled with mass spectrometry using a triple-quadrupole mass filter. All lipids identified in the unfired cartridges were also detected in the fired cartridges, and statistical analysis using Student's t-test and F tests was performed with a 95% confidence level. The concentration of lipids in the unfired cartridges was found to be similar to that detected in the fired cartridges, except for squalene, the recovery of which was 28% lower in the fired cartridges.     

Structures,functions and flows of IWT: deconstructing a criminal network between East Africa and Southeast Asia

Crime, Law and Social Change - The paper investigates the role of criminal networks in fostering illegal wildlife trade (IWT), and how these relational structures interact with transnational...