Developmental validation of the SPERM HY-LITER™ kit for the identification of human spermatozoa in forensic samples

Abstract: With sexual assault evidence, the visualization of spermatozoa confirms that ejaculation has occurred. However, microscopic examination of spermatozoa is a laborious process and can sometimes result in sperm cells being overlooked. Here, we present the developmental validation of the SPERM HY‐LITER? kit, which contains a human sperm–specific mouse monoclonal antibody coupled to a fluorescent Alexa 488 dye. The kit was tested using samples of human semen, saliva, blood, and urine, various animal semen extracts, sexual lubricants, and a commercially available spermicidal film. Postcoital vaginal swabs, degraded semen samples, and samples prepared with sample fixation techniques that deviated from the kit‐provided protocol were also tested. In each case, the SPERM HY‐LITER? kit was demonstrated to bind only to human sperm cell heads. Limitations to this fluorescent staining procedure include nonspecific staining and increased background fluorescence with extreme heat fixation in some samples.     

Fragrance transfer between fabrics for forensic reconstruction applications

Sexual assault is a serious crime that often has low conviction rates. Recent literature has demonstrated that there is potential for fragrances to be valuable in forensic reconstructions where there has been contact between individuals. However, developing appropriate evidence bases for understanding the nature of fragrance transfer in these contexts is needed. This article presents three experiments that address the transfer process of fragrances that have been transferred from a primary piece of fabric onto a secondary piece of fabric, in a manner that could occur during an assault. The three variables studied were the ageing time of the fragrances on the first fabric prior to transfer, the contact time between the two fabrics, and lastly the fabric type (of the primary material and the recipient material). The transfer was evaluated using a validated solid phase micro-extraction gas chromatography–mass spectrometry (SPME GC–MS) method. The findings demonstrated that all three variables had an impact on the transfer of fragrances between clothing fabrics. Generally, lower volatility compounds were transferred and recovered in larger amounts than higher volatility compounds. All fragrance compounds were successfully recovered from a secondary piece of fabric even when the contact time was as short as 10 s, and even when the perfume was aged on the primary fabric for as long as 48 h. The nature of the fragrance transfer also depended on the fabric type, so that a clear discrimination was observed between the fragrance transfer that occurred onto a natural fabric (cotton) and onto a synthetic fabric (polyester).     

Assessment of exposure to opiates and cocaine during pregnancy in a Mediterranean city: preliminary results of the "Meconium Project"

For the first time in Europe, the "Meconium Project" aimed to estimate the prevalence of drug use by pregnant women and the effects of exposure to illicit drugs during pregnancy on the fetus and infant. Between October 2002 and February 2004, 1151 (79%) dyads among the 1439 mother-infant dyads from the Hospital del Mar, Barcelona, Spain, met eligibility criteria and agreed to participate in the study. We present preliminary results on the first 830 meconium samples and 549 mother-infant dyads, for which statistical analysis of socio-economic and demographic characteristics and newborn somatometry was completed. The meconium analysis showed an overall 7.9% positivity for drugs of abuse, with 6-monoacetylmorphine and cocaine being the analytes, most frequently found in samples positive for opiates and cocaine. Structured interview disclosed 1.3, 1.8 and 1.3% of mothers exposed to opiates, cocaine and both drugs, while only one mother declared ecstasy consumption. Meconium analysis showed that prevalence of opiates, cocaine and combined drugs exposure was 8.7, 4.4 and 2.2%, respectively, and confirmed the case of ecstasy use. Arecoline, the main areca nut alkaloid, was found in meconium specimens from four Asiatic newborns, whose mothers declared beetle nut consumption during pregnancy. Parental ethnicity was not associated with drug use, nor was the social class, although a higher tendency toward drug consumption was observed in professional and partly skilled mothers. Drug consuming mothers showed a higher number of previous pregnancies and abortions (p<0.05) when compared to non-consumer mothers (meconium negative test), probably due to a lack of family planning. Consumption of opiates and cocaine during pregnancy was associated with active tobacco smoking, a higher number of smoked cigarettes and cannabis use. Exposure status and smoking behavior correlated with significantly lower birth weight in newborns from mothers exposed only to cocaine and to opiates and cocaine simultaneously. Of the four newborns exposed to arecoline, one showed a low birth weight, low intrauterine growth, hyporeflexia and hypotonia.     

Financing knowledge-intensive enterprises: evidence from CVCs in the US

Since the 1990s, the importance of corporate venture capital (CVC) programs has grown around the world. CVCs are investments that established firms make in entrepreneurial companies. At the most basic level, CVC describes an equity investment made by a corporation or its investment entity in a high growth, high potential, privately held business. There is no systematic evidence that corporate venture capital investments create value for the investing firms. Firm value, however, can be created as a result of other benefits from investing (e.g., accessing a new technology). These considerations can explain why many firms currently choose to operate venture units: They have recognized the importance of CVC for strategic innovation in addition to its potential to generate financial returns. Some evidence from the US context described in this paper supports this intuition.     

