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71.
High‐Resolution Melting (HRM) of Hypervariable Mitochondrial DNA Regions for Forensic Science 下载免费PDF全文
Alípio dos Santos Rocha M.Sc. Isis Salviano Soares de Amorim M.Sc. Tatiana de Almeida Simão Ph.D. Adenilson de Souza da Fonseca Ph.D. Rodrigo Grazinoli Garrido Ph.D. Andre Luiz Mencalha Ph.D. 《Journal of forensic sciences》2018,63(2):536-540
Forensic strategies commonly are proceeding by analysis of short tandem repeats (STRs); however, new additional strategies have been proposed for forensic science. Thus, this article standardized the high‐resolution melting (HRM) of DNA for forensic analyzes. For HRM, mitochondrial DNA (mtDNA) from eight individuals were extracted from mucosa swabs by DNAzol reagent, samples were amplified by PCR and submitted to HRM analysis to identify differences in hypervariable (HV) regions I and II. To confirm HRM, all PCR products were DNA sequencing. The data suggest that is possible discriminate DNA from different samples by HRM curves. Also, uncommon dual‐dissociation was identified in a single PCR product, increasing HRM analyzes by evaluation of melting peaks. Thus, HRM is accurate and useful to screening small differences in HVI and HVII regions from mtDNA and increase the efficiency of laboratory routines based on forensic genetics. 相似文献
72.
Pedro Letai 《美中法律评论》2014,(7):793-821
The paper would analyze the law and economics of introducing flexibility in the system of exceptions and limitations on European Copyright Law. Such flexibility would exist in an open norm on the basis of which the courts can decide whether certain uses of copyrighted material are permissible or not, instead of explicitly defining this in the law. First, it would assess problem areas where the lack of flexibility creates legal disputes and potential barriers to innovation and commercialization. Second, it would analyze the economic rationale and economic effects of introducing flexibility. Exceptions and limitations in the current copyright system are meant to balance the protection granted to rights owners with the public interest's need to make certain unauthorized uses. However, this paper would identify a number of situations that do not fit well within the current set of exceptions and limitations and attribute this to a lack of flexibility. Several of these problem areas have given rise to court proceedings with varying outcomes. The interpretation given by courts to existing exceptions and limitations---such as the quotation right, the exception for transient and incidental copying, the private copying exception, and the incidental use exception--is usually too narrow to respond to new technological developments, new developments in the creation process, or new commercialization models. These types of uses generally do not fit the narrowly defined exceptions and limitations and therefore lack legal basis. The same is true for things not yet invented. 相似文献
73.
This article estimates the fiscal impact of coordination failures in intergovernmental fiscal relations. The coordination failures considered here are due to agency problems arising from the delegation of fiscal powers to sub-national governments, and "common pool" problems associated with funding decentralised government spending through intergovernmental transfers. Particular attention is focused on the trade-off between coordination and fiscal decentralisation. Evidence provided for a sample of thirty countries suggests that coordination failures are likely to result in a deficit bias in decentralized policy making, particularly in the case of developing countries, for which the benefits of decentralization may be over-stressed. Developed countries were found to be less adversely affected by coordination failures and have therefore managed to pursue fiscal consolidation in a decentralized setup. 相似文献
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75.
A Morphological and Morphometric Study of Bite Marks Caused by Mice (Mus Musculus) on Different Baits for Forensic Purposes 下载免费PDF全文
Víctor A. Toledo M.V. Gabriel M. Fonseca D.D.S. Ph.D. Paula A. González M.V. Luis Ibarra M.V. Francisco J. Torres T.M. Pedro L. Sáez Ph.D. 《Journal of forensic sciences》2017,62(2):462-467
In animal bites, the dental attributes can be fundamental in identifying the marks made by various species on different matrices. Although rodent bite marks have been studied in the context of postmortem interference, little research has used different baits to analyze these marks linking not only specific behavior patterns but also the possibility of structural damage. Twenty mice (Mus musculus) were exposed to different baits to study their bite marks in a controlled model. The known pattern of parallel and multiple grooves has been seen in all baits, but polyvinyl chloride and fiber‐optic cable were significantly different between each other and the other baits. Some baits showed patterns of anchorage of the upper incisors and space between the lower incisors when gnawing. This technical note represents a novel model of analysis where veterinarians and/or dentists may be asked to give an opinion on alleged animal bite marks. 相似文献
76.
Modelling the relationship between counter‐knowledge and open‐mindedness for policy development 下载免费PDF全文
Pedro‐José Martínez‐Ortiz Sandra Moffett Juan‐Gabriel Cegarra‐Navarro Fernando A. López Hernández 《Journal of Public Affairs (14723891)》2017,17(3)
Counter‐knowledge refers to flaws in citizens' mental models arising from utilization of rumours, inappropriate knowledge structures, outdated routines or procedures. When counter‐knowledge is applied to civil servants, it may result in problems of efficiency, equity and motivation. This paper examines the relationship between counter‐knowledge and open‐mindedness to model a framework for improving city marketing and policy development. This study tested two measurement models. While the theoretical model represents the effect of counter‐knowledge on city marketing and that the latter has a negative effect on open‐mindedness, the alternative model involves that counter‐knowledge has a positive indirect impact on open‐mindedness. These relationships are examined through an empirical investigation of 203 Spanish city halls. The results show a potential positive indirect effect of counter‐knowledge on open‐mindedness through city marketing programmes. This paper clarifies the existing literature, which tries to contribute to the discussion of city marketing, and helps us to obtain meaning out of the relationship between counter‐knowledge, city marketing and open‐mindedness. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
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The aim of the present study was to examine the psychometric properties of the Barratt Impulsiveness Scale version 11 (BIS-11) among a forensic sample of incarcerated male juvenile offenders (N?=?221). Principal Component Analysis with Promax rotation revealed the expected 6-factor structure, but Confirmatory Factor Analysis of the 6-factor first-order and the 3-factor second-order factorial structures did not present sufficiently good fits. Despite that, the Portuguese adaptation of the BIS-11 demonstrated generally acceptable psychometric properties in terms of internal consistency, mean inter-item correlation, convergent validity, discriminant validity, and concurrent validity that justifies its use among the youth forensic population. Statistically significant associations were found with age of crime onset, conduct disorder, crime seriousness, alcohol use and drug use. 相似文献
80.
This paper asks whether citizens judge public administration to be trustworthy using different criteria from other political institutions. Using survey data, we estimate ordered logistic and multivariate regressions to compare the determinants of trust in six different political-administrative institutions. Findings show that social trust, political interest, as well as other individual characteristics, have very similar effects on trust regardless of the institution. The evidence shows that people who are older and more educated, interested in politics, and employed in the public sector, are only slightly more likely to make some sort of distinction. Implications for non-discriminant judgement mechanisms are discussed. 相似文献