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71.
Szibor R Edelmann J Hering S Plate I Wittig H Roewer L Wiegand P Calì F Romano V Michael M 《Forensic science international》2003,138(1-3):37-43
The incorporation of reference DNA is crucial to the validation of any DNA typing protocol. This paper aims to provide a panel of reference DNAs for actual forensic profiling strategies, i.e. autosomal and gonosomal STR typing as well as mtDNA sequencing. We have characterised three human lymphoid cell lines, GM9947, GM9948 and GM3657, and considered 58 autosomal and gonosomal microsatellites as well as the mitochondrial control region sequence. Well-established markers and STRs recently developed for forensic use were involved. K562 DNA samples which we purchased from two different suppliers were also analysed. They revealed conflicting results with regard to the ChrX STR marker genotype. Hence, we suggest that K562 is no longer used for the calibration of profiling techniques. Our investigation establishes a panel of one female and two male DNA samples as an STR allelic ladder calibration tool and offers information on six alleles of each autosome (AS) marker, three alleles of each X chromosome (ChrX) marker and two alleles of each ChrY marker. In addition, sequences of the mitochondrial control region of the three DNAs are communicated in order to provide sequencing quality control. 相似文献
72.
Reinhard Bendix 《Society》1990,27(3):51-58
His books include Higher Civil Servants in American Society; Work and Authority in Industry; Max Weber: An Intellectual Portrait; National
Building and Citizenship; Embattled Reason: Essays in Social Knowledge; Kings or People: Power and the Mandate to Rule; Force,
Fate, and Freedom;and From Berlin to Berkeley. 相似文献
73.
Verhoff MA Krähahn J Schunk W Heyne M Ramsthaler F Dettmeyer R Kreutz K 《Archiv für Kriminologie》2008,222(1-2):38-51
A human skeleton was found during construction work on the concrete foundations of a fence built 17 years before. The situation seemed to suggest that a corpse had been disposed of 17 years ago. Forensic and osteological examinations showed that death had been caused by semi-sharp and blunt craniocerebral trauma. However, there were morphological findings which indicated that the body had been buried in soil for at least 50 years. Several additional objects found nearby and the proximity to the Limes pointed to a Roman context. With the help of the radiocarbon method, the time of death could be dated to about 245-263 AD. A recent act of violence could hence be ruled out. According to the situation at the scene, it had to be assumed, however, that the skeleton must have been discovered already during the construction work done 17 years before and that the find had not been reported. Such behaviour is a phenomenon quite often encountered in practice. 相似文献
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Reinhard Heinisch 《West European politics》2013,36(3):67-96
While Germany is facing the wholesale disorganisation of sectoral collective bargaining, the Austrian social partnership has gained new strength in the 1990s. Comparatively, Austro‐corporatism proved able to undergo a process of skilful adaptation. This divergence in performance poses a puzzle, given Germany's commanding presence both in international markets and in the European Union, and given Austria's traditional hostility to modernisation. This article explains German—Austrian differences in the performance and resilience of corporatist governance in the face of modernisation and market integration in terms of (i) the organisational differences between German and Austrian corporatism (sectoral concentration versus vertical centralisation and little horizontal formalisation); (ii) the long term policy strategies employed by labour unions in either system (co‐determination versus macro‐level policy influence); and (Hi) by the different responses to modernisation chosen by German and Austrian corporatist actors (internal organisational reforms verus becoming modernisation brokers). 相似文献
78.
Monika H. Seltenhammer Ph.D. Christine Fitzl M.Sc. Ingo Wieser Ph.D. M.Sc. Reinhard Binder B.Sc. Pia Paula M.D. Daniele U. Risser M.D. 《Journal of forensic sciences》2014,59(5):1364-1367
Forensic ballistics is the study of bullet trajectory and consists of determining gunshot residue (GSR) to identify bullet holes. Among several highly sensitive methods, atomic absorption spectrophotometry (AAS) is employed to analyze GSR in the laboratory. However, it is sometimes necessary to identify bullet holes immediately at a crime scene. The purpose of this examination was to determine whether the use of the field test Bullet Hole Testing Kit 3 (BTK3) on a suspected bullet hole would influence the outcome of AAS‐analysis: Three commonly encountered firearms (Glock17, Tokarev, and Colt) were fired at skin, wood, and cloth. AAS‐analysis was performed with and without previous BTK3 application. The results clearly indicate that there is no significant interaction on the grounds of BTK3 use (BTK3 vs. no‐BTK3 [kit_nokit] [Pb: p = 0.1309; Sb: p = 0.9111], material*kit_nokit [Pb: p = 0.5960; Sb: p = 0.9930], distance*kit_nokit [Pb: p = 0.4014; Sb: p = 0.9184], and firearm type*kit_nokit [Pb: p = 0.9662; Sb: p = 0.9885]); hence, applying this field kit does not falsify later AAS outcomes. 相似文献
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X-linked microsatellite markers have proven to be powerful tools for parentage testing, mainly in deficiency paternity cases when the disputed child is female. However, only a small number of X-linked short tandem repeats (STRs) have been comprehensively described for forensic applications to date.We present sequence and population genetic data of the DXS7424 STR (GDB-G00-577-633) which is a trinucleotide repeat polymorphism representing 12 alleles of 147-180 bp in length. DXS7424 is located at Xq22 and closely linked to DXS101, corresponding to a genetic localisation of 104.9-121 cM from Xp-tel.PCR fragment length measurements and sequencing were carried out using the automatic gene analyser ABI 310 (Applied Biosystems).The population of 764 unrelated Germans checked for this STR exhibited the following features: polymorphism information content (PIC) = 0.780; heterozygosity (Het) = 0.843; mean exclusion chance (MEC = 0.766. Kinship tests revealed a typical X-linked inheritance. In 300 meioses under investigation, mutations were not found. Significant deviations from the Hardy-Weinberg equilibrium (HWE) were not established.Linkage studies confirmed closely linkage to DXS101. Additional we found linkage disequilibrium between DXS7424 and DXS101. This requires to use the established haplotype frequencies in kinship testing. 相似文献