Analysis of 4‐Bromo‐2,5‐DimethoxyphenethylamineAbuser's Urine: Identification and Quantitation of Urinary Metabolites

The metabolites of 4‐bromo‐2,5‐dimethoxyphenethylamine (2C‐B), a psychoactive drug with hallucinogenic activity, were investigated in a urine sample from a user of 2C‐B. The urine sample was deconjugated enzymatically and the metabolites were recovered by liquid–liquid extraction. The extract was analyzed by gas chromatography/mass spectrometry after derivatization, and the results were used to identify and quantitate the metabolites. 4‐Bromo‐2,5‐dimethoxyphenylacetic acid was the most abundant metabolite of 2C‐B in human urine and accounted for 73% of the total amount of detected metabolites, followed by 4‐bromo‐2‐hydroxy‐5‐methoxyphenylacetic acid (13%) and 4‐bromo‐2,5‐dimethoxyphenylethyl alcohol (4.5%). According to the literature, the main metabolites of 2C‐B in rat urine are N‐(4‐bromo‐2‐methoxy‐5‐hydroxyphenylethyl)acetamide and N‐(4‐bromo‐2‐hydroxy‐5‐methoxyphenylethyl)acetamide. However, these metabolites accounted for only a small proportion of the total amount of detected metabolites in human urine, which indicates that there are significant species‐specific differences in the metabolism of 2C‐B. 4‐Bromo‐2,5‐dimethoxyphenylacetic acid, which was the most abundant metabolite in human urine, is thought to be generated by deamination of 2C‐B by monoamine oxidase (MAO) followed by oxidation by aldehyde dehydrogenase. Our results suggest that MAO plays a crucial role in the metabolism of 2C‐B in humans.     

A method for screening for various sedative-hypnotics in serum by liquid chromatography/single quadrupole mass spectrometry

A screening method for the detection of sedative-hypnotics in serum is described. The target drugs, which include practically all the sedative-hypnotics distributed in Japan, consisted of 5 barbiturates, 30 benzodiazepine-related drugs and 11 other sedative-hypnotics (i.e., apronalide, bromisovalum, chloral hydrate, triclofos, chlorpromazine, promethazine, diphenhydramine, hydroxyzine, zopiclone, zolpidem and tandospirone). Thirty-nine analytes, selected in terms of the pharmacokinetics of the target drugs, in human serum were screened using a combination of mixed-mode solid-phase extraction and liquid chromatography/electrospray-ionization single-quadrupole mass spectrometry. The detection limits (non-basic analytes, 1-50 ng/ml; basic analytes, 0.1-5 ng/ml) were sufficient to permit the screening of a single therapeutic administration of a target drug.     

Politics in Indonesia: Current State and Future Outlook

Indonesia's sixth and first directly elected president, Susilo Bambang Yudhoyono, and his vice president, Jusuf Kalla, symbolically represent the social power of the military and business elite who was nurtured during the Suharto era. The “Unity Cabinet” of the new administration reflects not just the division of labor between the president and vice-president, but the division of power among contending parties that make up the still-mutating ruling and opposition alliances. The subsequent neutralizing of the leading Muslim social organization Nahdlatul Ulama, the collapse of the opposition alliance, and personnel changes in the military have given the current administration a measure of stability, even as the challenges of implementing a lasting solution to the conflict in Aceh and of putting Indonesia back on the track of viable economic growth depend on the as-yet untested leadership of the president.     

Preliminary study of post mortem identification using lip prints

Identification using lip prints was first performed in the 1950s and was the subject of much research in the 1960s and 70s, leading to the acceptance of this technique as evidence in the criminal justice system. Previous research has focused on identifying lip print types or on methods of obtaining hidden lip prints left at the crime scene. The present study aimed to clarify characteristics of lip prints from cadavers with various causes of death (including drowning and hanging) and to determine the effects of fixation on post mortem changes in lip impressions.     

The study of international relations in Japan: towards a more international discipline

We deploy Waever’s sociology of science approach in a criticalcomparison of the American and Japanese international relations academies.We argue that there are four great debates in the American academy, andfour traditions of scholarship in the Japanese academy. We describethese debates and traditions in some detail, and identify andexplain points of contact and difference between the two respectiveacademies. We conclude by making a general case for methodologicalpluralism and offer reasons why the Japanese international relationsacademy is keen to sponsor a journal which is, in the wordsof Waever, able to ‘draw on national traditions while keepingup with American developments’.     

