Brain amino acid abnormalities in liver disease--a postmortem study

In a postmortem exploratory study, we examined whether specific amino acid abnormalities associated with liver diseases in vivo may also be detected in human brain samples obtained at clinical autopsies. The branched-chain amino acids (BCAA: valine, leucine, isoleucine) were decreased in the group of patients with liver diseases compared with the control group, whereas the aromatic amino acids (AAA: phenylalanine, tyrosine) were increased. However, the ranges overlapped significantly and were not statistically different. The molar ratio BCAA/AAA was determined to be 1.92 in the collection of patients with liver diseases compared with 2.27 in the control group. In patients with liver disease, ornithine concentrations in the brain appeared significantly decreased whereas glutamine was significantly increased. No significant difference was found in the brain concentrations of proline. Amino acid analysis may contribute to the understanding of pathophysiological mechanisms of liver disease, which are discussed, and may supplement the postmortem diagnosis.     

A hopeless case?

In a hollow covered with earth, a herb gatherer discovered a severely decomposed corpse mutilated by scavenging animals. Only small areas of the skin had remained intact. A conspicuous tattoo could still be distinguished on the left upper arm. Despite concrete evidence of violence (fracture in the occipital bone, subdural hemorrhage with a volume of 20 ml), the exact cause of death could no longer be established. Police enquiries identified a person in whom such a conspicuous tattoo had been photographed in an earlier criminal case. Eventually, the deceased person could be identified by DNA analysis of a stored blood alcohol specimen. Considered together, the autopsy findings and the results of the investigation of traces of blood on the putative tool used in the crime indicated that the man had been struck dead and the body had afterwards been buried in the wood. Although the prospects of success had been initially slight, it was not only possible to identify the victim, but also to reconstruct the crime.     

Molecular characterization and Austrian Caucasian population data of the multi-copy Y-chromosomal STR DYS464

DYS464 is a multi-copy STR system with four positions on the Y-chromosome (DYS464a, b, c, and d) which was recently identified and characterized [Forensic Sci. Int. 130 (2002) 97]. The aims of our study were to perform a population study, to estimate the mutation rate and an extensive sequence analysis in order to confirm the nomenclature. Fourteen different alleles were found in an Austrian population sample with an allele length varying from 9 to 19 repeats. All alleles were cloned and sequenced. Alleles 9-19 showed the general repeat structure (CCTT)n...(CCTT)2...(CCTT)3...(CCTT)4...(CCTT)2...(CCTT)2. The nomenclature is based on the number of repeated units of the variable (CCTT)n-stretch only. In 13% of the samples intermediate alleles, namely 14.3A, 14.3B and 15.3 were detected. In these alleles the variable repeat block is interrupted by a CTT motif (14.3A: (CCTT)3CTT(CCTT)11; 14.3B and 15.3: (CCTT)7CTT(CCTT)7/8). A comparison with GenBank entries revealed the existence of a length variant due to a deletion of one cytosine in the 5' flanking region of the first repeat block. We designed an alternative forward primer to circumvent possible ambiguities in the allele designation. A total of 54 different genotypes were identified in 135 men corresponding to a discrimination capacity (DC) of 40% and a gene diversity (GD) of 0.97. These values are much higher than those of other Y-chromosomal short tandem repeats (Y-STRs). DYS464 has the same haplotype diversity (HD) as the combination of the five Y-STR loci with the lowest gene diversities of the Y-STR core set. On the other hand, a combination of the three most diverse loci (DYS464, DYS385 and DYS390) has the same capacity to distinguish between paternal lineages than the complete minimal haplotype (minHT) consisting of eight Y-STR loci. In our population sample the addition of DYS464 to the minHT increases the number of different haplotypes from 110 to 122. The mutation-rate estimate based on the 70 meioses analyzed amounts to 2.86 x 10(-2) (95% confidence interval 3.5 x 10(-3) to 9.95 x 10(-2)). This value is approximately 10 times higher than the average mutation-rate estimate for Y-STRs.     

Duplications of the Y-chromosome specific loci P25 and 92R7 and forensic implications

In the present study, we demonstrate that two commonly used Y-chromosome single nucleotide polymorphisms (SNPs), P25 and 92R7, are paralogous sequence variants (PSVs) originating from segmental duplications and that at least one of the sequence variants in each group of loci is polymorphic. Several methodologies were used in order to detect the SNP alleles and the PSVs of the loci. All results obtained with the various typing techniques supported the conclusion. The allele distributions of the binary markers were analysed in more than 600 males with seven different haplogroups. For P25, the ancestral allele C was found in several samples from different haplogroups. The derived allele A was always present with an additional C variant. Haplogroup P was defined by the derived allele A at the 92R7 locus. However, the ancestral allele G was always associated with an A variant due to the duplication.     

