3-Methoxytyramine in the putamen as a gauge of the postmortem interval

The armamentarium of the forensic thanatologist includes the examiner's subjective assessment of bodily changes together with comprehensive evaluation of environmental and associated factors to estimate the postmortem interval (PMI). Of the various objective means, postmortem accumulation of potassium in vitreous humor is a widely used gauge. In view of the considerable variability inherent in these techniques, an additional marker with greater accuracy would be a welcome supplement to these indices. We have developed a method of estimating PMI from the level of 3-methoxytyramine (3-MT) in the dorsal putamen of the brain. The supernatant of a sample of putamen, sonicated in 6 volumes (weight/volume) of 0.1 M perchloric acid and centrifuged at 51 000 times gravity for 10 min, was analyzed for 3-MT using high-pressure liquid chromatography with electrochemical detection. The change of 3-MT in the putamen is linearly related to the PMI (range: 5.5 to 60 h), irrespective of patient age (1 to 84 years). This observation is attributable to several factors, including high substrate (dopamine) concentration, preservation of catechol-O-methyl transferase activity, and an inactivation of monoamine oxidase activity, as a result of a decrease in tissue pO2.     

Three Strikes Legislation and Sentencing Commission Objectives

Between 1993 and 1997, state legislatures across the country passed legislation popularly known as "three strikes and you're out" laws. These laws appeared at a time when sentencing commissions existed in many states, and the commissions were involved in the legislative debates leading to the votes on three strikes laws. Thus, the passage of three strikes laws can shed light on one type of interaction between sentencing commissions and legislatures. This article posits a variety of objectives for sentencing commissions during three strikes debates, each a response to the recurring "pathologies" that appear when legislative, judicial, and executive branch officials create sentencing policy. A survey of states that have passed three strikes laws indicates that sentencing commissions have not made any systematic difference in the legislative debates on these statutes. Commissions have little reason to oppose these laws absolutely, and could lose political credibility by doing so. Commissions have incentives instead to argue for limiting the scope of these statutes. Where commissions have been involved in the debates about habitual felon legislation, they have emphasized limits on judicial discretion, focused on the quality of legislative deliberations rather than on legislative outcomes, and devoted little attention to prosecutorial charging decisions.