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A solid-phase microextraction-gas chromatographic-mass spectrometric (SPME-GC-MS) method has been developed and validated for measuring four club drugs in human urine. These drugs include gamma-hydroxybutyrate (GHB), ketamine (KET), methamphetamine (MAMP), and methylenedioxymethamphetamine (MDMA). These drugs are referred to as 'club drugs' because of their prevalence at parties and raves. Deuterium labeled internal standards for each of the four drugs was included in the assay to aid in quantitation. The drugs were spiked into human urine and derivatized using pyridine and hexylchloroformate to make them suitable for GC-MS analysis. The SPME conditions of extraction time/temperature and desorption time/temperature were optimized to yield the highest peak area for each of the four drugs. The final SPME parameters included a 90 degrees C extraction for 20min with a 1min desorption in the GC injector at 225 degrees C using a splitless injection. All SPME work was done using a 100microm PDMS fiber by Supelco. The ratio of pyridine to hexylchloroformate for derivatization was also optimized. The GC separation was carried out on a VF-5ht column by Varian (30m, 0.25mm i.d., 0.10microm film thickness) using a temperature program of 150-270 degrees C at 10 degrees C/min. The instrument used was a ThermoFinnigan Trace GC-Polaris Q interfaced with a LEAP CombiPal autosampler. The data was collected by using extracted ion chromatograms of marker m/z values for each drug from the total ion chromatograms (TIC) (full scan mode). Calibration curves with R(2)>0.99 were generated each day using the peak area ratios (peak area drug/peak area internal standard) versus concentration. The validated method resulted in intra-day and inter-day precision (% R.S.D.) of less than 15% and a % error of less than 15% for four concentrations in the range of 0.05-20microg/mL (MAMP) and 0.10-20microg/mL (GHB, KET, and MDMA). This method has the advantage of an easy sample preparation with acceptable accuracy and precision for the simultaneous quantification of these four drugs of abuse and shows no interference from the urine matrix. 相似文献
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Purpose
To examine the persistence of physical aggression in preschoolers and associated correlates (i.e., socio-demographic, socioeconomic, criminality, parenting practices, maternal mental health).Methods
One-year follow-ups are completed with 240 mothers and their preschool children (boys and girls) from the Vancouver Longitudinal Study on the Psychosocial Development of Children. A series of structural equation models are examined.Results
Maternal psychological symptoms, juvenile delinquency, and adult offending are associated with higher levels of physical aggression in their offspring. Children of non-Caucasian mothers and those born outside of North are less physically aggressive. Cultural differences in the correlates of physical aggression were identified.Conclusions
Maternal past delinquency, current adult offending, and mental health are important factors in the development of children’s physical aggression. The findings suggest that there are multiple pathways leading to chronic physical aggression, which may be culturally-based. Cultural differences should be taken into account when developing programs and intervening with families of children with behavioral problems. 相似文献16.
Conventional models of bargaining and reassurance under incomplete information assume that actors' behavioral signals are objectively cooperative or noncooperative. Even if actors are uncertain of each other's preferences, they know what types of actions the other will view as cooperative. Yet on many real-world issues, cooperation is subjective, and what constitutes a cooperative action is conditional on the receiver's preferences. We present a formal model showing that in these cases, two-sided incomplete information actually incentivizes honest behavior and facilitates credible signaling. Because uncertain senders do not know whether a particular action will be interpreted as cooperative, they have little incentive to misrepresent, and instead honestly pursue their true goals. Thus, where cooperation is subjective, mutual uncertainty is “offsetting,” such that credible signals allow actors to quickly and accurately update their beliefs. We illustrate this logic through a case study of the Sino–Soviet split, and highlight the model's implications for contemporary U.S.–China relations. 相似文献
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Michael W. Haynes 《政治学》2000,20(2):69-76
Ulrike Marie Meinhof's televised play Bambule was not broadcast for fear that it would elicit sympathy for the terrorist organisation she founded. This is despite there being no justification of violence for political ends. The withholding of funding and sponsorship continued into the late 1980s for works that condemned violence, but which also refused to provide positive support for the anti-terrorist measures of the state. Although understandable when a society is confronted by a crisis such as terrorism, it may hinder both the understanding of the complexities of political violence and debate over the issues it raises. 相似文献
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Jeff Haynes 《Contemporary Politics》2000,6(2):123-141
This article is concerned with democratization in the Third World since the eighties, a period when dozens of Third World countries have undergone at least some democratic change. The article aims to assess the extent of democratic progress across a range of Third World countries and to compare their democratic experiences. The article examines a range of factors which have led to the development of a large number of ‘electoral' democracies which incorporate a number of democratic characteristics but exclude others. The author seeks to explain the emergence of this type of model. 相似文献
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