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51.
Moreno LI Tate CM Knott EL McDaniel JE Rogers SS Koons BW Kavlick MF Craig RL Robertson JM 《Journal of forensic sciences》2012,57(4):1051-1058
The potential application of mRNA for the identification of biological fluids using molecular techniques has been a recent development in forensic serology. Constitutively expressed housekeeping genes can assess the amount of mRNA recovered from a sample, establish its suitability for downstream applications, and provide a reference point to corroborate the identity of the fluid. qPCR was utilized to compare the expression levels of housekeeping genes from forensic-like body fluid stains to establish the most appropriate assessment of human mRNA quantity prior to profiling. Although variability was observed between fluids and individuals, results indicated that beta-2 microglobulin exhibited the highest expression for all body fluids examined and across donors. A one-way analysis of variance was performed for housekeeping gene variability between donors (at the α, 0.05, significance level), and the results indicated significant differences for semen, vaginal secretions, and menstrual blood. 相似文献
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Peter Freeman 《Intelligence & National Security》2013,28(2):206-228
The War Office's First World War cryptanalytic bureau MI1(b) has been severely overshadowed by its more glamorous equivalent in the Admiralty, ‘Room 40’. In particular its diplomatic decryption work has gone completely unnoticed; yet this was its main activity, and it contributed more than did Room 40 to their common successor, the Government Code and Cypher School (GC&CS). This article, drawing on the past decade's releases of GC&CS archives, traces the development of MI1(b)'s diplomatic work, disentangles its achievements from those of its better-known naval colleague, describes how the two organizations were merged to become GC&CS, and suggests why MI1(b)'s achievements were so quickly forgotten. 相似文献
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Collins PJ Hennessy LK Leibelt CS Roby RK Reeder DJ Foxall PA 《Journal of forensic sciences》2004,49(6):1265-1277
Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR loci plus the gender-determining locus Amelogenin was developed to provide a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and vWA) as well as two internationally-accepted STRs (D2S1338 and D19S433). By incorporating five-dye fragment analysis technology and non-nucleotide linkers, previously optimized AmpFlSTR kit primer sequences have been maintained. This kit has been developed in accordance with the standards of the forensic community as defined by the DNA Advisory Board. Validation studies were performed to include developmental validation, and the results support the use of the AmpFlSTR Identifiler PCR Amplification Kit for human identity and parentage testing. 相似文献
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Innovation research has been characterized by findings which are either considered unstable or inconsistent. This article addresses some of the methodological issues that arise when one attempts to test interactive models of innovation, describes how one particular algorithm — AID-3 — can be used to refine the use of general linear models, and proposes a research strategy based on a building mode of innovation theory. As such it functions as a “how to” for those interested in designing innovation studies and also as a theoretical piece aimed at re-organizing the goals and outputs of innovation research. 相似文献
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