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Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB) 总被引:1,自引:0,他引:1
Several assays for gamma-hydroxybutyrate (4-hydroxybutyrate, GHB) have been developed based on the enzyme gamma-hydroxybutyrate dehydrogenase (GHB-DH). Enzymatic oxidation of GHB by NAD+ is coupled to diaphorase-mediated reduction of pro-dye to yield colored product. GHB-DH from Ralstonia eutropha was cloned and expressed as a stable fusion protein easily purified by affinity chromatography. Quantitative initial velocity and endpoint versions of the assay in solution are described. Michaelis-Menten parameters for oxidation of GHB and ethanol were estimated. A semi-quantitative "dipstick" version of the assay on paper also is described. Both solution endpoint and "dipstick" assays are sensitive to about 0.05 mg GHB/mL using 10 microL of sample. Ethanol at concentrations possible in urine and agents used to stabilize physiological fluids for forensics analysis do not interfere significantly. The "dipstick" assay also allows detection of GHB in alcoholic beverages after evaporation of about one-fourth drop of beverage before testing. The enzymatic assay for GHB is reliable, sensitive, inexpensive and rapid. 相似文献
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Thirty-six male students, drawn from a sample of 1195, were interviewed to obtain a personal history. A battery of projective psychological tests (Rorschach and TATs) were also administered to them. The students were divided into four groups of nine each, Jewish radicals (JR), Christian radicals (CR), Jewish moderates (JM), and Christian moderates (CM), to test the significance of religious background as it related to political outlook. Eight significant psychological variables were found and defined. No differences were found between JMs and CMs. Radicals differed from moderates on three variables: negative identity, masochistic surrender, and treating people as concepts. In addition, JR subjects demonstrated consistently a wandering fantasy, flight from the mother, the mother as salient, and machismo as psychological variables. CRs were not characterized by any of these variables. As with both groups of moderates, the father of the CRs was psychologically salient, but unlike the moderates, CTs perceived their fathers as flawed. The possible dynamic meaning of these configurations is discussed, as are their possible relationship to radical behavior and radical political ideology.This study was supported by grants from the American Jewish Committee and The National Science Foundation (GS35307A).Director of Resident Education, McLean Hospital. M.D., Harvard Medical School; residency training at Boston Veteran's Administration Hospital and Beth Israel Hospital in psychiatry. Psychoanalytic training, Boston Psychoanalytic Institute. Major interest: depression.Director of Training for Psychology Interns. Ph.D., Brandeis, 1960; Master's degree, University of Illinois. Major interest: schizophrenia.Professor of Political Science, Smith College. Major interest: modern European history — applying psychoanalytic methods to historical and social problems like student activism. 相似文献
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Selwyn Stanley 《Journal of family violence》2008,23(8):767-776
This study compared 75 wives of alcoholics with an equal number of wives of non-alcoholics matched on key socio-demographic
variables, using a cross sectional ex-post facto research design. Instruments to assess the extent of conflict and danger
perceived by them in their marital relationship, besides the communication apprehension experienced towards their partners
were administered. Analyses showed that the wives of alcoholics as a group had higher levels of conflict, perceived more danger
and experienced more apprehension in relating with their spouses, than the subjects of the reference group. Results indicate
the need to consciously focus on the spouse of the alcoholic and to help her resolve various marital issues with which she
is faced. Implications for therapeutic intervention with couples in alcohol complicated relationships to enable them overcome
their marital life deficits are discussed.
This study was funded by the University Grants Commission of India.
An erratum to this article can be found at 相似文献
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There is a growing interest within universities in the operation of state and local governments, and in the services they provide. An example of this interest is the Program for Urban and Policy Sciences (UPS) at the State University of New York at Stony Brook.Since its inception two years ago, it has been the operating policy of the Program to direct its principal research activities to real and immediate problems facing state and local governments in the New York area. In each of the collaborative efforts undertaken with an outside agency, we have seen our role as that of both problem analysts and catalytic agents for bringing about change. This means our faculty and students actively participate in the policy formulation and policy implementation processes. A case study of a cooperative effort undertaken with the Environmental Protection Administration of New York City illustrates these ideas.We believe that a successful university program should combine research with real world experience; the university should encourage the faculty to broaden the scope of their activities; and students, particularly those from minorities, should be trained for professional careers as planning and program analysts.Work reported in this paper has been supported by the National Science Foundation under grant GI-5. A similar version of the paper was presented at the American Political Science Association Conference, September 6–11, 1971, Chicago, Illinois and at the ORSA Conference, October 28, 1971, Anaheim, California. 相似文献