Optimization of Human mtDNA Control Region Sequencing for Forensic Applications

Sequencing mitochondrial DNA hypervariable regions I and II (HVI and HVII) is useful in forensic missing person and unidentified remains cases. Improvements in ease and sensitivity of testing will yield results from more samples in a timely fashion. Routinely, amplification of HVI and HVII is followed by Sanger sequencing using the BigDye^® Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) using 4 μL of ready reaction mix (RRM). Each sequencing reaction is then purified through column filtration before capillary electrophoresis. Using lower amounts of RRM (2 μL or 1 μL) and purification using BigDye^® XTerminator^? (Applied Biosystems) instead of columns showed no loss of sequence length and increased the quality and the sensitivity of testing, allowing HVI and HVII typing from mitochondrial genome equivalent to 125 fg of nuclear DNA, or 100 pg of HVI/HVII amplicons. Using this methodology, testing can be completed in 1 day, and the cost of testing is reduced.     

Moderated Commitment: Members’ Decisions Not to Give to Professional Associations

Abstract

Workers pay membership dues to professional associations, which mark their identification with a particular field. Professional associations invite additional voluntary contributions for new or expanded programs, but most members do not make these additional gifts. We advance and test a conceptual model of the forces that might interrupt an otherwise committed or engaged member’s decision to make such a voluntary contribution. We conclude that giving decisions can be displaced by member beliefs that their dues and fees are sufficient or that their disposable income is committed to other obligations. We also conclude that giving decisions intersect with weak association strategy, such as a failure to solicit gifts in ways that are informative or useful to prospective donors.     

Identification and Individualization of Lophophora using DNA Analysis of the trnL/trnF Region and rbcL Gene

Lophophora williamsii (peyote) is a small, spineless, greenish‐blue cactus found in Mexico and the southwestern United States. Ingestion of the cactus can result in hallucinations due to its content of mescaline. In the United States, L. williamsii is classified as a Schedule I controlled substance. In this study, we use DNA analysis of the chloroplast trnL/trnF region and chloroplast rbcL gene to identify the individuals of Lophophora. Using the rbcL gene, Lophophora specimens could be distinguished from outgroups, but species within the genus could not be distinguished. The trnL/trnF region split the Lophophora genus into several groups based on the length and substructure of an AT‐rich segment of the sequence. Our results indicate that the genetic variability at the trnL/trnF locus is greater than previously recognized. Although DNA structures at the trnL/trnF region and rbcL gene do not align with the classification of Lophophora species, they can be used to aid in forensic analysis.     

TOF‐SIMS Analysis of Red Color Inks of Writing and Printing Tools on Questioned Documents

Time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) is a well‐established surface technique that provides both elemental and molecular information from several monolayers of a sample surface while also allowing depth profiling or image mapping to be performed. Static TOF‐SIMS with improved performances has expanded the application of TOF‐SIMS to the study of a variety of organic, polymeric, biological, archaeological, and forensic materials. In forensic investigation, the use of a minimal sample for the analysis is preferable. Although the TOF‐SIMS technique is destructive, the probing beams have microsized diameters so that only small portion of the questioned sample is necessary for the analysis, leaving the rest available for other analyses. In this study, TOF‐SIMS and attenuated total reflectance Fourier transform infrared (ATR‐FTIR) were applied to the analysis of several different pen inks, red sealing inks, and printed patterns on paper. The overlapping areas of ballpoint pen writing, red seal stamping, and laser printing in a document were investigated to identify the sequence of recording. The sequence relations for various cases were determined from the TOF‐SIMS mapping image and the depth profile. TOF‐SIMS images were also used to investigate numbers or characters altered with two different red pens. TOF‐SIMS was successfully used to determine the sequence of intersecting lines and the forged numbers on the paper.     

Garment, or Upper-Garment? A Matter of Interpretation?

In an adversarial common law courtroom, where one party tries to defeat the other by using words as weapons, polysemous words more often than not pose a problem to the court interpreter. Unlike in dyadic communication, where ambiguity can be easily clarified with the speaker by the hearer, court interpreters’ freedom to clarify with speakers is to a large extent restricted by their code of ethics. Interpreters therefore can only rely on the context for disambiguating polysemous words. This study illustrates the problem of polysemy in an interpreter-mediated rape trial. It exemplifies how the interpreter’s goal to avoid contradictions by making her interpretation of a polysemous word consistent with the preceding context runs counter to that of the bilingual cross-examiner, whose primary goal is to identify inconsistencies in the hostile witness’s testimony in order to discredit him. This study also manifests a denial of the interpreter’s latitude in the interpretation of contextual clues and her loss of power in a courtroom with the presence of other bilinguals.     

