Acidic and neutral drugs screen in blood with quantitation using microbore high-performance liquid chromatography–diode array detection and capillary gas chromatography–flame ionization detection

Blood previously acidified with aqueous saturated ammonium chloride solution was extracted with ethyl acetate. The dried extract was subjected to acetonitrile–hexane partition. The acetonitrile portion was analysed for the presence of acidic and neutral drugs by HPLC–DAD (200 mm×2.1 mm I.D. microbore ODS-Hypersil column) and GC–FID (25 m narrow-bore×0.25 mm I.D. HP-5 column with 0.33 μm film thickness). The protocol was found to be suitable for both clinical toxicology (including emergency toxicology) and postmortem toxicology. At least 66 drugs of interest were unequivocally identified by RRTs (HPLC) and UV spectra (DAD) match while another 12 were unequivocally identified by double RRTs match (HPLC and GC). Quantitation was facilitated by incorporating calibration blood standards in each assay batch. The five drugs most commonly encountered in clinical blood specimens (1150 cases) were: paracetamol (47.4% of the cases); chlormezanone (6.6%), theophylline (1.74%), naproxen (1.65%) and mefenamic acid (1.56%). The following drugs were detected in toxicologically significant quantities in postmortem blood specimens (245 cases): phenobarbitone (1.22% of the cases), naproxen (0.82%), chlormezanone (0.82%), theophylline (0.82%), carbamazepine (0.41%) and paracetamol (0.41%).     

监狱警察工资法定性探析——写在《公务员法》颁布之后

监狱警察的工资问题,不仅关系警察的切身利益,关系对警察履职的警务保障,关系对警察队伍的依法管理,还关系当前监狱工作的改革成效和发展进程,因此,必须予以高度重视。本文拟围绕《公务员法》等有关法律法规,对警察工资的法定性谈一些个人见解。一、关于警察工资的法律保障警察     

执政环境研究的一个理论支撑--建立中国特色的环境政治学

中国共产党在新世纪和中国社会发展的新阶段,提出加强党的执政环境研究的命题,提出并构建中国特色的环境政治学,是对我们党加强执政环境建设研究的积极回应,具有重大的理论价值和现实意义.     

领导干部的社会主义荣辱观

胡锦涛同志提出的社会主义荣辱观,涵盖了爱国主义、集体主义和社会主义的丰富内容,指明了社会主义市场经济条件下人们世界观、人生观、价值观的核心所在,明确了当代中国的道德评价体系和道德评价标准。高屋建瓴,切中时弊,具有强烈的现实针对性。各级领导干部应准确把握社会主义荣辱观的内涵,充分认识树立社会主义荣辱观的必要性,为全社会作出表率。     

对农村学习贯彻“三个代表”重要思想的思考——哈密市乡村“三个代表”学习教育活动的几点体会

在农村开展学习贯彻“三个代表”重要思想,必须注重实效,必须把学教活动与农民增收紧密结合起来,切实转变干部作风,建设一支高素质的农村干部队伍。     

论提高新闻工作者的科技素质

我国现时的科技宣传工作 ,对新闻工作者的科技素质提出了更高的要求。按照舆论的超前原则 ,提高新闻工作者的科技素质 ,已成为当前新闻队伍建设亟待解决的重要问题之一。     

A case of sudden infant death due to massive hemorrhage in primitive neuroectodermal tumor.

A case of the sudden death of a 14-month-old girl due to massive hemorrhage in a primitive neuroectodermal tumor (PNET) is presented along with a review of the relevant literature. PNET is a rare, malignant brain neoplasm occurring predominantly in children.     

G3M T typing by dot immunobinding with monoclonal anti-G3M T antibody

Dilutions of 100 serum samples of various GM phenotypes were dotted onto a nitrocellulose membrane. The serum dot-blots were detected with peroxidase-labeled anti-G3M T monoclonal antibody (anti-G3M T MAb). Up to a 1 : 256 dilution could be G3M T-typed correctly. By use of anti-G3M T MAb and peroxidase-labeled anti-mouse IgG or the biotin-avidin system instead of use of labeled anti-G3M T MAb, up to a 1 : 512 or 1 : 1024, respectively, dilution was typable. As with previous work with G3M G MAb, the dot immunobinding (DIB) method for G3M T typing was found very simple and practical.     

邓小平关于新时期社会政治稳定的基本思想

社会政治稳定是现代化建设顺利推进的前提条件 ,邓小平关于新时期社会政治稳定的基本思想 ,是指导我们正确处理改革、发展与稳定关系的理论基础。