The hoe and the vote: Rural labourers and the national election in Brazil in 1974∗

In this article notions about the political attitudes of rural labourers in present‐day Brazil—such as ‘labourers have no opinion. They vote for whom they are told‘—are questioned. It is shown that among a group of labourers in the interior of the state of São Paulo there is a well‐developed sense of class identity and of the need for solidarity (although actual solidarity is very limited'). Their lack of involvement in national politics in contrast with their activity in local‐level politics (an activity which is, however, non‐party and personalised) and their apparently contradictory views of the nature of the Brazilian state are seen to be perfectly rational responses to the objective circumstances in which they live and work. Moreover, they have well‐developed political attitudes. The article demonstrates in what way the life‐experience of the group and the resulting world view are the basic elements which help to explain the labourers’ ideas of the political struggle and their approach to the electoral process; and it shows that the structurally different roles of men and women are reflected in their respective political attitudes.     

Human being eaten by his own dogs: Genetic confirmation through analysis of bones recovered in a dog's stomach content

A part of a decomposed human body from an individual was found at his home, together with the decomposed bodies of his 3 dogs. The disappearance of half of the human body was hardly explained. Hypothetically the dogs, starving, could have eaten their owner after his death. All the bodies were recovered for autopsy.Small bone fragments were recovered in one dog's stomach and identified as human by anthropological analysis. DNA was extracted and cytochrome b gene analysis was made in order to determine their origin, confirmed as human.Genetic identification allowed achieving an eight mini-STR profile with MiniFiler (Applied Biosystems) identical to the bone material collected at the victim's autopsy, confirmed that the dogs had effectively eaten their owner.The results showed that it is possible to obtain nuclear DNA in samples subjected to gastric acids and the combination of different techniques allowed us to determine, in each step, the most convenient workflow for the remains identification.     

Mini-STRs: A powerful tool to identify genetic profiles in samples with small amounts of DNA

Degraded human remains and crime scene evidences with small amounts of DNA typically reveal incomplete or null genetic profiles when using standard (large) STR amplicons. The technology of mini-STRs, using reduced-size STR amplicons, can help to recover information from these samples. In our Forensic Genetic Service several genetic profiles were obtained or completed using MiniFiler kit (Applied Biosystems) increasing the success rate in sample typing. In all studied cases no inconsistencies were found between profiles obtained with MiniFiler and Identifiler, suggesting that this mini-STR kit can be used to include low copy number (LCN) evidence profiles in STR databases.     

The application of pancuronium bromide (Pavulon) forensic analyses to tissue samples from an "Angel of Death" investigation

The case report of a serial killer who worked at several hospitals as a respiratory therapist is presented. The suspect was initially labeled a benevolent Angel of Death who ended the suffering of elderly patients through mercy killing. However, his subsequently declared motive for homicide was very different from other similar cases in medical settings. The application of new analysis techniques for the detection of pancuronium bromide in a series of aged exhumation tissues gave positive results and led to the resultant conviction of the therapist.     

Voltammetric analysis of luminescent markers in gunshot residues

Currently, SEM-EDS is used to detect gunshot residue (GSR) from the presence of Ba, Pb, and Sb in the sample. However, the development of new nontoxic ammunition (NTA) has prevented conventional metals from being found. In this work, we aim to determine the presence of an inorganic luminescent chemical marker based on rare earth in gunshot residues using the technique of squarewave voltammetry (SWV). After firing, the luminescent complex [(Eu₂Zr)(btc)₃(Hbtc)_0.5.6H₂O], which is used as a chemical marker, can be detected under a UV lamp. An aqueous solution with 0.1 mol L⁻¹ KCl as supporting electrolyte can be easily collected on carbon paste electrode surfaces for SWV analysis A = 100 mV, f = 10 Hz, and step potential of 5 mV are required. The luminescent marker incorporated into the carbon paste electrode showed two anodic peak currents in the region of 0.4 V (vs Ag/AgCl) and at 0.75 V (vs Ag/AgCl) and also a cathodic one in 0.4 V (vs Ag/AgCl). SEM-EDS was able to analyze the same voltammetric results for conventional and nontoxic ammunition containing the luminescent marker. Therefore, voltammetry and SEM-EDS are valid for detecting the new residue marker in GSR. Despite this, the electrochemical method is still more advantageous because of its low cost and lack of expensive equipment and supplies in forensic laboratories.