Optimization of DNA extraction from low-yield and degraded samples using the BioRobot EZ1 and BioRobot M48

Robotic extraction of DNA from dilutions of blood and semen using either the BioRobots EZ1 or BioRobots M48 consistently produced lower recoveries than standard organic extractions of the same samples. In an effort to increase the efficiency of robotically extracted DNA, glycogen and carrier RNA were added following cell lysis. The addition of glycogen, postlysis, resulted in no improvement in DNA recovery with the BioRobot EZ1. However, when carrier RNA was added to the cell lysate of limited and degraded samples extracted on the EZ1 or the M48, DNA recoveries dramatically increased four- to 20-fold. DNA yields obtained by robotic extraction in the presence of carrier RNA were as high, or higher, as those obtained by organic extraction lacking carrier RNA, while experiments that utilized carrier RNA in both types of extractions showed increased sensitivity for both methods. Furthermore, carrier RNA substantially increased the recovery of fragmented DNA with the EZ1.     

A collaborative study of the EDNAP group regarding Y-chromosome binary polymorphism analysis

A collaborative study was carried out by the European DNA Profiling Group (EDNAP) in order to evaluate the performance of Y-chromosome binary polymorphism analysis in different European laboratories. Four blood samples were sent to the laboratories, to be analysed for 11 Y-chromosome single nucleotide polymorphisms (SNPs): SRY-1532, M40, M35, M213, M9, 92R7, M17, P25, M18, M153 and M167. All the labs were also asked to submit a population study including these markers. All participating laboratories reported the same results, indicating the reproducibility and robustness of Y-chromosome SNP typing. A total of 535 samples from six different European populations were also analysed. In Galicia (NW Spain) and Belgium, the most frequent haplogroup was R1b*(xR1b1,R1b3df). Haplogroup F*(xK) is one of the most frequent in Austria and Denmark, while the lowest frequency appear in Belgium. Haplogroup frequencies found in this collaborative study were compared with previously published European Y-chromosome haplogroup data.     

Exporting corporate social responsibility into Africa? The experiences of South African companies in Swaziland

The economic expansion of South African corporates across the continent is an evolving and contested process. This paper seeks to inform knowledge on the experiences of South African companies expanding into Africa by looking at the social investment approach of five large companies within South Africa and in their Swaziland operations. While social responsibility programmes are proving to be useful for the private sector, the experiences of these companies highlight the need for more studies to find evidence on the developmental role of major private sector players like South Africa in the region and the impact of corporate social investment and corporate social responsibility in Africa.     

Linguistic Diversity and Ideologies among the Catholic Minority in Mandate Palestine. Fear of Confusion or a Powerful Tool?

Abstract

Palestinian Catholics have always played a major role in the Palestinian political, cultural and educational systems, with an influence disproportionate to their numbers. Instrumentalized by France during and even after her Protectorate of the Christians (1924), more visible for the Europeans during the growth of European institutions in the Holy Land and the beginning of an international Christian network, Catholic Palestinians (mainly Latin and Melkite) favoured multilingualism, but at the same time felt trapped between different trends that influenced linguistic ideologies and practices. They faced the centralizing Catholic interests of Rome (who first favoured French and Italian, but soon after the Mandate mostly Arabic); the national interests of Catholic European powers present in the Holy Land favouring their own languages (French, German through the German Catholics and the Austrians, and English through the British Catholics); and the Arabization promoted by the Melkite community.

The present article aims to analyse the linguistic choices of the Catholic community, via its educational system, by observing the process through which a complex local reality has been simplified by colonial powers, to tackle identity and conflict through language.