Production networks in China and India: a comparative analysis

In this paper, we examine and compare the two important production hubs in Asia: China and Greater China and India and South Asia. We show that in China, Hong Kong and Taiwan, manufacturing trade has continued to be highly relevant, with trade in parts and components growing in importance. In contrast, in India, Pakistan and Sri Lanka, trade in parts and components remain limited. We then calculate various revealed comparative advantage (RCA) indices, which China having more components with RCA values exceeding one. Some of the most important components exported by China include electronic parts and telecommunication parts. One explanation is to why India is relatively weak in parts and components trade is that India is strong in service trade. Other reasons may be related to better port infrastructure, higher research and development intensity and higher educational enrollments in China.     

Hong Kong's Elite Structure,Legislature and the Bleak Future of Democracy under Chinese Sovereignty

A decade after retrocession to China, Hong Kong still has limited democracy. Only half of the legislature is directly elected by the people, and the Chief Executive – the leader of Hong Kong government – is chosen by an election committee of 800 people, most of them loyal to Beijing. In December 2007, the Chinese Central Government announced that the direct election of the Chief Executive and the whole legislature may be implemented from 2017 and 2020, respectively. Still, in this paper it is suggested that real democracy in the foreseeable future is unlikely. This paper demonstrates the existence of a “power elite” of tightly knit business-state networks in Hong Kong society and argues that this constitutes a systemic barrier against further democratic development. Central to the discussion is an analysis of the formation and composition of the legislature and the relations to the elite-interlocks among influential organisations in society. Mainly based on 2006‐07 data, the Elite Database which is subject to network analyses is composed of 1531 individuals holding 1854 seats from 40 listed corporations, 27 government committees, 20 non-governmental organisations, and eight universities.     

From Polarisation to Integration in Vietnam

This paper analyses the growth trajectory of China and related structural change to assess China's capacity to continue its rapid growth over the next decade. The evidence demonstrates that the multi-path approach undertaken has enabled China to transform its economy from low value-added towards high value-added activities through structural change from low to high value-added industries, as well as upgrading within industries. In doing so, China did not follow the neo-liberal advocacy of freeing markets. Selective state interventions facilitated China's transformation from an agricultural to an industrial economy over the last few decades. Upgrading towards higher value-added activities and the continuing strength of macroeconomic indicators, such as balance of payment and capital account surpluses, and low trade intensity of GDP and debt service along with significant deepening in human capital and R&;D activities, suggests that China will continue to grow relatively rapidly over the next decade. To do this China needs to find solutions to growing deficits in power and water supply, and potentially dangerous political upheavals if growing economic inequality problems are not solved.     

Controlled electrostatic methodology for imaging indentations in documents

The electrostatic process for imaging indentations on documents using the ESDA device is investigated under controlled experimental settings. An in-house modified commercial xerographic developer housing is used to control the uniformity and volume of toner deposition, allowing for reproducible image development. Along with this novel development tool, an electrostatic voltmeter and fixed environmental conditions facilitate an optimization process. Sample documents are preconditioned in a humidity cabinet with microprocessor control, and the significant benefit of humidification above 70% RH on image quality is verified. Improving on the subjective methods of previous studies, image quality analysis is carried out in an objective and reproducible manner using the PIAS-II. For the seven commercial paper types tested, the optimum ESDA operating point is found to be at an electric potential near -400V at the Mylar surface; however, for most paper types, the optimum operating regime is found to be quite broad, spanning relatively small electric potentials between -200 and -550V. At -400V, the film right above an indented area generally carries a voltage which is 30-50V less negative than the non-indented background. In contrast with Seward's findings [G.H. Seward, Model for electrostatic imaging of forensic evidence via discharge through Mylar-paper path, J. Appl. Phys. 83 (3) (1998) 1450-1456; G.H. Seward, Practical implications of the charge transport model for electrostatic detection apparatus (ESDA), J. Forensic Sci. 44 (4) (1999) 832-836], a period of charge decay before image development is not required when operating in this optimal regime. A brief investigation of the role played by paper-to-paper friction during the indentation process is conducted using our optimized development method.     

Environmental management and street-level regulators: A cultural trap?

It has been argued that environmental management is in an administrative trap. Numerous factors leading to this trapped situation have already been identified in the literature: institutional rigidity, lack of co-ordination, formalization, non-accountability and political interference. This study focusses on the organizational, cultural dimension of the administrative trap and argues that in order to reform administrative structures, the organizational culture must also be reformed. On the basis of several face-to-face interviews with street-level environmental regulators in Hong Kong, the study claims that a role-oriented, hierarchical culture exists. While such an organizational culture is in perfect agreement with existing administrative arrangements, it may, in the end, hamper the effectiveness of environmental management.