Together again: reuniting political theory and international relations theory

This state of the discipline article discusses a body of recent literature that seeks to reunite political theory and international relations theory. It briefly explores some of the factors and explanations that led to a divorce between the sub-fields of PT and IR. The article proceeds to review work that seeks to bridge the dichotomy that came to define the relationship between these two academic fields of study. By examining literature in the area of normative theory, democratic theory and that falling under the rubric of identity and difference, the article attempts to demonstrate that an effort is under way to reunite political theory and international relations theory.     

Warum Mittelmaß? Deutschlands Bildungsausgaben im internationalen Vergleich

Ohne Zusammenfassung Der vorliegende Beitrag ist die überarbeitete und erweiterte Fassung der Antrittsvorlesung des Verfassers an der Ruprecht-Karls-Universit?t Heidelberg am 13. Dezember 2001.     

The Politics of Justice and the Paradox of Justification

Pluralism, (normative) uncertainty, and disagreement are all widely held to constitute major obstacles for gaining assent to policy choices, especially when they concern legitimation-sensitive issues and hence are in particular need of a sound justification. Plausible as it appears to be, in the present article I argue that this belief may reflect only half of the truth, because the very forces that are held responsible for the erosion of consent often also seem to serve as justificatory resources, opening the door for a wide range of policy options that it would not be possible to sell if it were always unambiguously clear what ought to be done. Some empirical examples are discussed to illustrate how this paradoxical logic of justification works in practice.     

Political interest furthers partisanship in England,Scotland, and Wales

According to much of the literature, partisanship in Britain exercises little independent influence on the vote but merely reflects voters’ prospective and retrospective evaluations of the parties’ performance with regard to their management of the economy, national security, and public services. In this view, partisanship comes close to Fiorina’s model of a “running tally” of political experiences. Similarly, Dalton’s notion of “cognitive mobilization” suggests that seeking out political information should undermine both the need for and the likelihood of party identification. Applying Mixed Markov Latent Class Analysis to the British Election Study Panel 1997–2000, we challenge these perceptions by demonstrating that partisanship is more stable than previously thought, and that high levels of political interest are linked to higher levels of partisanship and possible also to higher levels of stability. This is much more in line with classic ideas about party identification than with “revisionist” critiques of the Michigan model, and with current models of political cognition. Moreover, it suggests that political interest renders affective ties more powerful in stabilizing themselves.     

Popliteal Vein Blood Sampling and the Postmortem Redistribution of Diazepam,Methadone, and Morphine

Postmortem redistribution (PMR) refers to the site‐ and time‐related blood drug concentration variations after death. We compared central blood (cardiac and subclavian) with peripheral blood (femoral and popliteal) concentrations of diazepam, methadone, and morphine. To our knowledge, popliteal blood has never been compared with other sites. Intracardiac blood (ICB), subclavian blood (SB), femoral blood (FB), and popliteal blood (PB) were sampled in 30 cases. To assess PMR, mean concentrations and ratios were compared. Influence of postmortem interval on mean ratios was also assessed. Results show that popliteal mean concentrations were lower than those for other sites for all three drugs, even lower than femoral blood; mean ratios suggested that the popliteal site was less subject to PMR, and estimated postmortem interval did not influence ratios except for diazepam and methadone FB/PB. In conclusion, our study is the first to explore the popliteal site and suggests that popliteal blood is less prone to postmortem redistribution.     

The effect of choice reversals on blindness for identification decisions

The present study examined blindness for identification decisions from target-present (TP) and target-absent (TA) lineups using a field study methodology. Eighty pedestrians were exposed to a staged theft. Subsequently, they were asked to identify the thief and the victim from separate, simultaneous six-person lineups. Their identification decision concerning the thief lineup was manipulated such that participants’ selections were exchanged with a previously unidentified lineup member (choice exchange) and lineup rejections were turned into identifications (choice reversal). Participants were 7–10 times less likely to detect choice exchanges (66.7%) compared with choice reversals (11.2%). Furthermore, identification accuracy was not a prerequisite for detection. Thus, rejections and particularly selections made from both TP and TA lineups are susceptible to choice blindness. Finally, our study implies that for blindness in eyewitness identification decisions between-category changes (i.e. choice reversals) are easier to detect than within-category changes (i.e. choice exchanges).