Inferring ancestral origin using a single multiplex assay of ancestry-informative marker SNPs

Tests that infer the ancestral origin of a DNA sample have considerable potential in the development of forensic tools that can help to guide crime investigation. We have developed a single-tube 34-plex SNP assay for the assignment of ancestral origin by choosing ancestry-informative markers (AIMs) exhibiting highly contrasting allele frequency distributions between the three major population-groups. To predict ancestral origin from the profiles obtained, a classification algorithm was developed based on maximum likelihood. Sampling of two populations each from African, European and East Asian groups provided training sets for the algorithm and this was tested using the CEPH Human Genome Diversity Panel. We detected negligible theoretical and practical error for assignments to one of the three groups analyzed with consistently high classification probabilities, even when using reduced subsets of SNPs. This study shows that by choosing SNPs exhibiting marked allele frequency differences between population-groups a practical forensic test for assigning the most likely ancestry can be achieved from a single multiplexed assay.     

Looking Ahead: Addressing Ethical Challenges in Public Health Practice

Ethical challenges in public health can have a significant impact on the health of communities if they impede efficiencies and best practices. Competing needs for resources and a plurality of values can challenge public health policymakers and practitioners to make fair and effective decisions for their communities. In this paper, the authors offer an analytic framework designed to assist policymakers and practitioners in managing the ethical tensions they face in daily practice. Their framework is built upon the following set of six considerations: determining population-level utility of the proposed action; demonstrating evidence of need and effectiveness of actions; establishing fairness of goals and proposed implementation strategies; ensuring accountability; and, assessing expected efficiencies and costs associated with the proposed action. Together, these considerations create a structured guide to assist decision-makers in identifying potential ethical challenges and in assessing the moral considerations that underlie public health practice - and possibly even, if the conditions are met, reduce the creation of ethical tension. Although the authors'empirical experiences provide the basis for the framework advanced here, their approach remains to be tested and evaluated by public health practitioners.     

The Search for Job Satisfaction: A Survey of Alabama Policing

This study examines police job satisfaction and its association with general occupational attraction. Police officers from sixteen municipal departments (n = 1,114) across one southern state (Alabama) were found to possess moderate-high overall job satisfaction, with regression analysis supporting the conclusions of prior studies that demographics are of little value to understanding job satisfaction. The findings convey that 25% of job satisfaction variance can be explained from six variables (in order of importance): social contribution, pay, adventure/excitement, autonomy, peer respect, and job security. The importance of benefits and community respect was unsupported, as were partner and special operation assignments. The author concludes that a great portion of police satisfaction hinges on the realization of work environment expectations, and that departments must proactively construct policies to enhance fulfillment of officer aspirations.     

Nothing to Lose: An Insurance Company's Duty of Good Faith and Fair Dealing,Punitive Damages,and The Legacies of Campbell

This article explores the potential evisceration of the deterrence function of punitive damages, one of the only economic incentives that deters insurance companies from opportunistically breaching their insurance policies. Readers will gain an understanding of why large punitive damages awards are essential in the insurance coverage field and how such awards may implicate due process standards. The article explains the constitutional limitations set forth by the U.S. Supreme Court in imposing punitive damages, and provides a roadmap of how lower courts and practitioners are presently applying these rules.     

Patterns in the destruction of the ribs

The article deals with study of some patterns of long spongy rib bone destruction in static and dynamic load of thorax. Model of bone destruction is suggested. Multiple microfissures preceding formation of complete bone destruction were detected in border areas with fracture. They have peculiar mosaic depending on load conditions. Microfissure localization in relation to osteon structure of rib cortical lamina was stated. The results of investigations may have theoretical and applied value both in medicolegal and clinical traumatology.     

Computer-assisted identification of basic substances

The potential applications of a personal computer with integrated database software in toxicologic analysis are discussed. A toxicologic database including 370 basic compounds, is presented that was built up without knowledge of a higher programming language and was used as a base for drug identification in normal analysis by PC, DC and GC.     

‘Robust and Raring to Go?’– Judges' Perceptions of Child Witnesses

This article explores judicial perceptions of child witnesses. It considers the impact of recent legislation in England and Wales as well as in Scotland which classified all child witnesses as vulnerable and introduced a series of special measures to facilitate children's evidence. The article reports the findings of an empirical research study conducted with the judiciary in Scotland which suggests that judicial perceptions of child witnesses extend across a complex spectrum where a child may be viewed as vulnerable but is also likely to be seen as suggestible, reliable or resilient. The article advances two propositions. First, that the statutory conceptualization of children as invariably vulnerable has not displaced established beliefs concerning children's suggestibility and therefore has made little difference to perceptions of their ability to produce reliable testimony. Second, that focusing on children's potential for resilience rather than their vulnerability may prove a more productive conceptualization of children, one which could better support their capability as witnesses.     

Successful DNA typing of ultrafiltered urines used to detect EPO doping

Endogenous and exogenous erythropoietin (EPO) present in urine can be distinguished according to their isoelectric profiles. This methodology requires urine samples to be concentrated about 200 to 1000 times with manipulations that should remove most of the cells occurring in the original sample. In this study, we tried to obtain DNA profiles from 10 ultrafiltered urines (retentates) in order to evaluate whether a formal genetic identification was technically feasible. No nuclear DNA profiles could be established from retentates, despite 34 PCR-cycles amplifications. Contrastingly, mitochondrial DNA (mtDNA) profiles were obtained for 9 out of the 10 retentates. Apart from some particularities, retentate mtDNA profiles were all distinct and matched mtDNA profiles of corresponding reference samples.     

Factors Influencing the Higher Education Decisions of Criminal Justice Professionals

Criminal justice professionals are a potential source of students for many criminal justice programs, especially those in metropolitan areas. This study explored factors that influenced the higher education decisions of 480 Northwestern Ohio criminal justice employees (e.g., municipal police officers, sheriff deputies, jail staff, and prison staff). Most indicated a desire to pursue higher education. Among those indicating an interest in earning a degree, cost and convenience were the most significant factors in their choice. The availability of evening and weekend classes, availability of a part-time curriculum, availability of financial aid/tuition reimbursement, total credit hours required for the degree, the field experience of faculty, and flexibility in admission requirements were all significant factors. Location was also very significant. Most respondents indicated that they would not travel more than thirty minutes to attend classes.