Link to live webcast may constitute copyright infringement

Having found that a deep hyperlink on Davis' website to livestreaming of a motorcycle race owned by Live Nation constitutescopying, the court granted Live Nation's Motion for summaryjudgment in its copyright infringement claim and enjoined Davisfrom maintaining the link.     

欧盟“刑事司法区”述评

自建设欧盟"刑事司法区"理念提出以来,欧盟成员国致力于加强彼此之间的刑事合作。在欧盟宪法性条约的导引下,欧盟及其成员国通过制定与实施一系列重点行动计划及项目,在引渡、刑事互助、刑事判决的相互承认等方面取得了突破,一个欧盟范围内的"刑事司法区"正在形成。     

堆型艾美球虫广东株3-1E基因的克隆与序列分析

根据GenBank中登录的堆型艾美球虫31E基因序列,设计了3条引物,以广东株堆型艾美球虫裂殖子总RNA为模板,利用反转录聚合酶链反应(RTPCR)扩增获得了31E基因部分片段,将这一片段克隆至pGEMTEasy载体中,经PCR、限制性内切酶分析和克隆片段的序列测定、比较,证实了克隆片段的可靠性。序列比较发现,所克隆的基因片段与Eimeriaacervulina美国株(US)、E.acervulinaQH株31EcDNA的核苷酸同源性分别为99.0%和99.2%,推导氨基酸的同源性分别为98.2%和97.6%。     

自行车交通事故推断交通方式1例

自行车交通事故中为明确责任大小,往往需要先明确横穿公路者交通方式是骑行还是推行,由于一些客观条件限制,证人证言、现场痕迹等证据不能明确该关键点,这就需要从尸体上寻找相关证据。笔者曾根据现场车辆碰撞痕迹及尸检所见,运用法医学知识,判断死者交通方式1例,现报道如下。案例资料死者吴某,女,31岁。2003年4月8日11时15分,与其丈夫一前一后骑自行车,当到达一划有人行横道线路口时,吴某先横穿道路,被一辆小货车碰撞,经抢救无效死亡。由于证人证言出现矛盾,推行还是骑行无法确定,如骑行,应负次要或同等责任,如推行,则无责任。现场勘查:自…     

High performance liquid chromatographic determination of alcohols with reference to body distribution of methanol

A method has been developed on reverse phase high performance liquid chromatography for simultaneous determination of methyl, ethyl and isopropyl alcohols under refractive index detection using pure water as the mobile phase. A good separation has been achieved between these alcohols. Detector response was linear with a detection limit of 5 mg/100 ml. Recovery studies were performed by adding known amounts of methyl and ethyl alcohols to blood, lung and liver within the range 80–90%. The reproducibility of the results was always >90%. The quantitative distribution of methyl alcohol in postmortem body tissues and fluids has been reported in three cases of poisoning.     

An Australian Empire Patriot and the Great War: Professor Sir Archibald T. Strong (1876–1930)

Archibald T. Strong, born in Melbourne, was the son of an Australian scholar who went to an academic post at Liverpool. The younger Strong received his secondary and tertiary education in England. There, he became proficient in modern European languages and literature. He initially planned a career in the law, but for health reasons returned to Australia to the Department of English at the University of Melbourne. Prior to the First World War, Strong became prominent in Melbourne literary circles and also a prolific commentator on world affairs. As an early member of the Round Table group in Australia, Strong assessed Imperial Germany as posing an existential threat to the British Empire and hence to Australia's security. The nation's future, he believed, lay in unwavering defence of the Empire. Strong evinced a distinct impatience with fellow citizens, especially on the socialist left, who failed, in his view, to understand the realities of Australia's position in the world and what was at stake in the Great War.     

Effect of carcass size on rate of decomposition and arthropod succession patterns.

Decomposition studies were conducted using two carcasses of domestic pigs, Sus scrofa L., 8.4 kg and 15.1 kg in weight, to determine the effects of carcass size on the rate of decomposition, composition of the arthropod fauna, and succession patterns. A total of 46 arthropod taxa were recovered during this study. No size-related differences were observed between carcasses with respect to composition of the arthropod fauna or patterns of succession. A greater number of arthropods were observed attracted to the 15.1-kg carcass, and the rate of decomposition observed was more rapid for the 15.1-kg carcass than for the 8.4-kg carcass. Internal temperatures of the 8.4-kg carcass were more directly related to external ambient temperatures during the fresh and bloated stages of decomposition. During the decay stage, both carcasses generated internal temperatures significantly above ambient temperatures. Following this stage, internal temperatures fell to approximate ambient temperatures during the postdecay and remains stages.     

The forensic medical evaluation of subcapsular splenic injuries

Data on 63 cases of subcapsular spleen lesions are analyzed. Circumstances of trauma infliction period of the 2-nd stage of spleen capsule rupture, morphological types of subcapsular spleen lesions, microscopic changes in different posttraumatic periods are presented.     

边缘无浆体MSP5蛋白基因的克隆及原核表达

参照已发表的边缘无浆体主要表面蛋白5(MSP5)基因的核苷酸序列,设计了1对特异性引物,以边缘无浆体基因组DNA为模板,采用PCR技术扩增获得了MSP5蛋白基因。将其克隆到pGEM-T Easy载体,并进行测序分析。结果表明,克隆的MSP5基因与GenBank上登录的Florida株MSP5蛋白基因的序列同源性达98.6%,编码氨基酸的同源性为99.0%,并且该序列包含有完整的开放阅读框,大小为633 bp。将该基因亚克隆入原核表达载体pGEX-4T-1,构建了重组原核表达载体。将其转化到DH5α宿主菌中,用IPTG进行诱导表达,实现了融合表达,表达产物的分子质量为45 ku。Western-blot分析表明,此表达产物能够被抗边缘无浆体阳性血清所识别。