早产和胎盘早剥的损伤程度鉴定（附1例分析）

本文从1例孕妇的损伤程度鉴定着手,对《人体重伤鉴定标准》的第78奈、第45条、第92务等条款的应用进行研究,结合案情对现行标准应用误区进行分析,提出症结所在,为了保证鉴定结论的质量,对《人体重伤鉴定标准》进行完善和修改势在必行。     

20世纪80年代以来国内外政府部门顾客满意度研究述评

经济发达国家在20世纪80年代就开始进行顾客满意度的研究和实践。瑞典、美国、挪威、欧盟等许多国家和地区根据各自的实践特点和满意度理论,都相继建立了政府部门顾客满意度指数模型。国内的顾客满意度指数评价制度也从无到有,正逐步进入研究试行阶段。由于社会制度、经济发展水平不同及传统文化的影响,国外政府部门顾客满意度测量体系不适合我国国情。构建我国政府部门顾客满意度指数体系的首要任务是建立适合我国国情的满意度指数模型。     

How “institutionalization” can work. Structuring governance for digital transformation in Italy

Public policy scholars have recently focused on the mechanisms accounting for the sustainability of major policy changes. Among the strategies by which policy entrepreneurs may try to avoid future backlash institutionalization is certainly one of the most used. Yet, it can foster ossification and eventually jeopardize policy effectiveness. Such a potential trade-off between institutionalization and long-term effectiveness is particularly intense in policies concerning technological innovation because the necessity to create winning coalitions can undermine the required absorption capacity needed by government to engage the innovation ecosystems. This paper explores such a trade-off with a case study on the Italian policy for public sector's digital transformation. The case is theoretically promising because over three decades institutionalization has always represented the main overall strategy adopted by policymaker, but only the 2016 initiative emerged as a “success.” In this sense, the case study can focus on the mechanisms activated by policy entrepreneurs to trigger and entrench change.     

Measuring horizontal fiscal imbalance: the case of Italian municipalities

In the literature on fiscal federalism, vertical fiscal imbalance has been widely studied, while the theme of horizontal fiscal imbalance and inequality among local governments, due to differences in their fiscal capacities, has been less explored. This article contributes to fill the gap. A new method to compute fiscal capacities based on regression analysis is proposed, which can overcome some of the drawbacks of traditional methods such as the representative tax system. This new approach is then employed to evaluate the fiscal capacities of Italian municipalities over the period 2002–2010. Finally two global measures of the horizontal fiscal imbalance are used to evaluate the equity implication of a major policy change occurred in 2008 in Italian municipal finance.     

生态文明：发展中国特色社会主义的必然选择

生态文明与人类的生产生活有着极大的关联性。工业文明过程极大地影响和破坏了生态环境。中国的工业化进程,也不同程度地影响和破坏了生态环境。生态文明建设必须遵循人类社会发展规律,以中国特色社会主义理论体系为指导,深入贯彻落实科学发展观。     

改革开放与中国在世界贸易体制内的多边外交

改革开放以来,对外贸易在我国经济和社会发展中的地位日益上升,全方位参与多边经贸外交成为我国面临的一项重大课题。本文通过分析中国在关贸总协定和世界贸易组织所代表的世界贸易体制内的参与及发挥的影响力,对如何进一步发挥中国在该领域的建设性作用进行了展望。     

乙型脑炎病毒E蛋白抗原表位E39特异性单克隆抗体的制备

为了制备乙型脑炎病毒E蛋白抗原表位特异性单克隆抗体,将编码乙型脑炎病毒E蛋白抗原表位E39的DNA序列进行人工合成,随后插入表达载体pGEX-6p-1的限制性酶切位点BarnH I与Xho I之间,构建了表位肽与GST的融合表达质粒.该表位融合蛋白经亲和层析纯化后免疫BALB/c小鼠,按常规方法进行细胞融合.以化学合成的E39表位多肽为抗原,对融合细胞上清进行间接ELISA筛选.结果,筛选出1株分泌E39表位特异性抗体的杂交瘤细胞株,该杂交瘤细胞分泌抗体能力稳定.经鉴定,该单克隆抗体亚型为IgG2b,轻链类型为k链.结果表明,用表位融合蛋白为抗原可以制备表位特异性单克隆抗体.     

Delayed elimination in humans after ingestion of colchicine: Two fatal cases of colchicine poisoning

Colchicine has been widely used in the treatment of acute gout over the years, but it has a narrow therapeutic index, and overdose can be life threatening. A method using ultra-high-performance liquid chromatography–tandem mass spectrometry system was applied in two fatal cases of colchicine poisoning in this study to the determination of colchicine in blood. In case 1, a 19-year-old man suffered from depression and ingested 160 colchicine tablets (each 0.5 mg). The concentration of colchicine in his blood samples showed a fluctuating trend and kept above the therapeutic steady-state concentration for 5 days. In case 2, a 70-year-old female patient with a history of gout and chronic colchicine intake ingested five times the usual dose of colchicine (5 mg) and died after 12 days of medical care, with 5 ng/mL of colchicine in her blood sample. Our findings suggest that the delayed elimination and accumulation in humans after colchicine overdose could keep the concentration of colchicine maintaining above the therapeutic steady-state concentration for many days before dying, probably along with a fluctuating trend.     

论“共同道德”对“永恒道德”的批判

道德的社会存在根源和相对独立性,决定了"共同道德"在一定的条件下是存在的。道德是时代的产物,不存在"永恒道德"。鼓吹"永恒道德"的人其实质是历史唯心主义者。"共同道德"与"永恒道德"毫无共同之处,其理论基础不同,对群众的指导也不同。认真应对"永恒道德"对中国的负面影响,应当加强马克思主义理论的学习,重视意识形态领域的斗争;坚决抵制西方"永恒道德",主动宣传人类"共同道德";批判继承中国传统道德的丰富资源,借鉴吸收人类道德文明的优秀成果;培育社会主义核心价值观自信,促进中国特色社会主义道德建设;用习近平新时代中国特色社会主义思想,筑牢应对错误思想的防线。     

3D forensic facial approximation: Implementation protocol in a forensic activity

The primary objective of this paper is to report on the successful implementation of forensic facial approximation in a real case in the forensic context. A three‐dimensional (3D) facial approximation protocol of the skull was performed with free software, applying techniques in a virtual environment that have already been consolidated in the literature. The skull was scanned with the photogrammetry technique, the digital replica was imported in the Blender software (Blender Foundation, Amsterdam) and individualized model sketches of the face were traced with the MakeHuman software (MakeHuman Org) according to the anthropological profile of the victim. The face created was imported in Blender, where it was adapted, modeled, and sculpted on the 3D skull and its soft tissue markers, using an American open‐source application of the technique in the digital environment. The face created in a virtual environment was recognized and legal identification procedures were started, resulting in the more agile delivery of the disappeared body to its next of kin. It is therefore concluded that facial approximation may not be a primary method of human identification, but it can be satisfactorily applied in the forensic field as an individual recognition resource. It has great value in narrowing the search, reducing the number of alleged victims, and leading to identification tests, therefore significantly reducing the number of genetic DNA (deoxyribonucleic acid) tests—which are considered costly for the State or Federation—and consequently reducing the waiting time before delivery of the body to its family.