Analysis of 2,4-Dinitrophenol in Postmortem Blood and Urine by Gas Chromatography–Mass Spectrometry: Method Development and Validation and Report of Three Fatalities in the United States

2,4-dinitrophenol (2,4-DNP) is a compound used in the early 1900s as a weight-loss drug but later prohibited due to its severe adverse effects, including death. It has however been attracting interest, due to its weight-loss properties, and appears to be re-emerging in forensic casework. As 2,4-DNP is available for use in industry and as a pesticide and easily accessible online, the dissemination of this drug can be fast. The compound exerts its effects through inhibition of ATP synthesis, and corresponding thermogenic energy loss which can be fatal. A method for qualitative and quantitative analysis of 2,4-DNP in blood and urine specimens using GC-MS with hydrogen as carrier gas is described. The method was validated and displayed acceptable performance parameters with linearity (R² higher than 0.998), inter-assay imprecision (lower than 10.6%), intra-assay imprecision (lower than 10.7%), and extraction efficiency (92.1%). Stability of 2,4-DNP in blood and urine was studied, and the drug was stable up to 30 days refrigeration or frozen. Six cases in United States suspected to be related to 2,4-DNP were analyzed. Three cases were found to be positive for 2,4-DNP. Concentrations of 2,4-DNP were in the range of 61.6–220 mg/L in urine and <3–114 mg/L in blood. Based on our findings, we suggest that medical examiners and forensic toxicologists be aware of the reappearance of 2,4-DNP, including this compound as a target in death investigations related to weight-loss drugs.     

Buying Amendments? Lobbyists' Campaign Contributions and Microlegislation in the Creation of the Affordable Care Act

When do campaign contributions matter? This article advances the claim that a group that gives campaign contributions to US Members of Congress is more likely to achieve legislative success when (1) a single legislator can deliver to the group (2) a private benefit (3) without attracting negative attention. Using an original data set based on the written comments of nearly 900 interest groups lobbying the US Senate Finance Committee on health reform legislation in 2009, I link group requests to corresponding legislation. The analysis shows a significant relationship between lobby groups' campaign contributions and their legislative success, and at distinct units of analysis—the group, the side, and the group-senator dyad. The relationship is particularly strong in predicting senators’ amendments in committee. The rare data presented here offer compelling evidence that interest groups' legislative victories are sometimes connected to campaign contributions in a way that previous studies could not identify.     

Are Developmental Assets Protective Against Suicidal Behavior? Differential Associations by Sexual Orientation

Journal of Youth and Adolescence - Sexual orientation disparities in suicide behavior are well-documented. Yet, few studies have examined how developmental assets – key values,...     

Migrant women breadwinners in Italy during the crisis: improvement or trap?

Gender gaps in some aspects of the labour market in Europe narrowed during the recent economic crisis, mainly because men were hit harder and because of the ‘added worker effect’. Therefore, the number of families with a single wage-earner, in particular female-headed households, increased. However, the differential impact of the crisis on male and female labour force in part was an unintended effect of the ‘gendered’ and ‘racialized’ structure of the labour market. Occupational concentration in care and reproductive work and the public sector in fact protected women from unemployment. Adopting an intersectional approach and using individual and household data from the Labour Force Survey from 2008 to 2015, the aim of this paper is to assess to what extent the gendered and ‘racialized’ structure of the Italian labour market has changed, both from a quantitative and qualitative point of view, and to what extent the increase in female breadwinner families, especially among migrants, hides widening or narrowing intersectional inequalities by gender and citizenship.     

Age at Menarche and Adolescent Alcohol Use

Research has shown that early maturation is related to problematic alcohol use, yet the differential effect of early pubertal timing (i.e., younger age at menarche) on the onset of alcohol use and subsequent level of alcohol use has rarely been examined. This distinction is relevant, as younger age at menarche can have differential effects on these outcomes, which in turn can have long-lasting effects. Therefore, the present study examined the relationship between age at menarche and adolescent alcohol use among girls, hereby distinguishing between onset and level of alcohol use. In addition, the moderating effects of alcohol-specific rules, child disclosure and class gender composition were examined. Participants were 430 girls from a Dutch four-wave survey, with a mean age of 12.17 years (SD = 0.50) at the beginning of the study. Results showed that the probability of onset of alcohol use was increased by younger age at menarche, but only when girls were younger than 15. Moderation analyses showed that younger age at menarche increased the risk of alcohol onset only in low risk girls (with high levels of alcohol-specific rules and in classes with a high percentage of girls). Once adolescent girls started drinking alcohol, younger age at menarche was associated positively with alcohol consumption only for girls in classes with a moderate to high percentage of girls. These findings confirm that younger age at menarche is a risk factor for the onset of alcohol use, but strongly suggest that this effect is strongest for girls having restrictive alcohol-specific rules and in classes with a high percentage of girls. Possibly, in the absence of social factors that “push” to alcohol use, biological factors (like age at menarche) become more important. Another possibility is that adolescent girls start drinking alcohol to oppose their parents if they set too strict alcohol-specific rules.     

Maternal and Family Correlates of Intrinsic Religiosity Profiles Among Low-Income Urban African American Adolescents

Journal of Youth and Adolescence - National trends show that African American adolescents, relative to most other demographic groups, are more religious, and show fewer declines in religiosity,...     

Digital Fashion Engagement Through Affect,Personal Investments and Remix

ABSTRACT

As the fashion scene becomes progressively digitalised, new dynamics emerge between consumers, content, and fashion professionals. Reflecting on forms of online interaction, this article explores contemporary audience engagement in the context of mainstream androgynous fashion. First, I consider the effects of the digitalisation of fashion communication in terms of the particular role played by sentiments and emotions. Due to the brevity, immediacy and informality of online communication, I argue, affect emerges as an increasingly crucial component of the fashion discourse. I then turn to an assessment of both the dangerous implications and the positive potential of such phenomenon. On the one hand, I explore cases where members of the public interact directly with fashion intermediaries on social media and highlight how direct engagement leads audiences to internalise the scrutiny of professionals and make emotional investments in their implicit promises. On the other, I examine Tumblr blogs as an example of forms of indirect audience engagement with fashion. In this context, acquiring a distance from fashion mediation, consumers are able to engage in practices of creativity and semantic alterations of the dominant aesthetics.     

Interactions Between Rejection Sensitivity and Supportive Relationships in the Prediction of Adolescents’ Internalizing Difficulties

Rejection sensitivity, the tendency to anxiously or angrily expect rejection, is associated with internalizing difficulties during childhood and adolescence. The primary goal of the present study was to examine whether supportive parent–child relationships and friendships moderate associations that link angry and anxious rejection sensitivity to depression and social anxiety during middle adolescence in an ethnically diverse sample of 277 youth (M age = 14.30 years; 46.93% male). Analyses revealed that angry rejection sensitivity was related to depressive symptoms, but only for adolescents reporting low support from parents and friends. Friend support moderated the association between (1) angry rejection sensitivity and social anxiety, and (2) anxious rejection sensitivity and depressive symptoms. For adolescents reporting low support from friends, support from parents was positively related to social anxiety. Findings highlight the importance of considering relationships in studies of rejection sensitivity and adjustment during adolescence.