Histomorphometric characteristic of human brain in acute alcoholic intoxication

Different brain sections were studied in 20 subjects, who died of ethanol intoxication and in 14 subjects who died of injuries of the heart and main vessels, in order to detect histological changes in the brain and for the purpose of defining spatial and quantitative ratios between cerebral tissue structures in alcoholic intoxication. Different histological, stereometric and morphometric tools were made use of. It was demonstrated that, in alcoholic intoxication, there occur severe disorders of the circulation with affection of vessels in the brain; there are also dystrophic and necrotic changes in neurocytes, glial cells and white substance. The square of neurons shrinks due to death of some of them in the cortex of hemispheres, thalamus and cerebellum. As for the medulla, they are more resistant, there, to ethanol. The diameter of capillaries in the studied brain sections diminishes due to a reduced tonus of cerebral arteries; the quantity of such vessels increases within a standard area, which is conditioned by the compensatory opening of reserve capillaries. All this can be important in dealing with issues of thanatogenesis and of forensic medical diagnosis in death of alcoholic intoxication.     

Features of extracting phenol and 4-methylphenol from aqueous solutions

Phenol and 4-methylphenol were extracted from aqueous solutions by 5 organic solvents. The degree of extraction depended on such factors as type of extracting agent, pH of aqueous phase, and water saturation of extracting agent. The necessary number of extractions for obtaining the preset volumes of studied compounds is calculated.     

Isolation of eugenol from biological material

The optimal conditions for isolation of eugenol from biological material by ethyl acetate were determined. Possible purification of the compound from coextractive substances of biomaterial on the colon with silica gel L 40/100 micro is demonstrated. Methods of thin-layer and high-yield liquid chromatography for identification and quantitative determination of eugenol are proposed.     

The retention of alkyldinitrophenol compounds in cadaveric material]

The preservation of alkyl dinitrophenol compounds in corpse liver tissue was under study. The content of these compounds was assessed after various periods of the corpse storage.     

Detection of bancol for the purpose of forensic chemical expertise

Optimal conditions for the extraction of bancol from the biological material with toluene are described. The possibility of its purification and separation from co-extracted compounds on a silicagel L column, 40/100 mcm is illustrated. Identification and quantitative determination of bancol isolated from the cadaverous liver were performed by the electron spectrophotometry technique, thin-layer chromatography, and high performance liquid chromatography using normal-phase sorbents. A method of bancol detection was adapted for the purpose of forensic medical expertise and applied for the postmortem examination of the cadaverous tissues.     

DNOC extractability from aqueous solutions

Extractability of 2-methyl-4,6-dinitrophenol from aqueous solutions by means of different organic solvents was studied. Effect of solvent nature, pH medium and extractant saturation with water was noted.     

Isolation of lambda-cyhalothrin from biological material

Dioxan is proposed as an isolating agent for isolating lambda-cytalotrin (LC) from biological material. Optimal conditions for isolating LC from cadaveric hepatic tissue with dioxan were determined and the results of the isolation were quantified.     

Extraction of nitrobenzene and its metabolite 3-aminohydroxybenzene from water solutions

The article presents the results of nitrobenzene and 3-aminohydroxibensene extraction from aqueous solutions by means of five organic solvents. The following factors influence on the extraction degree is shown: the nature of the extracting agent, aqueous phase pH, saturation of some extracting agents with water. The number of extractions for isolation of the necessary quantities of the compounds studied is calculated.     

Detection of furadan in biological fluids

Optimal conditions have been determined for furadan isolation from biological fluids by means of the mixture of solvents ethyl acetate-acetone in volume 1:1. Possible purification of the compound from coextractive substances of biomaterial on the column with silica gel L 100/160 mcm is demonstrated. IR-spectrophotometric methods and high performace liquid chromatography with a detector of photodiode matrix are proposed for identification and quantitation of furadan in blood and urine specimens.     

Characteristics of dipyridamol isolation from biological material

Acetone is proposed as an isolating agent for extraction of dipyridamolum from biological material. Optimal conditions of isolating dipyridamolum from human cadaveric liver tissue with acetone are determined and quantitative estimation of isolation results is provided.