组织细胞DNA含量变化与死亡时间的相关性研究

目的研究人死后各器官组织细胞DNA降解变化规律,探讨其在推断死亡时间方面的应用价值,为推断死亡时间提供科学依据。方法已知死亡时间人尸体的心、肝、脾、肾,按死后6,12,24和48h四个时间段取材,制成石蜡切片,经Feulgen染色并图像分析;同时将各器官组织按不同时间段取材,制成单细胞悬液,应用流式细胞仪检测DNA含量。结果(1)人体各器官组织细胞经FCM检测,脾细胞核DNA随死亡时间延长呈现较好的下降梯度,而心、肝、肾,则由于自溶较快,死后6h细胞核DNA含量已很低,其变化情况与死亡时间的相关性不佳;(2)各器官组织细胞核DNA染色强度指数均随死亡时间的延长而下降。结论(1)机体死亡后,各器官组织细胞核DNA含量随死亡时间延长逐渐减少,具有一定的变化规律,其中以脾细胞核DNA含量的变化趋势与死亡时间最具相关性,肝、肾次之,而心最差;(2)方法学分析,图像分析技术(IAT)与流式细胞术(FCM)两种检测方法在研究组织细胞DNA含量变化方面,各有利弊,可互补不足。     

对完善治安调解的几点思考

治安调解是公安机关处理特定治安案件的一种方式,在实践中由于对适用治安调解的案件范围、调解的效力等问题存在不同认识,扩大了调解的范围,影响了治安调解的效力.即将出台的<治安管理处罚法>(草案稿用名),应严格限制适用治安调解的案件范围;明确规定治安调解选择权、治安调解效力等几个问题.     

论打造"平安工程"中的城郊结合部治安

城郊结合部的治安管理,对社会治安的稳定乃至经济的发展都产生十分重大的影响.作为治安管理主要主体的公安机关,要打造"平安工程",就要从城郊结合部这个城市治安的关键区域下手,在提高认识的基础上,建立合理有效的治安防控机制,加大力度对作为治安管理客体的城郊结合部的人、财、物(尤其是人)进行有效的管理.     

高校学生工作干部远离感的成因及对策

高校学生工作干部的远离感是一个值得教育工作者关注的问题,它的存在使高校学生思想政治工作力量受到削弱,给高等教育带来了巨大危害。远离感的产生主要由高等教育体制改革、市场经济负面影响、传统的教育观念、干部本身的思想和行为方式等造成。有效控制和消除学生工作干部的远离感要更新观念,抓住"三个环节",实现"三个转变",做到"三个贴近",不断提高学生工作干部素质。     

法治政府的催化剂——行政许可法

行政许可法的出台,无疑是我国"政治文明建设的又一座里程碑",它标志着我国政府正加速从管制型政府向法治型政府转变。它的颁布、施行,将对我国推行依法行政,从源头上预防和治理腐败产生积极的影响和作用,也必将对我国行政机关政府职能的转变带来一次革命性的变化。它表明,我国"法治政府"建设正向纵深发展。     

A case study on forensic polymer analysis by DIOS-MS: the suspect who gave us the SLIP

New technology was used to identify traces of a commercial barrier/spermicide in evidence from a case of a man accused of rape of a minor. Examination of vaginal swabs performed by another laboratory had been negative for seminal fluid or other sources of DNA from the suspect and we were asked to examine the remaining swabs for any traces that might have originated from the commercial product. Encare consists of vaginal inserts having a suppository-like shape. They contain the spermicide, nonoxynol-9, in a matrix consisting of approximately two parts polyethylene glycol (PEG) 1000 to one part PEG 1450, plus minor inorganic components added to produce foaming. Portions of the cotton from vaginal swabs from the victim and penile swabs from the suspect were extracted with methanol and subsequently examined by desorption ionization on silicon time-of-flight mass spectrometry (DIOS TOF MS). Low levels of PEG in the same mass range as Encare were found on two separate vaginal swabs from the victim and one penile swab from the suspect. Subsequent to these findings, the suspect (through his attorneys) provided us with a sample of SLIP Plus, a commercial sexual lubricant that also contains nonoxynol-9. Traces of PEG in the same mass range as Encare were found in this sample, while no PEG was found in a sealed sample of SLIP Plus provided by the manufacturer. At trial the suspect's attorneys stipulated that their client had added some Encare to the SLIP Plus sample he had provided.     

简论消费者获知权

我国<消费者权益保护法>规定了消费者的获知权,它是基于对消费者弱势地位而给予的特别保护,是消费者享有的九项权利之一.消费者获知权与消费者知情权仅一字之差,但二者法律涵义不同.保护消费者合法权益已成为时代潮流,消费者获知权的实现需要国家及其各种舆论工具、消费者权益保护机构、生产经营者和消费者本人的共同努力.     

Hair analysis for ketamine and its metabolites

A rapid and sensitive method was developed for the simultaneous identification and quantitation of ketamine (K) and its major metabolite, norketamine (NK) in hair. After decontamination, the hair sample was incubated and extracted, and analyzed by gas chromatography-mass spectrometry (GC-MS). Limits of quantitation were found to be 0.05 ng/mg. Hair segments in black color were collected from 15 K abusers. Based on an experiment with 15 cavies with black, white, and brown hair, the mechanism of incorporation of K into hair was investigated. After shaving hair on the back of the cavies (8 cm x 4 cm), they were separated into three groups and administered intraperitoneally once a day for 7 successive days with high, medium, and low doses of K, respectively. Two days after this, hair segments with different colors were shaved. There was a direct correlation between the concentration of K in cavy hair and the dose and DHNK was detected only in high dosage group. The concentration of K increased in the order of white, brown, and black hair. The possible factors responsible for the incorporation of K and its metabolites in hair were discussed.     

解析行政规则对司法的约束力 以行政诉讼为论域

<正>一、引言:问题与思路由于近代民主政治理论和分权学说的功劳,法院裁断纠纷当受立法机关制定的"法律"之约束,基本上成为法学的一项公理。然而,自20世纪上叶"行政国家"兴起之后,大量整饬社会秩序的规则,直接出自行政机关而非立法机关之手,已是勿庸置疑、难以扭转的事实。于是,一个不可回避的问题应运而生:行政机关的合法角色(legitimate role)是执法者而非立法者,其制定的行政规则,对于法院而言,是否具有约束效力呢?答案     

大鼠毒鼠强中毒内脏器官Caspase-3与Bcl-2蛋白的表达

目的探讨大鼠毒鼠强中毒的病理机制。方法SD大鼠60只,随机分正常对照组、空白对照组、高剂量中毒死亡组、低剂量中毒组,高剂量中毒死亡组以1.0mg/kg毒鼠强悬浊液灌胃,低剂量中毒组以0.1mg/kg体重毒鼠强悬浊液灌胃。用免疫组织化学技术对中毒大鼠器官检测caspase-3与Bcl-2蛋白的表达,用图像分析技术对检测结果进行分析。结果各器官caspase-3与Bcl-2蛋白的表达变化规律基本相似,即高剂量中毒组大鼠caspase-3与Bcl-2蛋白均呈较强的阳性表达,低剂量中毒组大鼠,Bcl-2与caspase-3于中毒30min后表达不明显,3h时,可见较为明显的阳性信号,Bcl-2在6h～3d达高峰,随后表达下降;caspase-3在24h～5d达峰值,随后下降。Bcl-2峰值早于caspase-3出现。结论Bcl-2与caspase-3参与了毒鼠强中毒的病理生理过程。