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291.
Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid. 总被引:4,自引:0,他引:4
M N Hochmeister B Budowle O Rudin C Gehrig U Borer M Thali R Dirnhofer 《Journal of forensic sciences》1999,44(5):1057-1060
Prostate specific antigen (PSA, also known as p30), a glycoprotein produced by the prostatic gland and secreted into seminal plasma, is a marker used for demonstrating the presence of seminal fluid. Methods for the detection of PSA include Ouchterlony double diffusion, crossover electrophoresis, rocket immuno-electrophoresis, radial immunodiffusion, and ELISA. The extremely sensitive ELISA technique can detect PSA in concentrations as low as approximately 4 ng/mL. However, all these techniques are cumbersome and time consuming to perform in forensic laboratories, especially when only a few samples per week are processed. Various membrane tests are currently used in clinical settings to screen a patient's serum for the presence of PSA at levels greater than 4 ng/mL. In this study we evaluated three immunochromatographic PSA membrane tests by analyzing semen stains stored at room temperature for up to 30 years, post-coital vaginal swabs taken at different time after intercourse, semen-free vaginal swabs, and various female and male body fluids, including urine. The data demonstrate that PSA membrane test assays offer the same sensitivity as ELISA-based tests and provide a rapid approach for the forensic identification of seminal fluid. Furthermore, when the supernatant from a DNA extraction is used for the assay, there is essentially no DNA consumption for determining the presence of PSA in a forensic sample. 相似文献
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Law and Human Behavior - Relevant to forensic practice, the Supreme Court in Daubert v. Merrell Dow Pharmaceuticals, Inc. (1993) established the boundaries for the admissibility of scientific... 相似文献
293.
Shirley S. Angrist Richard Mickelsen Anthony N. Penna 《Journal of youth and adolescence》1977,6(2):179-186
Studies of college freshmen find that men are more sexist in their attitudes than women. Does this hold also for high school students? This paper reports the relationship of (1) sex-role attitudes to selected school and family characteristics and (2) attitudes toward family life to sex-role conceptions for women and men. The study is based on 529 students in grades 9 to 12 attending four high schools. The findings show that males are more sexist than females and Blacks more so than Whites. Women who are bright, from upper class backgrounds, and whose mothers work are more equalitarian in sex-role conceptions. For men, family background is unimportant, but attending an elite public school seems to foster equalitarian sex-role conceptions. Men's sex-role attitudes are harder to explain than women's, and, unlike women, their role attitudes have little connection to their family orientation. 相似文献
294.
The present study assessed the comparability of adolescents' (N=184), their mothers' (N=184), and their fathers' (N=184) attitudes toward contemporary societal issues, as well as each familial group's perceptions of the other two groups' attitudes. Results of multivariated analyses of variance indicated that while there were significant overall differences between adolescents' and either parent's self-ratings for the 36 questionnaire items (dealing with such topics as drug use, sexuality, and dress codes), major (i.e., 2-scale-point) differences between generational groups existed on only about 20% of the items. However, as predicted, both adolescents and parents misperceived the extensiveness of the divisions between them. Adolescents significantly overestimated the number of major differences between themselves and their mothers and fathers, while these two parental groups significantly underestimated such divisions. These distortions in perceived attitudes were also reflected in the results of correlational analyses assessing intrafamilial attitude consistencies and inconsistencies across the 36 items. Self-alternative-family-members' perceived attitudes correlations showed greater consistency than existed in the self-alternative-family-members' actual attitudes correlations. Moreover, both analyses of variance and correlational analyses indicated that there was greater similarity between the actual attitudes of the mothers and fathers than between either parental group and their children. These results are discussed in terms of the cognitive and emotional significance of the intrafamilial attitudes of adolescents and parents.Received his Ph.D. from the City University of New York in developmental psychology. Current research interests include the relation of organismic variables to personality/social development.Received his Ph.D. in psychology from the University of Denver. Research interests include measurement theory and cognition. 相似文献
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Richard Schultz 《Canadian public administration. Administration publique du Canada》1976,19(2):183-207
In the National Transportation Act of 1967, provision was made, by means of Part III of the legislation, for the federal government to regulate directly the extra-provincial motor carrier industry. This section has never been implemented and this article attempts to explain why. Such an explanation, it is argued, is important because of the philosophy of the NTA, the responsibilities given to the Canadian Transport Commission and the inadequacies of the existing provincial regulatory systems. The explanation is to be found in an analysis of two factors whose interrelationship has been given inadequate attention. The first factor is the growing recognition of the need for intergovernmental collaboration in transportation planning and regulation. The second is the role of regulatory bodies in the policy process. The argument is advanced that the conflict over implementation of Part in illustrates a number of problems with respect to the appropriate functions of regulatory agencies, particularly in the context of intergovernmental negotiations. The article concludes with the suggestion for a possible course of action that may be applicable to other policy areas. Sommaire. La partie III de la Loi nationale sur les transports de 1967 prévoyait la régulation directe par le gouvernement fédéral de l'industrie du transport extre-provincial par véhicule moteur. Ces dispositions n'ont jamais été mises en application et le présent article tente d'en expliquer les raisons. Une explication s'impose, en effet, en raison de la philosophie même de la loi, des responsabilités qui incombent à la Commission canadienne des transports et des faiblesses de la règlementation provinciale actuelle. L'auteur attribue cette situation à deux facteurs dont l'interdépendance a été sous-estimée. Le premier est celui de la reconnaissance accrue de la nécessité d'une collaboration intergouvernementale pour la planification et la régulation des transports. Le second est le rôle des organismes de regulation dans l'élaboration de la politique en la matière. On suggère que le conflit touchant la mise en application de la Partie in de la loi fait ressorti un certain nombre de problèmes relatifs à la détermination des functions propres des organismes de régulation, particulièrement dans le contexte des négociations intergou-vemnementales. L'article propose en conclusion une ligne de conduite qui pourrait convenir à d'autres secteurs. 相似文献
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