New Japan–China Relations and the Corresponding Positioning of the United States—History,Values, Realism in a Changing World

After more than a decade of deterioration in the relations between Japan and China symbolized by the visit of then-Prime Minister Junichiro Koizumi to Yasukuni Shrine, the relationship finally turned around in 2006–2007 into a qualitatively different one preparing for a new regional order. This was made possible mainly by the realistic perceptional change of the Japanese public on the regional security environment and the corresponding policy change of the Chinese Government on Japan-related histoty. The USA, as an essential stakeholder in Japan–China relations, also has to change itself in order to maintain its presence and interests in Asia and grasp a golden opportunity to build a more stable regional and international order given the drastic change in Japan–China relations which are still fragile due to structural problems. To this end, the USA needs to reevaluate and understand well the changing Japanese perception of history and of the severe security situation around Japan. Also, a Japan–USA–China trilateral partnership framework is now necessary to create a more stable Asian order by a USA initiative.     

Differentiated Citizenship and Ethnocultural Groups: A Japanese Case

It is a widely shared view that Japan is a culturally homogeneous country. This view is often deployed as justification for certain policy orientations to preserve cultural homogeneity. The goal of this article is to show that this line of thought is not acceptable on empirical and normative grounds. By considering two representative ethnocultural minorities—Ainu and Koreans—in terms of cultural, social, and political rights, I illustrate that there exists not only a plurality of ethnocultural groups in Japan with distinct claims to differentiated citizenship but also institutions and practices accommodating them. Moreover, a set of principles of differentiated citizenship underlying those institutions and practices are outlined. Finally, on the basis of the foregoing analysis, I argue that public policies attempt to preserve a cultural homogeneity that does not exist and such political aims as “enhancing social unity” are morally and prudentially undesirable.     

Measurement of Postmortem 1,5‐anhydroglucitol in Vitreous Humor for Forensic Diagnosis

In forensic diagnosis, postmortem blood glucose is known to be susceptible to change after death. However, the 1,5‐anhydroglucitol (1,5‐AG) concentrations in plasma and cerebrospinal fluid (CSF) reflect the mean blood glucose level for a short period of time. In this study, we compared the postmortem 1,5‐AG concentrations in vitreous humor and CSF in 47 subjects to evaluate the utility of this concentration in the vitreous humor for forensic diagnosis. The postmortem 1,5‐AG concentrations in vitreous humor (mean±SD: 20.2 ± 8.7 μg/mL) and CSF (16.8 ± 8.7 μg/mL) did not differ significantly and showed a strong correlation (r² = 0.87, p < 0.01). These results suggest that the vitreous humor 1,5‐AG concentration provides useful information on the antemortem blood glucose level, in addition to the HbA1c value and the CSF 1,5‐AG concentration.     

Synthesis and Analysis of Glucuronic Acid‐Conjugated Metabolites of 4‐Bromo‐2,5‐Dimethoxyphenethylamine

In the study reported here, two glucuronic acid‐conjugated metabolites of 4‐bromo‐2,5‐dimethoxyphenethylamine (2C‐B)—a ring‐substituted psychoactive phenethylamine—were chemically synthesized for the first time and a method for analyzing them in urine was developed. β‐D‐Glucuronide of 4‐bromo‐2,5‐dimethoxyphenylethylalcohol was successfully synthesized using methyl 2,3,4‐tri‐Ο‐acetyl‐1‐O‐(trichloroacetimidoyl)‐α‐D‐glucuronate as a glucuronyl donor and boron trifluoride diethylether complex as a Lewis acid catalyst. β‐D‐Glucuronide of 4‐bromo‐2,5‐dimethoxyphenylacetic acid was synthesized by condensing 4‐bromo‐2,5‐dimethoxyphenylacetic acid and benzyl D‐glucuronate followed by benzyl group deprotection based on catalytic hydrogenation. Two glucuronic acid‐conjugated metabolites of 2C‐B in urine were qualitatively and semiquantitatively evaluated via direct liquid chromatography/mass spectrometry (LC/MS) analysis of a diluted urine sample. The simple method proposed is expected to be useful for studying the metabolic fate of 2C‐B.