Social web knowledge sharing and innovation performance in knowledge-intensive manufacturing SMEs

This paper develops an integrative research model to assess the effect of different factors on social web knowledge sharing and its effect on innovation performance in manufacturing small and medium-sized enterprises (SMEs). In addition, this study analyzes whether social web knowledge sharing may be a mediator in the relationship between human resource (HR) practices and innovation performance. The proposed research model and its associated hypotheses were tested by using partial least squares structural equation modeling on a dataset of manufacturing SMEs. This study contributes to research seeking to understand the factors affecting social web knowledge sharing by demonstrating that technological and organizational factors have greater impact than environmental factors on social web knowledge sharing. It also contributes to research by exploring the indirect effects of the social influence of HR practices on organizational innovation performance by offering evidence on the mediating effect of social web knowledge sharing in the relationship between HR practices and organizational innovation performance in manufacturing SMEs.     

Statistical analysis of DNA mixtures using peak area information and allelic drop out

A case of a woman killed in Perugia is reported. The woman was beaten to death and the body showed evidence of bites, kicks and punches. The request of the Court was to verify the presence of bites and if they belonged to humans. Morphological examination and genetic analysis with human Y-specific markers were performed in order to verify the origin of the bites. The DNA profile from the surrounded area of the traces was compared with the profile of the victim's husband (the suspect).The results showed a match between the profile of the suspect and that of the traces for all loci examined. Due to the fact that also other relatives of the husband's male lineage lived in the same house, it was not possible to identify the man who really contributed to the traces. Therefore, the analysis was implemented with autosomal STR markers, which showed a mixed genetic profile. In order to verify the number and the identity of the contributors, statistical analysis based upon peak area information was performed with Probabilistic Expert Systems.     

The Motivated Processing of Political Arguments

We report the results of an experiment designed to replicate and extend recent findings on motivated political reasoning. In particular, we are interested in disconfirmation biases—the tendency to counter-argue or discount information with which one disagrees—in the processing of political arguments on policy issues. Our experiment examines 8 issues, including some of local relevance and some of national relevance, and manipulates the presentation format of the policy arguments. We find strong support for our basic disconfirmation hypothesis: people seem unable to ignore their prior beliefs when processing arguments or evidence. We also find that this bias is moderated by political sophistication and strength of prior attitude. We do not find, however, that argument type matters, suggesting that motivated biases are quite robust to changes in argument format. Finally, we find strong support for the polarization of attitudes as a consequence of biased processing.

The League of Polish Families between East and West,past and present

Historical legacies play an important role in the rise of radical right parties in Central and Eastern Europe. This article conducts an in-depth study of the trajectory of a particular radical right party, the League of Polish Families, in a particular Central and East European country, Poland. The central objective of the article is to highlight that, although there are important similarities between the League of Polish Families and other radical right parties in both Central and Eastern Europe and Western Europe, the League of Polish Families differs in some respects, such as the composition of electorate and ideology from these parties. The article shows that the observed differences have their roots in the Polish historical legacy, that on some accounts deviates from the historical legacies present in other Central and East European countries.     

Peer Influences on Moral Disengagement in Late Childhood and Early Adolescence

Moral disengagement processes are cognitive self-justification processes of transgressive actions that have been hypothesized to be learned and socialized within social contexts. The current study aimed at investigating socialization of moral disengagement by friends in two developmentally different age groups, namely late childhood (age: 9–10 years; n = 133, 42.9 % girls) and early adolescence (age: 11–14 years; n = 236, 40.6 % girls) over a 1-year period. Specifically, the current study examined whether similarity in moral disengagement between friends was the result of friends’ influence or friend selection. Moreover, gender (42 % girls), individual bullying behavior, and perceived popularity status were examined as potential moderators of socialization for moral disengagement within friendship networks. Self-report measures were used to assess moral disengagement, sociometric questions and a peer-nomination scale for friendship networks and bullying behavior, respectively. Longitudinal social network analysis (RSiena) was used to study change of moral disengagement in friendship networks during a 1-year interval. In early adolescence, friends were more likely to be similar to each other over time and this was explained only by influence processes and not by selection processes. Gender, bullying, and perceived popularity did not moderate the friends’ influence on moral disengagement over time. Results indicate that self-justification processes change over time already in late childhood, but only in early adolescence this change is likely to be dependent upon peers’ moral disengagement.