The effect of sodium fluoride preservative and storage temperature on the stability of cocaine in horse blood, sheep vitreous and deer muscle

The in vitro stability of cocaine in horse blood, sheep vitreous humour (VH) and homogenised deer muscle is described. The stability of cocaine in horse blood was of interest because many toxicology laboratories utilise horse blood for the preparation of calibration and check standards and the latter are typically stored during routine use. The storage stability of cocaine in human VH and muscle has not been previously reported. In the absence of blank human VH and muscle, cocaine stability under varying conditions was demonstrated in animal tissues. Blood and VH were stored with and without addition of NaF at room temperature (RT), 4°C and -18°C for 84 days. Muscle homogenates were prepared in water, water/2% NaF, and phosphate buffer (pH 6.0)/2% NaF, and stored for 31 days at RT, 4°C and -18°C. Cocaine stability in human muscle obtained from cocaine positive forensic cases was assessed following storage at -18°C for 13 months. Cocaine and benzoylecgonine (BZE) were extracted using SPE and quantified by GC-MS/MS. Cocaine was stable for 7 days in refrigerated (4°C) horse blood fortified with 1 and 2% NaF. In the absence of NaF, cocaine was not detectable by day 7 in blood stored at RT and 4°C and had declined by 81% following storage at -18°C. At 4°C the rate of cocaine degradation in blood preserved with 2% NaF was significantly slower than with 1% NaF. The stability of cocaine in horse blood appeared to be less than that reported for human blood, probably attributable to the presence of carboxylesterase in horse plasma. Cocaine stored in VH at -18°C was essentially stable for the study period whereas at 4°C concentrations decreased by >50% in preserved and unpreserved VH stored for longer than 14 days. Fluoride did not significantly affect cocaine stability in VH. The stability of cocaine in muscle tissue homogenates significantly exceeded that in blood and VH at every temperature. In preserved and unpreserved samples stored at 4°C and below, cocaine loss did not exceed 2%. The increased stability of cocaine in muscle was attributed to the low initial pH of post-mortem muscle. In tissue from one human case stored for 13 months at -18°C the muscle cocaine concentration declined by only 15% (range: 5-22%). These findings promote the use of human muscle as a toxicological specimen in which cocaine may be detected for longer compared with blood or VH.     

Assessing trait memory distrust: Psychometric properties of the Squire Subjective Memory Questionnaire

Purpose. When people suffer from memory distrust, they evaluate their memory in negative terms. Memory distrust plays an important role in police interrogations because it may underlie false confessions. The Squire Subjective Memory Questionnaire (SSMQ) intends to be a simple measure of trait memory distrust. To our knowledge, no data have been published about the psychometric properties of the SSMQ. Methods. Relying on five samples (Ns = 70–819) of healthy individuals and patients, we studied the psychometric characteristics of the SSMQ. Participants were recruited at the university, a neuropsychological unit or through advertisements in newspapers. They were asked to complete the SSMQ and several other tests. In this way, reliability and validity parameters could be assessed. Results. Principle component analysis showed that the SSMQ has a one‐dimensional structure referring to subjective memory evaluation. The SSMQ was found to have adequate reliability and good construct validity. Furthermore, it appears to correlate in a theoretically meaningful way with age and cognitive failures. Conclusions. All together, the SSMQ is a psychometrically sound screening tool that can be helpful in assessing subjective memory evaluations in the legal domain. In this way, vulnerable suspects can be identified in an early stage.     

Ethnicizing citizenship,questioning membership. Explaining the decreasing family migration rights of citizens in Europe

Citizenship does not equal belonging. In this paper, we investigate how the disjunction between the ‘imagined community’ and the formal citizenry impacts on citizens’ rights. In particular, we analyse decision-making on the family migration rights of citizens in France, Germany and the Netherlands. Our analysis shows that in these three countries, notwithstanding their different migration and citizenship regimes, the reduction of citizens’ family migration rights is based on the same discursive mechanism: the ‘membership’ of citizens of migrant origin who marry a partner from abroad is called into question. As they are excluded from membership of the imagined community, their entitlement to family migration rights is decreased. Ethnic conceptions of national community, intersecting with gender and class, play a crucial role in shaping the rights attached to citizenship in Europe today.     

