发展文化生产力的当代路径--学习《中共中央关于加强党的执政能力建设的决定》

深化文化体制改革,解放和发展文化生产力,是加强党的执政能力建设的一项重要任务.当前和今后一个时期,应当把革除体制性障碍、加强队伍建设作为解放和发展文化生产力的基本路径.     

论法官与社区的关系——以“中国法官十杰”为考察对象

中国法官十杰的选评，昭示了法官与社区的关系，是亲密接触。学术界从司法权的特殊性出发，强调法官与社区应保持距离。探究法官与社区亲密接触和保持距离的缘由，正确平衡二者的关系，消解其矛盾，才能构建正确健康的法官与社区关系。     

中东国家核竞赛加剧及其影响

长期以来,阿拉伯国家一直积极致力于建设中东无核区。但近来随着中东地缘政治和安全形势的嬗变,尤其是伊朗核事态的发展,它们的态度急剧变化,越来越多的中东国家提出了核开发要求。这不仅使中东核不扩散机制面临严峻挑战,也将对该地区及全球政治和安全产生一系列重要影响。     

邓小平多党合作思想浅析

邓小平多党合作思想是对毛泽东统一战线思想的继承和发展,具有发展了新时期党对民主党派的基本方针、确立了民主党派的参政党地位、概括了新时期发扬民主的本质特征和基本形式,以及强调了加强制度建设对多党合作的重要性的特点。并指出,在新形势下,加强自身建设是执政党和参政党共同面临的任务。     

陈旧骨骼DNA提取

目的寻找从陈旧骨骼中提取DNA的有效方法。方法运用传统的有机法结合Microcon100纯化柱提取骨骼DNA。结果用常规荧光标记复合STR基因分型法可对提取到的陈旧骨骼DNA进行成功分析。结论有机法结合Micrcon100纯化柱提取陈旧骨骼DNA法可有效应用于实际检案。     

一代思想家毛泽东——论"毛泽东的思想"的真谛

毛泽东是大革命家、大政治家,也是大思想家.思想家有一套博大的思想体系,有一套独特的思维方式和行为方式.往往在其去世几十年之后,进行正本清源,才能理解其思想真谛.毛泽东以平等主义为核心思想,终生致力于反不平等、建立一种平等社会的斗争.平等涵盖公平正义,是培育和谐的土壤.平等主义是"毛泽东的思想"的真谛,是建设和谐社会与和谐世界的重要思想基础.     

炫耀背后:"非炫耀性消费"研究

自凡勃伦发表《有闲阶级论》以来,炫耀性消费的冲动几乎被视为"有闲阶级"天生的喜好.但笔者在一项实证调查中却发现,部分"有钱人"在消费过程中已经形成了与"歧视性对比"和"金钱竞赛"的炫耀特质相对立的、无意或有意不服从炫耀性消费的现象,本文谓之曰"非炫耀性消费",并从社会学的角度探求其消费决策动机.     

Application of HLA-DRB1 genotyping by oligonucleotide micro-array technology in forensic medicine

The human leukocyte antigen (HLA) system is known to be the most complex polymorphic system in the human genome. Among all of the HLA loci, HLA-DRB1 has the second largest number of alleles. The purpose of this study is to develop an oligonucleotide micro-array based HLA-DRB1 typing system for use in forensic identification, anthropology, tissue transplantation, and other genetic research fields. The system was developed by analyzing the HLA-DRB1 (DRB1) genotypes in 1198 unrelated healthy Chinese Han individuals originating from various parts of China and residing in Shanghai, China. METHOD: Polymerase chain reaction (PCR) coupled with the oligonucleotide micro-array technology was used to detect and type HLA-DRB1 alleles of the sample individuals. The reliability, sensitivity, consistency and specificity were evaluated for use in forensic identification. Furthermore, a meta-analysis was carried out by comparing the allele frequencies of the HLA-DRB1 locus with those of other Chinese Han groups, Chinese minorities and other ethnic populations. RESULTS: All the DNA samples yielded a 273 bp amplification product, with no other amplification products in this length range. The minimum quantity of DNA detected by this method is 15 ng in a PCR reaction system of 25 microl. The population studied appeared to be not in Hardy-Weinberg equilibrium. Observed heterozygosity (Ho), expected heterozygosity (He), expected probability of exclusion (PE), polymorphic information content (PIC), and discrimination power (DP) of the HLA-DRB1 locus from the Shanghai Han ethnic group were evaluated to be 0.8022, 0.8870, 0.7741, 0.8771, 0.9750, respectively. A total of 25 HLA-DRB1 alleles were identified. HLA-DRB1*09XX, *04XX, *12XX and *15XX were the most frequent DRB1 alleles, which were observed in 58.76% of the sample. One hundred and sixteen genotypes were found. The five most frequent genotypes were: *04XX/*04XX (0.0626), *09XX/*09XX (0.0593), *04XX/*09XX (0.0551), *09XX/*15XX (0.0384) and *08XX/*12XX (0.0351). The meta-analysis showed that there were uniquely distributed features of DRB1 alleles among various ethnic populations and among the studied population groups from various regions with the same ethnic origin. CONCLUSIONS: An HLA-DRB1 genotyping system has been developed and established based on the oligonucleotide micro-array technology. The HLA-DRB1 typing of the Han population in Shanghai has revealed a relatively high heterogeneity. Information obtained in this study will be useful for medical and forensic applications as well as in anthropology research. Large-scale micro-array detection is highly accurate and reliable for DNA-based HLA-DRB1 genotyping. These results suggest that HLA-DRB1 DNA polymorphisms and the database of the Shanghai Han group have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.     

Four X-chromosomal STRs and their allele frequencies in a Chinese population

X-chromosomal markers are particularly useful for solving complex problems relating to kinship and deficiency paternity analysis. In the present study, population genetics data of four X-chromosomal short tandem repeats (STRs) DXS8378, HPRTB, DXS7423 and DXS7132 were obtained by analyzing 500 unrelated Chinese individuals (250 males and 250 females). Population genetics data obtained from the Chinese were compared with those of the Asian and European population. Mutations in X-chromosomal markers were analyzed based on a total of 61 proven father-child and 45 proven mother-child pairs.     

“十一五”贵州主导产业的选择和培育

本文运用区域主导产业选择"四基准",对贵州传统主导产业进行评价,指出"十一五"期间要继续壮大部分传统主导产业,使其对贵州经济产生更大的带动作用;同时,提出要培育部分新兴主导产业。