The utility of whole genome amplification for typing compromised forensic samples

Biological evidence has become invaluable in the crime laboratory; however, it may exist in limited quantity and/or quality. Given this, the ability to amplify total DNA obtained from evidence, in an unbiased manner, would be highly advantageous. Methods for whole genome amplification (WGA) have the potential to fulfill this role, resulting in a virtually unlimited supply of DNA. In the research presented, two WGA methods, improved primer extension preamplification and multiple displacement amplification (MDA), were tested using commercial kits. Control DNA, artificially degraded DNA, and DNA from fresh blood, aged blood, hair shafts, and aged bones underwent WGA, followed by short tandem repeat and mitochondrial DNA analysis. The methods did amplify DNA, but performed poorly on forensically relevant samples; the maximum amplicon size was reduced, and MDA often resulted in extraneous bands following polymerase chain reaction. Taken together, WGA appears to be of limited forensic utility unless the samples are of a very high quality.     

Toward a more comprehensive understanding of violence against impoverished women

Research and knowledge of violence against impoverished women continues to be limited. To achieve a more comprehensive understanding of violence against impoverished women and therefore inform prevention and intervention efforts for this population, the authors report on recent (past 6 months) physical, sexual, and psychological violence among 898 women who were randomly sampled from temporary shelter settings (n = 460) and low-income housing (n = 438) in Los Angeles County. Women experienced notable rates of violence during the past 6 months (e.g., 23% of sheltered women and 9% of housed women reported physical violence). Perpetrators were diverse, particularly for the sheltered women, including sexual partners, family, and strangers. These findings, and others suggesting that the different types of violence are distinct and severe, may call for more comprehensive screening and intervention efforts to enhance the safety of impoverished women.     

Parent–Child Relations and Adolescent Self-Image Following Divorce: A 10 Year Study

This Australian study explored the links between self-image, family structure (divorced or intact), parent–child relations, and gender at 3 intervals over 10 years during adolescence (mean ages 14.7 at Time 1, 17.9 at Time 2) to early adulthood (mean age 24.9 at Time 3). The sample comprised 37 families at the point of divorce when interviewed in 1981–82, and 41 intact families of similar age, gender, and socioeconomic background. Initial measures of self-image (Offer Self-Image Questionnaire: OSIQ) and parent–child relations (Parent Bonding Inventory: PBI) were repeated in 1985 and 1990–91. ANOVAs showed that divorced fathers were perceived as significantly less caring than those from intact families at each interval. There were no family group differences on the control scale, nor on either scale for mothers. Correlations between PBI scales and OSIQ were significant for the sample as a whole, but were stronger for those from intact than divorced families. ANOVAs showed that when mothers' and fathers' parenting styles were seen as highly caring and also not over controlling (i.e., optimal), adolescent self-image was significantly better, irrespective of gender or family structure. Examination of the joint influence of both parents showed that at Times 1 and 2 adolescent self-image was significantly better when one or both parents, compared with neither, were optimal. At Time 3, this comparison was significant only when both parents were optimal. Self-image was related to whether or not a custodial parent was seen as optimal but not to the gender of the child or custodial parent.     

Effects of Past Abuse Experiences on Children's Eyewitness Memory

Relations between child maltreatment and children's eyewitness memory were examined. A matched sample of abused and nonabused 3- to 10-year-old children (n = 70) participated in a play session with an unfamiliar adult and were interviewed about the interaction 2 weeks later. Consistent with results from previous research, older compared to younger children's reports were more complete and accurate. Abused and nonabused children performed similarly with several exceptions: Nonabused children were more accurate in answering specific questions, made fewer errors in identifying the unfamiliar adult in a photo identification task, and (at least for younger boys) freely recalled more information. Most effects remained when group differences in IQ and behavioral symptomology were statistically controlled. Importantly, abused and nonabused children did not differ in their accuracy or suggestibility in response to questions that were relevant to abusive actions. Among abused children, however, those who suffered more severe sexual abuse made more omission errors to specific abuse-relevant questions. Contributions to psychological theory and legal implications for understanding children's eyewitness memory and testimony are discussed.