Systematic study on STR profiling on blood and saliva traces after visualization of fingerprint marks

This paper describes a systematic study of the influence of optical, physical, and chemical methods used for fingerprint enhancement on subsequent DNA analysis of biological stains. Latent fingerprints as well as fingerprints in contact with blood and saliva on different surfaces were treated with dactyloscopic methods. As a general finding, subsequent STR profiling of the blood/saliva traces led to good results after all the enhancement methods included in this study. Concerning blood enhancement procedures, the airbrush technique showed deleterious effects on subsequent STR analysis in some cases. We therefore recommend the implementation of the layer technique, as it brings advantages for fingerprint enhancement as well. It could also be shown that, as can be necessary in practical casework, two enhancement methods can be performed on a single stain without having influence on STR profiling. In terms of methodological variety, this paper reflects a comprehensive study performed on STR profiling after fingerprint enhancement methods, including rare methods and variations of techniques, which can be a useful alternative in certain case scenarios.     

Developmental validation of a real-time quantitative PCR assay for automated quantification of human DNA

Our laboratory has developed an automated real-time quantitative PCR assay for detecting human DNA. The assay utilizes an in-house, custom-designed TaqMan-MGB sequence-specific probe (CFS-HumRT) and the ABD 7900HT SDS platform. Developmental validation has followed TWGDAM (1) guidelines and demonstrates that the assay is primate specific, is highly sensitive, yields consistent results, and works with human DNA extracted from a variety of body fluid stains. When operating within the dynamic range of the system using high-quality DNA samples. the technique yields similar quantification results to our current QuantiBlot assay with the added benefit of time saving through automation. Furthermore, the QPCR assay identifies how much amplifiable DNA is in a sample and thus has the potential to predict PCR success in downstream applications such as STR analysis.     

Children's acquisition of political opinions

This paper reports and discusses the results of a survey of English and Welsh schoolchildren aged 11–18, conducted in March–May 2003, covering their attitudes to and knowledge of politics, politicians and political issues. It concentrates in particular on the way these attitudes develop as pupils get older, and also discusses the sources of information on which young people depend, and which may be the key to developing this neglected political ‘market’. It finds that pupils are unenthused by politics, but not ‘apathetic’. Both knowledge and interest increase as pupils grow older; however, negative or cynical attitudes to politicians and to political parties seem to grow at the same time. Yet young people also hold positive attitudes to the electoral process in general, though many do not accept that they will have a ‘duty to vote’ when old enough. The media, though distrusted, is the principal source of information, though it is clear that the family is highly influential; schools seem less influential. Copyright © 2004 Henry Stewart Publications