Doing family: Responses to the constructions of ‘the migrant family’ across Europe

This paper draws on how constructions of ‘the migrant family’ in political discourse influence migrants' and their families' lives. In specific national contexts, ‘the migrant family’ is determined according to the national and European debates and expressed by their respective rules and regulations. By ‘doing family’, migrants and their families develop strategies in order to fit these requirements of living a certain family life. Fulfilling specific norms and perceptions which are not necessarily required for the majority of society is a precondition to succeed. Who is and who is not part of the family, who holds responsibility — such aspects have to be proved and repeatedly reproduced by migrants and their families. This not only affects their position in society, but also has strong implications on their lives as a couple and family, since it requires the continuous adaptation and reconstructions of their everyday reality.     

Outline Shape Analysis on the Trochlear Constriction and Olecranon Fossa of the Humerus: Insights for Sex Estimation and a New Computational Tool,

Sex estimation through visual analysis of the distal humerus can contribute to establishing the biological profile of an unidentified skeletal individual. Using statistical shape modeling, the trochlear constriction open curves and olecranon fossa closed outlines of 151 humeri were digitized and analyzed. The shape configurations exhibited strikingly different degrees of sexual dimorphism when evaluated using linear discriminant analysis with leave‐one‐out cross‐validation. The trochlear constriction performed poorly, correctly classifying 63.6% of the individuals. However, the olecranon fossa showed high sexual dimorphism, presenting a 94.0% accuracy. A simpler model using only two principal components was also generated. While the accuracy is slightly inferior (88.1%), it has the advantage of being constrained to bidimensional components that were translated into morphoscopic variables within a simulator interface. This allowed us to implement the method through a web application that does not require users to be trained in landmark digitization or have knowledge of geometric morphometrics.     

Unsettling Master Categories: Notes on Studying the Global in C. W. Mills’ Footsteps

C. W. Mills’ sociology arises out of the problematizing of established “truths.” The aim becomes a sociology that unsettles—not a sociology of unsettlement but a sociology that is itself unsettling. In this short essay I focus on themes that capture one way (mine) of executing C. W. Mills’ challenge to us, academic sociologists. The substantive focus is on what is needed to develop critical globalization studies. The accepted narratives and explanations of globalization have produced the global as a master category that obscures as much as it reveals. We need to generate new questions for research to recover what has been excluded by dominant narratives. And we need to develop conceptual architectures that allow us to detect what we might think of as countergeographies of globalization. Here I focus particularly on types of spaces where we can find resistance to global power and as yet unrecognized forms of participation by actors typically represented as powerless, or victims, or as uninvolved with global conditions. The overall project is a critical remapping of the analytic and political terrain of the global.     

Atypical injury pattern of a pedestrian run over by an unimog in the epigastric region

Strongly intoxicated, a 37-year-old man fell in front of the right back wheel of an emergency vehicle (MB Unimog) and was run over according to eye witnesses. He died in hospital shortly afterwards. The autopsy revealed that he bled to death from a traumatic liver rupture (bursting of the right hepatic lobe and severing of a piece of tissue measuring 17 x 8 x 4 cm). There were no injuries classicaly seen in victims run over by a car. The atypical injury findings in this case are due to the special features of the accident vehicle: The Unimog (an all-wheel vehicle with a fixed rear axle and flat coils) struck the right side of the body lying on the street with its rear wheel and was then lifted over the body by its fixed axle without touching the left side.     

Prevalence of Intimate Partner Violence in Thailand

There is no recent national data on the prevalence of intimate partner violence in Thailand. This study proposed to examine the prevalence of intimate partner violence in 4 regions of Thailand by using a standardized questionnaire from the WHO multi country study on women’s health and domestic violence. Two thousand four hundred and sixty-two married or cohabiting women aged 20–59 years were interviewed about their experiences of psychologically, physically, sexually violent, and/or controlling behaviors by their male partners. The study found that 15% of respondents had experienced psychological, physical, and/or sexual violence in their life time which suggests that 1 in 6 of Thai women have faced intimate partner violence. Of the 15% of women who reported intimate partner violence within the past 12 months, psychological violence was the most common (60–68%), followed by sexual violence (62–63%) and physical violence (52–65%). In addition, the percentage of women who faced various forms of controlling behaviors varied from 4.6% to 29.3%. Men who were more controlling were more likely to abuse their female partners. The results reveal that partner violence against women is a significant public health issue in Thai society that must be addressed.