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Loss of heterozygosity detected in a short tandem repeat (STR) locus commonly used for human DNA identification 总被引:3,自引:0,他引:3
Rubocki RJ Duffy KJ Shepard KL McCue BJ Shepherd SJ Wisecarver JL 《Journal of forensic sciences》2000,45(5):1087-1089
Short tandem repeat (STR) markers are commonly used in basic genetic research and in human identification testing. Clinically, STRs can be used to study genetic alterations in tumors. A genetic deletion common to many types of cancer is referred to as the loss of heterozygosity (LOH). Numerous examples of LOH in cancer have been described and some have been mapped to areas located in close proximity to markers employed in human identity testing. Despite this fact, LOH has rarely been observed for STR loci commonly employed in forensic testing. Recently, for medico-legal purposes, we were asked to determine whether a tissue biopsy originated from a particular individual. For a reference source we assessed two specimens, one from normal tissue and one from cancerous tissue. When both reference specimens were used to generate DNA profiles, we observed LOH at one STR locus, D13S317. As demonstrated in other cancers only the cancerous biopsy demonstrated LOH. The forensic community should be cognizant of these unusual circumstances because, as identification of human DNA continues to be used more extensively, certain instances will arise in which reference material will not be readily available. In these situations, archived specimens may be employed as a reference source. Clinical specimens such as tissue biopsies should be used with caution if they have not been confirmed to contain normal tissue. 相似文献
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Lois?A.?Ventura Eric?G.?LambertEmail author Michael?Bryant Sudershan?Pasupuleti 《American Journal of Criminal Justice》2004,28(2):165-180
The criminal justice system is supposed to protect and serve all members of the community equally; therefore, any prejudicial attitudes harbored by criminal justice students may negatively impact their job performance as future justice professionals. Four hundred and eighty-four students at a large Midwestern university were surveyed on their views toward gay and lesbian persons and issues. The responses of criminal justice majors were compared to the responses of students majoring in other fields. Criminal justice majors tended to have more negative views of gays and lesbians than students in other majors. However, there was not a significant difference between the two groups of students in their willingness to extend rights to and socialize with gay and lesbian individuals. 相似文献
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Benner BA Goodpaster JV DeGrasse JA Tully LA Levin BC 《Journal of forensic sciences》2003,48(3):554-563
This paper discusses results of a supercritical fluid extraction-gas chromatography/mass spectrometry (SFE-GC/MS) study of small samples ( 100 microg to 1 mg) of human scalp hair. The method offers a number of benefits including greater sensitivity than liquid extraction methods because the entire extractable mass is transferred to the analytical system, compared with only a few percent from a conventional liquid extraction/injection. The project's goals were to determine if SFE-GC/MS analyses of the surface-extractable components of an individual's hair yield consistent chemical profiles and to investigate if the profiles are sufficiently different to distinguish them from those of other individuals. In addition, the mtDNA sequences from ten of the same individuals used in the SFE-GC/MS study from four family units were determined, and, while the families were distinguishable, the maternal relations yielded identical sequences. In tandem, SFE-GC/MS and mtDNA techniques may provide valuable complementary data from forensic hair samples. 相似文献
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Daniel E. Hall Lois Ventura Eric G. Lambert 《American Journal of Criminal Justice》2007,32(1-2):116-128
Criminal justice professionals are a potential source of students for many criminal justice programs, especially those in metropolitan areas. This study explored factors that influenced the higher education decisions of 480 Northwestern Ohio criminal justice employees (e.g., municipal police officers, sheriff deputies, jail staff, and prison staff). Most indicated a desire to pursue higher education. Among those indicating an interest in earning a degree, cost and convenience were the most significant factors in their choice. The availability of evening and weekend classes, availability of a part-time curriculum, availability of financial aid/tuition reimbursement, total credit hours required for the degree, the field experience of faculty, and flexibility in admission requirements were all significant factors. Location was also very significant. Most respondents indicated that they would not travel more than thirty minutes to attend classes. 相似文献
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Robert Shepherd 《Canadian public administration. Administration publique du Canada》2011,54(2):277-304
Abstract: One of the key policies arising from the Federal Accountability Act 2006 was the requirement for deputy heads to establish “department and agency audit committees.” These committees comprise retired public servants, academics and leaders from the private sector. This policy requires deputy heads to take advice from “outside” advisers for the management of their departments or agencies. This is unprecedented. The author examines the reform antecedents of this policy, key implementation considerations, and what this is likely to mean for management in the federal public service. 相似文献
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Lois Bryson 《澳大利亚女权主义者研究》1990,5(12):133-136
Suzanne Franzway, Dianne Court, R.W. Connell, Staking a Claim: Feminism, Bureaucracy and the State (Allen and Unwin) Sydney, 1989; Marian Sawer, Sisters in Suits (Allen and Unwin), Sydney, 1990; Sophie Watson (ed.) Playing the State: Australian Feminist Interventions (Allen and Unwin), 1990; Anna Yeatman, Bureaucrats, Technocrats, Femocrats: Essays on the Contemporary Australian State (Allen and Unwin) Sydney, 1990. 相似文献
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