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The use of Qiagen QIAamp and QIAshredder for extracting DNA from envelope flaps and stamps is reported. The stamps or flaps can be added directly to extraction solutions and the DNA is bound to a spin column containing a silicon membrane for washing prior to elution. DNA extracted from four stamps and four envelope flaps was amplified and analyzed using a multiplex PCR system. Complete DNA profiles were obtained from five samples. 相似文献
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The role of prosecution in achieving compliance with social regulation is a highly contentious issue, nowhere more so than with regard to work-related injury and death in the New South Wales mining industry. Following a mining disaster, political pressure prompted the mines inspectorate to abandon its traditional 'advise and persuade' approach in favour of a much tougher, deterrence-oriented approach. Our field-work suggests that while the former approach can result in regulatory capture, the latter can be equally counterproductive. In the mining industry, interactions between inspectors and the regulated industry are frequent and ongoing and trust is central to constructive relations. When those relations break down (as under an inappropriate prosecution policy) then dialogue ceases, information is withheld rather than shared, in-firm accident investigation, prevention, and remedial action are inhibited and both sides retreat to a form of adversarialism that undermines regulatory effectiveness. Through a 20-year case study of the mines inspectorate, the article demonstrates the centrality of trust to regulatory effectiveness, how it can be lost, and how it can best be regained. 相似文献
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The term 'sovereignty' figures prominently in international affairs and academic analysis. But does 'sovereignty' mean the same thing in different countries and political cultures? In this article, we examine conceptions of sovereignty as they appear in the writings of US scholars of international law and those international relations scholars who deal with international law, in order to obtain a clearer picture of what 'sovereignty' means in American academic discourse. At first glance, the US literature is dominated by two distinct conceptions of sovereignty: (1) a statist conception that privileges the territorial integrity and political independence of governments regardless of their democratic or undemocratic character; (2) a popular conception that privileges the rights of peoples rather than governments, especially when widespread human rights violations are committed by a totalitarian regime. On closer examination, what seem to be two conceptions are in fact different manifestations of a single, uniquely American conception of sovereignty which elevates the United States above other countries and protects it against outside influences while concurrently maximising its ability to intervene overseas. 相似文献
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Stephen Sinclair 《Local Government Studies》2013,39(1):77-92
A change in the cost structure in the Danish municipalities seems to have occurred bringing in a larger fixed element in the cost function and making unit costs higher in small municipalities than in large. The paper examines the hypothesis that small municipalities in their reaction to their higher unit costs for management make a trade-off between raising expenditures and the tax level, on the one hand, and reducing the quality of the production, on the other. Using accounting data for 1980, 1990 and 2000 it is shown that the expenditure structure has shifted since 1980 so that small municipalities now exhibit higher unit expenditures than larger municipalities. However, there also seems to be a quality substituting effect in the small municipalities as a reaction to the higher unit costs. From data based on a questionnaire to the chief executives in all municipalities indications of weak political and administrative leadership in the small municipalities are demonstrated. We find that population size and administrative capacity explain significant differences in municipal understanding of the consequences of the central government regulation defining the conditions for the local production of services. 相似文献