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R Roy 《Journal of forensic sciences》1991,36(5):1580-1585
The detection of haptoglobin (Hp) from serum and bloodstains is utilized extensively in forensic science laboratories in order to include or exclude possible donors. There is an increasing need to make the same discriminations utilizing genetic markers from urine samples. This paper describes the use of enzyme immunoassay and Western blotting (electrophoretic) techniques to determine Hp phenotypes from concentrated urine samples. Serum and urine specimens were collected from volunteer donors. The serum sample from each donor was typed for Hp. The urine specimens were concentrated 3000-fold from the starting volume of 15 mL to a final volume of 5 microL and applied to the gradient polyacrylamide gels. This procedure allows the separation of Hp samples into the three common phenotypes as well as the other rare variants found in humans. The Western blotting electrophoretic technique was used to achieve the transfer of Hp bands from the gels to the nitrocellulose membranes. Enzyme immunoassay with goat anti-Hp antiserum and rabbit anti-goat immunoglobulin alkaline phosphatase conjugate were used to identify the Hp bands from the concentrated samples. Specimens stored for six months at -22 degrees C were also concentrated and typed successfully. Recent implementation of drug-screening policies has resulted in an increase in the submission of substituted urine specimens. The above procedure can be used to detect an additional genetic marker from urine samples and thus facilitate the identity of the donor. 相似文献
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Roy Gardner 《Public Choice》1980,35(2):241-252
In this paper, the problem of the Paretian liberal is cast as a preference revelation game whose outcome function satisfies Gibbard's libertarian condition and strong Pareto optimality. Strategic consistency requires that the equilibrium of the game agree with the sincere outcome. It is shown that, whether viewed in a cooperative or non-cooperative context, the liberal social choice function is strategically inconsistent. This result suggests that, from a strategic standpoint, a different resolution of the liberal paradox is desirable. 相似文献
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Peter Cole Adams 《Australian Journal of Public Administration》1981,40(3):219-222
The theme of this conference is "the image of the public administrator". This puts me immediately on the spot, since I suspect that many public servants believe that if that image is less flattering than they would like it to be, the fault lies largely with the press. This notion, which is also popular in the professions, is at once comforting and dangerous. It is comforting because it is always a relief to find that it is someone else's fault that one is unloved. It is dangerous because it encourages a retreat back into complacency. 相似文献
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