A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis

As a first step in developing a molecular method for the individualization of marijuana samples, we evaluated a plant DNA extraction kit. The QIAGEN plant DNeasy method uses a spin column format for recovery of DNA and is effective for obtaining high molecular weight DNA from leaf, flower (bud), and seed samples of marijuana. The average DNA yield was 125-500 ng per 100 milligrams of fresh plant tissue. The recovered DNA was of polymerase chain reaction (PCR) quality as measured by the ability to generate reproducible amplified fragment length polymorphism (AFLP) profiles. AFLP is a technique used to create a DNA profile for plant varieties and is being applied to marijuana samples by the authors to link growers and distributors of clonal material. The QIAGEN plant DNeasy method was simple, efficient, and reproducible for processing small quantities of marijuana into DNA.     

Recent developments in medicine and law

This article focuses on certain noteworthy legislation and cases affecting medicine and law during the period September 1, 2001, to August 31, 2002. It does not attempt to discuss every case or piece of legislation, but instead seeks to summarize some of the more significant developments in this area of law.     

Animal cruelty motivations: assessing demographic and situational influences

Few studies have examined childhood and adolescent animal cruelty motives. Using a sample of 261 inmates surveyed at both medium and maximum security prisons ina southern state, the present study examined the impact of demographic attributes and situational factors relating specifically to a range of animal cruelty motivations. Almost half of the inmates who engaged in animal abuse reported committing some of the acts out of anger, whereas more than a third did so for fun. Regression analyses revealed that the most statistically salient variable in 7 of the 10 motivational models was whether animal cruelty was committed alone. Respondents who reported hurting or killing animals alone were more likely to commit the acts out of anger but less likely to have committed them to impress others, for sex, or to imitate others.     

Frame the Consolidation Debate with a Sound Argument: A Reply to a Response

The most persuasive argument in favor of city–county consolidation has been—and remains—economic development through the formation of a strong and attractive regional community identity. Empirical evidence consistently demonstrates that appeals to voters based on reducing inner-city/suburban socioeconomic inequity and enhancing administrative efficiency are a recipe for failed consolidation campaigns.     

Reciprocity in Adolescent and Caregiver Violence

Over a 2-year period, with assessments every six months, the reciprocity in violent behaviors (verbal and physical) was investigated in a sample of 161 adolescents, who met the criteria for substance or alcohol abuse or dependence, and their caregivers, who participated in a clinical trial for family treatment for adolescent substance abuse. Using observed variables in a structural equation model with panel data, there was very little stability in violent behaviors across time from the perspectives of both the adolescents and caregivers. Evidence for violence reciprocity between adolescent and caregiver was demonstrated toward the end of the study period. The results are discussed in the context of previous literature about adolescent-to-parent violence.     

Teaching Experientially in the Undergraduate Community Psychology Classroom

Experiential learning is a useful teaching tool in the undergraduate community psychology classroom. In addition to improving student outcomes, experiential learning is particularly relevant for community psychology, as it aligns with several core values of the field and can prompt not only student learning, but also civic engagement, social justice, and community betterment. In this article, we provide an overview of the themed issue on “Experiential Teaching Practices in Undergraduate Community Psychology.” The issue contains a variety of experiential teaching examples that fall into three clusters: (a) individual and group service-learning exercises; (b) using community experiences to augment in-class learning outside of a service-learning context; and (c) ways of having students draw on prior out-of-class or in-class community experiences to increase student understanding.     

Contrast Enhancement of Bite Mark Images Using the Grayscale Mixer in ACR in Photoshop®

Enhanced images may improve bite mark edge definition, assisting forensic analysis. Current contrast enhancement involves color extraction, viewing layered images by channel. A novel technique, producing a single enhanced image using the grayscale mix panel within Adobe Camera Raw^®, has been developed and assessed here, allowing adjustments of multiple color channels simultaneously. Stage 1 measured RGB values in 72 versions of a color chart image; eight sliders in Photoshop^® were adjusted at 25% intervals, all corresponding colors affected. Stage 2 used a bite mark image, and found only red, orange, and yellow sliders had discernable effects. Stage 3 assessed modality preference between color, grayscale, and enhanced images; on average, the 22 survey participants chose the enhanced image as better defined for nine out of 10 bite marks. The study has shown potential benefits for this new technique. However, further research is needed before use in the analysis of bite marks.