一种改良的精斑涂片DNA检验方法

目的建立一种有效的精斑涂片DNA检验方法。方法以本实验室平时积累的91例精斑涂片为研究对象,采用chelex法提取,结合硅珠法纯化浓缩,提高DNA浓度后进行PCR扩增和STR分型。结果尽管涂片上精子量较少,但chelex法的DNA提取率较高,且硅珠法可以纯化并浓缩模板DNA,本文建立的方法兼具了这两种提取方法的优点,成功率较高。结论精斑涂片可以作DNA检验,在法医检案中具有较高的应用价值。     

我国构建低成本政府的内在困境分析

政府成本在中国的出现是在致力于建立完善的社会主义市场经济体制中提出的。低成本政府的构建无疑会推进社会主义市场经济发展,成为国家政治、经济体制创新的又一着力点。对于构建低成本政府,从一开始学者们就提出了多种方法。但是对现实具体环境缺乏有效的现实指导力。要顺利地构建有效的低成本政府,必须尽快地从根本上解决其内在的三重困境:理念困境、动力困境和路径困境,从根本性和长远性出发,使构建低成本政府的努力成为一种可能的制度变迁选择。     

农民工权益保障缺失:现状、根源、对策--以构建和谐社会为视角的解读

农民工权益保障缺失是当前构建和谐社会的主要矛盾之一,它已成为考验我们党执政能力的一个严峻课题。二元户籍制度、歧视性就业制度、二元社会保障制度、公众利益诉求机制不完善等体制造成的不平等国民待遇是农民工权益缺失的制度根源。为破解这一难题,一方面应当在宏观的制度层面上恢复农民工的国民待遇;另一方面应采取诸如完善立法、加强执法、规范劳动关系、搞好培训及创新工会组织形式、发挥工会维权作用等针对性举措来维护农民工权益,构建和谐社会。     

加强高校学生党组织工作的思考

培养什么人、如何培养人,是我国社会主义教育事业发展中必须解决好的根本问题。大学生是国家宝贵的人才资源。要使大学生真正成为国家建设的主力军、生力军,不仅要大力提高他们的科学文化素质,更重要的是提高他们的思想政治素质。而强化学生党组织建设和党组织活动,是高校思想政治工作中必不可少的重要环节。1.配合高校“两课”教学,积极发挥学生党组织的作用。政治理论课和思想品德课是对当代大学生进行马克思主义理论教育和思想品德教育的主渠道、主阵地。高校在坚持“育人为本、德育为先”的教育目的下,积极发挥学生党组织的作用,是真正…     

政府职能社会化的法制保障

政府职能的社会化是转变政府职能,解决官僚行政内在不足的根本出路。这一改革需要有宏观的法律制度,也必将引起政府行为方式的变化,而监督与救济机制的完善是各方主体法律权益得以实现的保障。     

科尔曼理性选择理论评述

理性选择理论是社会学的重要理论之一。科尔曼是公认的这一理论的权威人物。科尔曼在“理性人”假设和个人主义方法论的基础上, 认为个体行动者总是依据最大限度地获取效益的原则在不同的行动或事物之间进行有目的的选择。科尔曼理性选择理论将宏观和微观分析相结合, 提出了社会理论研究与发展的新方向。     

论公共服务的社区化——以昆明市盘龙区劝学社区为例

公共服务社区化已成为世界性的选择。社区成为全球政府实现治道变革的平台,它能提高公共服务的供给效率,有利于形成政府与公民“双赢”的局面。目前我国公共服务社区化中存在的问题是:“行政化倾向、市场化倾向、搭便车和不规范行为等。公共服务社区供给的改革应包括:对社区供给的繁复的公共服务进行科学定位、加强社区自治组织架构的建设、与政府合作、建立社区公共财政体制、探索多中心集体选择的激励机制和提升社区能力。     

New Migrants and the Revival of Overseas Chinese Nationalism

Emerging at the turn of the twentieth century, overseas Chinese nationalism played an important part in the evolution of the overseas Chinese community and modern history of China. It is generally held that overseas Chinese nationalism had died out and had become ‘a historical phenomenon’ by the 1950s, when the China-centered allegiance of the overseas Chinese was replaced by a local-oriented identity. The fundamental change of the Chinese diasporic communities over the last two decades, however, has put this conventional wisdom into contestation. This essay is concerned with the emergence of the xin yinmin (new migrants) and corresponding manifestations of a reviving overseas Chinese nationalism since 1980. It is divided into two main parts. The first is empirical, examining the rise and characteristics of the new migrants, who are composed of four main types: students-turned-migrants, emigrating professionals, chain migrants, and illegal immigrants. The second section is conceptual and comparative, looking at the manifestations and limitations of the reviving overseas Chinese nationalism and placing them in a historical perspective. It also considers the embedded tensions between nationalism and transnationalism and the strategies employed by both the Chinese state and new migrants in tackling these tensions. I argue that there are complex reasons behind the re-emergence of overseas Chinese nationalism. From overseas, it is a by-product of the formation of sizeable new migrant communities, particularly in the West. As the first-generation immigrants who have extensive links with the homeland, they remain culturally, and often, politically, attached to China (as a nation-state and/or site of transnational imaginary) and are concerned greatly with Chinese matters. Through such intermediaries as Chinese-language newspapers, websites and TV programs, they form a borderless and imagined greater China that is bound by both the ideas of sovereignty and transnational culture. From the mainland, a series of policies relating to the Chinese overseas facilitate the connections between China and its population overseas, thus providing a potential ground for the revival of overseas Chinese nationalism. I argue that the key agendas of this reviving overseas Chinese nationalism are China's economic prosperity, cultural regeneration, and national unification. This nationalism, furthermore, is characterized by its reactive nature and embedded contradictions with the simultaneous process of transnationalism, which in turn reduces the centrality and intensity of nationalism. As a result, it is unlikely to constitute a unified ideology or a movement with centralized leadership such as that in the 1930s.     

女性与体育：社会性别视角下的审视

在对体育的参与和认知上,女性与男性有很大的区别,这种区别是历史、文化、大众传媒和教育多种因素影响的结果.只有制订相关的社会政策,营造有利的运动环境,才能促进女性对体育运动的积极参与.     

大学生职业生涯规划中的四大理念

不同的职业往往决定着不同的人生。善于规划自己的生活,规范自己的职业行为,设计自己的职业生涯的人更容易获得成功。俗话说得好:“思路决定出路”。但是当前许多青年,特别是大学生在职业生涯规划中出现一系列问题,陷入误区,导致职业生涯受挫,这涉及到一个职业生涯规划的理念问题。大学生在做职业生涯规划时,必须贯穿以下四大理念。小袁是某大学行政管理专业的一名学生,在大一的时候认识一位同系同专业的学姐小丽,两人有相同的兴趣爱好,所以很合得来。后来,学姐毕业后顺利地进入了一家大型企业工作,她非常羡慕,觉得小丽的职业生涯就是她的样…