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991.
992.
The alleles present at the HLA DQ alpha locus may be typed by either allele specific oligonucleotide (ASO) probing or by restriction mapping, referred to here as amplification restriction fragment polymorphism (ARFP) analysis. ASO typing relies upon hybridization principles, whereas ARFP typing relies upon restriction site analysis. Dot-blot ASO typings which are of doubtful interpretation may be directly checked by ARFP analysis. Aliquots of the PCR products amplified using the commercial Amplitype HLA DQ alpha system are digested with suitable restriction endonucleases. Electrophoresis, blotting and detection of biotin-labelled restriction fragments provides a sensitive and robust typing method suited to forensic analysis.  相似文献   
993.
Horn  Jörg 《Asia Europe Journal》2004,2(4):573-587
Federated micro-regions are constituent units of federations having a maximum population of 300,000. Many are ethnically based. Although not larger than cities, these 55 territories are, constitutionally speaking, equal entities of their federations and have in some instances even shaped their own foreign policy. This article provides an analysis of their ability to withstand structural constraints and take advantage of the opportunities which smallness offers. In the first part, it discusses problems related to economies of scale and identifies a trend to establish new rather than merge existing federated micro-regions. The second part highlights external relations activities of some federated micro-regions and explains their partly pragmatic, partly political motivation.  相似文献   
994.
995.
Journal editors serve a vital, powerful role in academic fields. They set research priorities, serve as gatekeepers for research, play a critical role in advancing junior scholars as reviewers and eventually into editorial roles, build extensive networks, and gain valuable insight into the behavior and preferences of reviewers and scholars. This article analyzes data collected from leading public administration journals in 2017 to investigate the role of women as gatekeepers of public administration knowledge. The data illustrate a clear underrepresentation of women on editorial boards. Drawing from these data, research on journal editorships, and feminist theory, the authors present a critique of the current state of public administration research and a discussion of a way forward. They conclude with a proposal for how all public administration scholars (junior, senior, men, and women), journal leadership, and academic departments can move toward increasing women's representation in these important positions.  相似文献   
996.
Public Choice - It is well established in the literature that the number of interest group organizations varies across countries and states, with economic freedom and other institutional factors...  相似文献   
997.
Central governments face compliance problems when they rely on local governments to implement policy. In authoritarian political systems, these challenges are pronounced because local governments do not face citizens at the polls. In a national‐scale, randomized field experiment in China, we test whether a public, non‐governmental rating of municipal governments' compliance with central mandates to disclose information about the management of pollution increased compliance. We find significant and positive treatment effects on compliance after only one year that persist with reinforcement into a second post‐treatment year. The public rating appears to decrease the costs of monitoring compliance for the central government without increasing public and media attention to pollution, highlighting when this mode of governance is likely to emerge. These results reveal important roles that nonstate actors can play in enhancing the accountability of local governments in authoritarian political systems.  相似文献   
998.
This research provides new theoretical and empirical insights into the gender politics of the springboards to chief executive office. The extremely masculinised composition of the relatively few top national executive positions has posed a serious impediment to empirically assessing the conditions that may facilitate women's under-representation and men's over-representation. To overcome this constraint, this study looks at the top regional executive office across four West European countries that present a multilevel state structure – namely Austria, Germany, Spain and the United Kingdom. Using two original datasets, the article examines the ways in which the selection and reselection of regional prime ministerial candidates is shaped by individual, organisational and institutional factors that produce heterogeneous experiences and career opportunities across sex. The results show that women have not shattered the glass ceiling at the regional level and pinpoint the fact that they are held to higher standards, benefit less from the political resources they possess and are more dependent on the decision environment in which parties select executive candidates. The conclusion is that the rules of the game guiding selection and reselection processes are strongly biased towards men.  相似文献   
999.
Public administrators have long treated their connections with citizens as tools for efficiently achieving results. As Dwight Waldo once commented, their key political concern has been, “How much democracy can we afford?” Increasing use of social media technologies appears to expand citizen input at greatly reduced cost. On the basis of phenomenological investigation of virtual citizen–government relationships, we argue that the political consequences of social media have yet to be noticed fully. Social media exile citizens to Main Street—everyday life—instead of fostering connections in public space.  相似文献   
1000.
This paper explores the connections between education for youth civic engagement and theories and strategies from public health (specifically, epidemiology). We illustrate this with four applications of epidemiologic theory to youth civic engagement: social determinants and fundamental causes, vulnerable populations and cumulative disadvantage, positive spillover, and herd immunity and critical mass. Formalizing concepts of current civics, in schools and the public, as a civic epidemic, we present a case for individual‐ and group‐level interventions based around targeted, school‐based, effective civic education initiatives. Grounded in epidemiological theory, such approaches call attention to the simultaneous need to improve broad civics education and ensure that particular populations receive necessary attentions.  相似文献   
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